Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Gene Flow of Oriental Fruit Moth, Grapholita molesta, Populations Analyzed by RAPD Molecular Markers

RAPD 분자지표를 이용한 복숭아순나방(Grapholita molesta)의 집단 유전적 변동 분석

  • Son, Ye-Rim (Majour in Plant Medicine, School of Bioresource Sciences, College of Natural Sciences, Andong National University) ;
  • Kim, Yong-Gyun (Majour in Plant Medicine, School of Bioresource Sciences, College of Natural Sciences, Andong National University)
  • 손예림 (안동대학교 자연과학대학 생명자원과학부 식물의학) ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학부 식물의학)
  • Published : 2008.03.30
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Abstract

Oriental fruit moth, Grapholita molesta, is a serious pest on apples. To control this pest in an environmentally friendly method, mating disruption strategy using sex pheromone has been developed. Area-wide application of mating disruption has been needed to be effective, with little understanding on how much size of apple cultivating area should be treated in one time application of the mating disruption technique. On this matter, we needed to determine a minimal mating active zone of G. molesta that should be applied with mating disrupters to be effective. Molecular markers to discriminate a specific population should be developed to trace population migration for reproductive behaviors. Here we developed two effective molecular markers using random amplified polymorphic DNA (RAPD) technique. Different field populations of G. molesta, based on locations and seasons, were analyzed with these markers. In a specific location, G. molesta populations varied in genetic composition with different seasons. Different local populations showed differential variation according to their relative distances among apple orchards. In overall, genetic variation among different populations became lessen with progression of seasons.

복숭아순나방(Grapholita molesta)은 사과에 주요 해충이다. 이 해충을 환경친화형 방법으로 방제하기 위해 성페로몬을 이용한 교미교란제 처리 기술이 개발되고 있다. 광범위한 영역에 대한 교미교란제의 처리는 복숭아순나방 방제에 효과적이나 이러한 광범위영역에 대한 구체적 크기를 결정하는 방법은 알려지지 않았다. 이를 결정하기 위해서는 복숭아순나방의 교미행동 범위를 결정할 수 있는 방법이 개발되어야 하며, 이를 위해 복숭아순나방의 이동을 추적할 수 있는 분자지표의 개발이 필요하게 되었다. 본 연구는 RAPD (random amplified polymorphic DNA) 기술을 이용하여 효과적인 두 개의 분자지표를 개발하였다. 상이한 지역과 시기에 따라 구분되는 야외 복숭아순나방 집단들에 대해서 이들 분자지표를 이용하여 집단 유전 분석이 실시되었다 이들 분자지표들은 특정지역에서 복숭아순나방 집단들이 시기적으로 유전적 조성에 뚜렷한 차이를 보여 주었다. 또한 서로 다른 지역의 복숭아순나방 집단들은 거리에 따라 상대적으로 차이가 증가하였지만, 계절이 진행함에 따라 그 차이가 감소하였다.

Keywords

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