Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Bacteriocidal Effects of Ultraviolet Irradiation for Reducing Bovine Mastitis Derived from Environmental Contamination

우분뇨 유래 젖소 유방염 저감을 위한 자외선 조사 살균의 효과 규명

  • Kim, Dong-Hyeok (College of Veterinary Medicine, Gyeongsang National University) ;
  • Lim, Jung-Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Jin-Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Jang, Hong-Hee (College of Veterinary Medicine, Gyeongsang National University) ;
  • Jang, Dong-Il (Department of Bio-Industrial and Machinery, Chungnam National University) ;
  • Lee, Seung-Joo (Department of Bio-Industrial and Machinery, Chungnam National University) ;
  • Lee, Hu-Jang (College of Veterinary Medicine, Gyeongsang National University) ;
  • Min, Won-Gi (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kwon, Sun-Hong (Department of Bio-Industrial Machinery, Pusan National University) ;
  • Kim, Sang-Hun (College of Veterinary Medicine, Chungnam National University) ;
  • Oh, Kwon-Young (Department of Agricultural Engineering, National Academy of Agriculturral Science) ;
  • Kim, Suk (College of Veterinary Medicine, Gyeongsang National University)
  • Published : 2008.12.31
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Abstract

Bovine mastitis is an important disease causing serious economic loss in dairy production and food poison in public health. The major causative agents of bovine mastitis include Escherichia coli (E. coli), Streptococcus agalactiae (S. agalactiae), Staphylococcus aureus (S. aureus). These bacteria were found in milk and environmental condition such as feces, water, soil and so on. Recently, many cases of mastitis are derived from environmental contamination of micro-organisms, which important factors for the spread of this disease in farm. Ultraviolet irradiation (UV) has been used as disinfection for waste and water in clinical and industrial facilities. Moreover the UV irradiation has been used as useful bactericidal agents to remove bacterial biofilms in environmental condition. In this study, we determined the bacterial replication in different percentage of water content (PWC) in sterilized saw dust and feces complexes from farm, and results showed that slightly decreased growth pattern of E. coli and S. agalactiae but increased growth pattern of S. aureus in various PWC (200, 400 and 600%) until 144 h incubation. In the bacteriocidal effect of UV irradiation to bacteria in saw dust and feces complex, the results showed that bacteriocidal effect was depended on the UV irradiation time, irradiation distance and PWC. Especially the antibacterial activity of UV irratiation is stronger in low PWC (50%), long time irradiation (50 sec), and short distance (5 cm) than other condition of this study. Furthermore UV irradiation with stirring showed increased the bactericidal effect compared without stirring. These results suggested that bovine mastitis causing agents may survive long time in environmental condition especially saw dust and feces complexes in farm and can cause a various disease including mastitis. Moreover, these data can be used as basis for application and development of UV disinfection to control of bovine mastitis from environmental contaminated bacteria in dairy farm.

E. coli, S. agalactiae, S. aureus는 젖소의 유방염을 유발하는 주요 원인균들이다. 이 균들은 분변 혹은 우유에 존재하며, 감염되지 않은 다른 개체로의 감염을 유발한다. 자외선은 소독제로서 이미 산업계 및 의료계에서 쓰레기 및 물의 살균에 사용되고 있으므로, 자외선을 이용하여 젖소의 유방염 확산을 방지하는 것의 실효성을 검증하였다. 분변의 함수율은 젖소 유방염 유발균의 증식에 영향을 미치지 않았고, 144시간 이상의 장시간 생존이 가능함을 확인하였다. 자외선의 조사 시, 조사하는 동안 우분뇨 및 톱밥을 교반하는 것이 교반하지 않는 것보다 균의 증식을 억압하는 것을 확인할 수 있었다. 한편, 자외선의 살균력은 함수율이 낮을수록, 조사시간이 길수록, 조사거리가 짧을수록 더 증가하는 것을 확인할 수 있었다. 본 실험을 통해 얻은 함수율, 조사시간, 조사거리, 교반 여부에 대한 결과는 환경 유래, 특히 우분뇨 유래 젖소 유방염의 감염을 예방하고자 할 때 자외선 살균기의 적용이 가능할 것이며, 현장에 적용할 살균기의 제작에 중요한 기초 자료로서 활용될 것이다.

Keywords

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