The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Detection for the Resistance of Fusarium spp. Isolated from Rice Seeds to Prochloraz and Cross-resistance to Other Fungicides Inhibiting Sterol Biosynthesis

벼 종자에서 분리한 Fusarium속 균주들의 prochloraz에 대한 저항성 검정 및 교차 저항성 조사

  • Shin, Myeong-Uk (Laboratory of Plant Fungal Pathogen, College of Agriculture, Life Science and Environment, Chungbuk National University) ;
  • Kang, Hyo-Jung (Chungbuk Agricultural Research and Extension Services) ;
  • Lee, Yong-Hwan (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Heung-Tae (Laboratory of Plant Fungal Pathogen, College of Agriculture, Life Science and Environment, Chungbuk National University)
  • 신명욱 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학) ;
  • 강효중 (충북농업기술원 시험연구부 농업환경과) ;
  • 이용환 (농업과학기술원 농업생물부) ;
  • 김흥태 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학)
  • Published : 2008.09.30
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Abstract

To assess the resistance to prochloraz, $EC_{50}$ values of Fusarium isolates obtained from rice seed were investigated through the agar dilution method. $EC_{50}$ value of 36 isolates of Fusarium spp. to prochloraz ranged from 0.020 to $1.78{\mu}g\;mL^{-1}$ with an average of $0.25{\mu}g\;mL^{-1}$. According to the species of Fusarium, the average $EC_{50}$ value was fluctuated; $0.091{\mu}g\;mL^{-1}$ for F. moniliformis, $0.11{\mu}g\;mL^{-1}$ for F. proliferatum and $0.31{\mu}g\;mL^{-1}$ for F. fujikuroi. The resistant baseline was decided at $0.5{\mu}g\;mL^{-1}$ to determine if the isolate was resistant to prochloraz or not. Based on the resistant baseline, the ratio of resistant isolates was 14%. There was no correlation between the resistance to prochloraz and the pathogenicity of Fusarium spp. on rice seedlings. The resistant isolates of F. fujikuroi did not show the cross-resistance to other sterol biosynthesis inhibiting fungicides, triflumizole, hexaconazole, difenoconazole and tebuconazole.

Fusarium spp. 균주들에 대한 Prochloraz의 살균효과를 비교하기 위하여 한천희석법을 사용하여 각 균주에 대한 prochloraz의 $EC_{50}$값을 구하였다. 전체 균주에 대한 평균 $EC_{50}$값은 $0.25{\mu}g\;mL^{-1}$이었으며, 최저값과 최고값은 0.02과 $1.78{\mu}g\;mL^{-1}$이었다. Fusarium의 종별로 조사한 평균 $EC_{50}$값을 보면 F. fujikuroi의 경우가 $0.31{\mu}g\;mL^{-1}$, F. moniliformis와 F. proliferatum의 경우는 0.09과 $0.11{\mu}g\;mL^{-1}$이었다. 실험에 사용한 36개 Fusarium 속 균주의 Prochloraz에 대한 감수성과 저항성을 판단할 수 있는 기준 $EC_{50}$ 값은 $0.5{\mu}g\;mL^{-1}$가 타당하며, 이 기준에 의한 저항성 균주의 분리 비율은 13.8%이었다. Prochloraz에 대한 저항성과 Fusarium 균주의 병원성간에는 전혀 상관관계가 없었다. 또한 prochloraz에 대해서 저항성인 균주들은 triflumizole, hexaconazole, difenoconazole, tebuconazole과 교차 저항성을 보이지 않았다.

Keywords

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