한국환경보건학회지 (Journal of Environmental Health Sciences)

  • 제34권1호
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  • Pages.55-61
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  • 2008
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  • 1738-4087(pISSN)
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  • 2233-8616(eISSN)
한국환경보건학회 (Korean Society of Environmental Health)
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목향 추출물의 항산화 및 항암활성

Antioxidative and Antitumor Activity of Extracts from Saussurea lappa

  • 송진욱 (계명대학교 자연과학대학 공중보건학과) ;
  • 민경진 (계명대학교 자연과학대학 공중보건학과) ;
  • 차춘근 (전통미생물 자원개발 및 산업화연구센터)
  • Song, Jin-Wook (Department of Public Health Graduate School Keimyung University) ;
  • Min, Kyung-Jin (Department of Public Health Graduate School Keimyung University) ;
  • Cha, Chun-Geun (The Center for Traditional Microorganism Resources, Keimyung University)
  • 발행 : 2008.02.28
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초록

This study was carried out to investigate the antioxidative and antitumor activities of Saussurea lappa for the purpose of developing a functional food. The methanol extract of Saussurea lappa was fractionated with five solvents (hexane, chloroform, EtOAc, BuOH, water) and examined for antioxidative activities and xanthine oxidase inhibitory activity in addition to growth inhibitory activity of human cancer cell (HT-29, SNU-1, HeLa). Total phenol compound contents were higher in EtOAC fraction than other fractions. Also, electron donating ability was over 90% at $500{\mu}g/ml$ (93.1 %) and $1000{\mu}g/ml$ (94.3%). The hexane fraction revealed stronger nitrite scavenging ability than the positive control (ascorbic acid) and its abilities were 22.4% and 42.8% at $500{\mu}g/ml$ and $1000{\mu}g/ml$, respectively. Xanthine oxidase inhibitory activity had similar tendency to electron donating ability. The EtOAc fraction showed high inhibition to xanthien oxidase activities at $500{\mu}g/ml$(81.9%) and $1000{\mu}g/ml$(90.4%). In the antitumor activity test, the hexane fraction exhibited potent growth inhibition activity against HT-29, SNU-1 and HeLa cells. Therefore, we believe that Saussurea lappa can be developed into a functional food with antioxidant activity. Additional studies are required for the hexane and chloroform fractions of Saussurea lappa to develop them into therapeutic supplements for stomach cancer, colon cancer, and cervical cancer.

