Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Ecophysiological Changes in a Cold Tolerant Transgenic Tobacco Plant Containing a Zinc Finger Protein (PIF1) Gene

  • Yun, Sung-Chul (Department of Biomedical Sciences, Sun Moon University) ;
  • Kwon, Hawk-Bin (Department of Biomedical Sciences, Sun Moon University)
  • Published : 2008.12.31
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Abstract

The ecophysiological changes occurring upon cold stress were studied using cold tolerant transgenic and wild-type tobacco plants. In a previous study, cold tolerance in tobacco was induced by the introduction of a gene encoding the zinc finger transcription factor, PIF1. Gas-exchange measurements including net photosynthesis and stomatal conductance were performed prior to, in the middle of, and after a cold-stress treatment of $1{\pm}2^{\circ}C$ for 96 h in each of the four seasons. In both transgenic and wild-type plants, gas-exchange parameters were severely decreased in the middle of the cold treatment, but had recovered after 2-3 h of adaptation in a greenhouse. Most t-test comparisons on gas-exchange measurements between the two plant types did not show statistical significance. Wild-type plants had slightly more water-soaked damage on the leaves than the transgenic plants. A light-response curve did not show any differences between the two plant types. However, the curve for assimilation-internal $CO_2$ in wild-type plants showed a much higher slope than that of the PIF1 transgenic plants. This means that the wild-type plant is more capable of regenerating Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and has greater electron transport capacity. In conclusion, cold-resistant transgenic tobacco plants demonstrated a better recovery of net photosynthesis and stomatal conductance after cold-stress treatment compared to wild-type plants, but the ecophysiological recoveries of the transgenic plants were not statistically significant.

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References

  1. Xiong, L, Schumaker, K. S., and Zhu, J. K. (2002) Cell signaling during cold, drought, and salt stress, Plant Cell 14, S165-S183 https://doi.org/10.1105/tpc.010278
  2. Seki, M., Narusaka, M., Abe, H., Kasuga, M., Yamaguchi, K., Carninchi, P., Hayashizaki, Y. and Shinozaki, K. (2001) Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray, Plant Cell 13, 61-72 https://doi.org/10.1105/tpc.13.1.61
  3. Zhu, T. and Provart, N. J. (2003) Transcriptional responoses to low temperature and their regulation in Arabidopsis, Can. J. Bot. 81, 1168-1174 https://doi.org/10.1139/b03-115
  4. Thomashow, M. F. (1999) Plant cold acclimation: Freezing tolerance genes and regulatory mechanism, Annu. Rev. Plant Physiol. Plant Mol. Biol. 50, 571-599 https://doi.org/10.1146/annurev.arplant.50.1.571
  5. Qiang, L, Nanming, Z, Yamaguchi-Shinozaki, K. and Shinozaki, K. (2000) Regulatory role of DREB transcription factors in plant drought, salt and cold tolerance, Chinese Sci. Bull. 45, 970-975 https://doi.org/10.1007/BF02884972
  6. Hwang, E. W., Kim, K. A., Park, S. C., Jeong, M. J., Byun, M. O. and Kwon, H. B. (2005) Expression profiles of hot pepper (Capsicum annuum) genes under cold stress condition, J. Biosci. 30, 657-667 https://doi.org/10.1007/BF02703566
  7. Hwang, E. W., Park, S. C., Byun, M. O., Choi, M., and Kwon, H. B. (2008) Overexpressing of zinc finger protein of Capsicum annuum (PIF1) in tobacco enhances cold tolerance, Genes & Genomics. 30, 93-99
  8. Hopkins, W. G. and Huner, N. P. A. (2004) Plant physiology, John Wiley & Sons, Inc., Hoboken, New Jersey, USA, p. 187-198
  9. Kim, J. C., Lee, S. H., Cheong, Y. H., Yoo, C. M., Lee, S. I., Chun, H. J., Yun, D. J., Hong, J. C., Lee, S. Y., Lim, C. O. and Cho, M. J. (2001) A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants. Plant Journal 25(3), 247-259 https://doi.org/10.1046/j.1365-313x.2001.00947.x
  10. Sakamoto, H., Maruyama K, Sakuma, Y., Meshi, T., Iwabuchi, M., Shinozaki, K. and Yamaguchi-Shinozaki, K. (2004) Arabidopsis Cys2/His2-type zinc-finger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions, Plant Physiol. 136, 2734-2746 https://doi.org/10.1104/pp.104.046599
  11. Mukhopadhyay, A., Vij, S. and Tyagi, A. K. (2004) Overexpression of a zinc-finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco, Proc. Acad. Sci. USA 101, 6309-6314
  12. Huang, J., Wang, J. F., Wang, Q. H. and Zhang, H. S. (2005) Identification of a rice zinc finger protein whose expression is transiently induced by drought, cold but not by salinity and abscisic acid, DNA Seq., 16, 130-136 https://doi.org/10.1080/10425170500061590