Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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A Novel Kinesin-like Protein, Surhe is Associated with Dorsalization in the Zebrafish Embryos

  • Kim, Eun-Joong (School of Pharmacy, Seoul National University) ;
  • Ro, Hyun-Ju (NIH-NICHD-LMG) ;
  • Huh, Tae-Lin (Department of Genetic Engineering, Kyungpook National University) ;
  • Lee, Chang-Joong (Department of Biology, College of Natural Sciences, Inha University) ;
  • Choi, Jin-Hee (Faculty of Environmental Engineering, College of Urban Science, University of Seoul) ;
  • Rhee, Myung-Chull (Department of Biology, College of Biosystem Science, Chungnam National University)
  • Published : 2008.12.31
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Abstract

We are reporting the expression patterns and possible biological functions of a novel Kinesin-like protein, Surhe, in the zebrafish. Homology studies of derived amino acid sequences suggest that Surhe has an amino-terminal kinesin motor domain that is similar to that of the emerging MKLP-1 subfamily [Kim and Endow, 2000] and two coiledcoil domains in a central region. Cellular localization studies in mammalian cells revealed that Surhe protein is located in cytoplasm, suggesting that Surhe may be involved in the intracellular transport. During the developmental process, surhe transcripts are highly expressed in early embryonic stages. Overexpression of the dominant negative form of Surhe significantly down-regulates the dorsalization markers, such as goosecoid, bozozok, and chordin. Taken together, we postulate that Surhe may be involved in dorsalization process as a motor molecule.

Keywords

References

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