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참고문헌

  1. Yu, H. E., Leaniza, M. M., Bae, Y. J., Lee, D. H., Park, J. S ., Kwak, H. S ., Kim, H. K. and Lee, J . S. : Screening and extraction condition of antiaging bioactive substances from medicianl plants. Journal of the Korean Society of Food Science and Nutrition, 34(8), 1136-1142, 2005 https://doi.org/10.3746/jkfn.2005.34.8.1136
  2. Branen, A. L. : Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytolune. Journal of the American Oil Chemists' Socitey, 52, 59- 65, 1975 https://doi.org/10.1007/BF02901825
  3. Farag, R. S., Badei, A. Z. M. A. and Baroty, G. S. A. : Influence of thyme and clove essential oils in cotton seed oil oxidation. Journal of the American Oil Chemists' Socitey, 66, 800-806, 1989 https://doi.org/10.1007/BF02653671
  4. Ziegler, D. W., Hutchison, H. D. and Kissing, R. F. : Induction of xanthine oxidase by virus infections in newborn mice. Infection and Immunity, 3, 237-242, 1971
  5. Duke, E. J., Joyce, P. and Ryan, J. P. : Characterization of alternative molecular forms of xanthine oxidase in the mouse. Journal of Biochemistry, 131, 187- 194, 1973 https://doi.org/10.1042/bj1310187
  6. Ody, P. : Herbal insights : Aclose look at active constituents of medicinal hers. Soaps, Oils, Fats and Waxes Journal, 121, 8-11, 1995
  7. Lee, K. G., Mitchell, A. E. and Shibamoto, T. : Determination of antioxidant properties of aroma extracts from various beans. Journal of Agriculture and Food Chemistry, 43, 4817-4820, 2000
  8. Park, H. S., Min, K. J., Cha, C. G., Song, J. W. and Son, J. C. : Antimicrobial activities against oral microbes and growth-inhibitory effect on oral tumor cell by extract of Paeonia lactiflora. Korean Journal of Environmental Health, 33(1), 21-29, 2007 https://doi.org/10.5668/JEHS.2007.33.1.021
  9. Jo, M. J. and Min, K. J. : Anti-microbial activites against oral microbes and growth-inhibitory effect on oral tumor cell of extracts of Perilla and Mugwort. Korean Journal of Environmental Health, 33(2), 115- 122, 2007 https://doi.org/10.5668/JEHS.2007.33.2.115
  10. Shin, M. K : Hebology, 1997
  11. Wedge, D. E., Galindo, J. C. and Macias, F. A. : Fungidal activity of natural and synthetic sesquiterpene lactone analogs. Phytochemistry, 53, 747-757, 2000 https://doi.org/10.1016/S0031-9422(00)00008-X
  12. Park, H. J., Basnet, W. T. P., Kadota, S. and Namba, T. : Syringin 4-O-beta-glucoside, a new phenylpropanoid glycoside, and costunolide, an nitric oxide synthase inhibitor, from the stem bark of Magnolia sieboldii. Journal of Natural Products, 59, 1128-1130, 1996 https://doi.org/10.1021/np960452i
  13. Matsuda, H., Toguchida, I., Ninomiya, K., Kageura, T., Morikawa, T. and Yoshikawa, M. : Effects of sesquiterpene and amino acid sesquiterpene conjugates from the roots of saussurea lappa on inducible nitri oxide synthase and heat shock protein in lipopolysaccharide activated macrophages. Bioorganic and Medicinal Chemistry, 11, 709-715, 2003 https://doi.org/10.1016/S0968-0896(02)00471-6
  14. Folin, O. and Denis, W. : On phosphotungastic phosphomolybdic compounds as color reagents. The Journal of Biological Chemistry, 12, 239-249, 1912
  15. Blois, M. S. : Antioxidant determination by the use of a stable free radical. Nature, 181, 1199-1200, 1958 https://doi.org/10.1038/1811199a0
  16. Gray, J. I. and Dugan, Jr L. R. : Inhibition of N-nitrosamine formation on model food system. Journal of Food Science, 40, 981-984, 1975 https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  17. Stripe, F. and Corte, E. D. : The regulation of rat liver xanthine oxidase. The Journal of Biological Chemistry, 244, 3855-3863, 1969
  18. Carmichael, J., De Graff, W. G., Gazder, A. F., Minna, J. D. and Mitchell, J. B. : Evaluation of tetrazoliumbased semiautomated colorimetic assay. Assesment of chemosensitivity testing. Cancer Research, 47, 936- 942, 1987
  19. Kumar, R. and Singh, M. : Tannins-Their adverse role in ruminant nutrition. Journal of Agricultal and Food Chemistry, 32, 447-453, 1984 https://doi.org/10.1021/jf00123a006
  20. Hertog, M. G. L., Sweetnam, P. M., Fehily, A. M., Elwood, P. G. and Kromhout, D. : Antioxidant flavonols and ischaemic heart disease in a Welsh population of men. The caerphilly study. The American Journal of Clinical Nutrition, 65, 1489-1494, 1997 https://doi.org/10.1093/ajcn/65.5.1489
  21. Choi, S. I., Lee, , Y. M. and Heo, T. R. : Screening of hyaluronidase inhibitory and free radical scavenging activity in vitro of traditional herbal medicine extracts. Korean Journal of Biotechnology and Bioengineering, 18(4), 282-288, 2003
  22. Park, C. S. : Antioxidative and nitrite scavenging abilities of medicianl plant extracts. Korean Journal of Food Preservation, 12(6), 631-636, 2005
  23. Hatano, T., Yasuhara, T., Yoshihara, R. and Okuda T. : Inhibitory effects of galloylated flavonoids on xanthine oxidase. Planta Medica, 57, 83-89, 1991 https://doi.org/10.1055/s-2006-960028
  24. Yeo, S. G., Park, Y. B., Kim, I. S., Kim, S. B. and Park, Y. H. : Inhibition of xanthine oxidase by tea extracts from green tea, oolong tea and black tea. Journal of the Korean Society of Food Science and Nutrition, 24(1), 154-159, 1995
  25. Choi, W. S., Kim, D. K., Lee, Y. H., Kim, J. E. and Lee, S. E. : Antioxidative and cytotoxicity activities of compounds isolated from Korean Phus verniciflua S.. Jouranl of the Korean Society for Applied Biological Chemistry, 45(3), 168-172, 2002
  26. An, B. J., Lee, J. T., Kwak, J. H., Park, J. M., Lee, J. Y., Son, J. H., Bae, J. H. and Choi, C. : Biological Activity of polyphenol group fraction from Korean pear peel. Jouranl of the Korean Society for Applied Biological Chemistry, 47(1), 92-95, 2004