Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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cDNA Microarray Analysis of Gene Expression in Pig Spleen Lymphocytes in Response to Extract of Raspberry

분자 추출물을 돼지의 비장 면역세포에 처리시 cDNA Microarray를 이용한 유전자 발현분석

  • Chung, Chung-Soo (Department of Animal Science, Chungbuk National University) ;
  • Choi, Young-Sook (Department of Animal Science, Chungbuk National University) ;
  • Lim, Hee-Kyong (Graduate School of Biotechnology & Information Technology, Hankyong National University) ;
  • O, Yun-Genel (Graduate School of Biotechnology & Information Technology, Hankyong National University) ;
  • Mandal, Prabhat Kumar (School of Biotechnology, Hankyong National University) ;
  • Choi, Kang-Duk (School of Biotechnology, Hankyong National University)
  • 정정수 (충북대학교 축산학과) ;
  • 최영숙 (충북대학교 축산학과) ;
  • 임희경 (한경대학교 생물환경, 정보통신전문대학원) ;
  • 오윤길 (한경대학교 생물환경, 정보통신전문대학원) ;
  • ;
  • 최강덕 (한경대학교 생명공학부)
  • Published : 2008.12.31
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Abstract

The present study was undertaken to investigate specific immune response of Rubus coreanus Miquel (raspberry) in pig spleen lymphocytes and gene expression induced by the extracts of raspberry using gene chip technology. The 70% ethyl alcohol extracts of raspberry were treated to pig spleen lymphocytes. The extracts of raspberry stimulated the proliferation of splenocytes and increased the population of CD3 & CD4 T-cells and B-cells in pig spleen lymphocytes. The extracts of raspberry improved immune response by increasing the viability of splenocytes. In microarray study we found eight genes were significantly up- regulated by the extracts of raspberry in pig splenocytes, including genes known to be involved in cell structure and immune response, particularly microtubule-associated protein 4, cytoplasmic dynein heavy chain, tumor necrosis factor alpha, lymphotoxin-beta receptor precursor. However, ten genes were down- regulated by the extracts of raspberry treatment.

본 연구에서는 복분자(라즈베리)의 돼지 비장 임파구에 대한 면역 반응을 조사하였다. 복분자의 70% 에틸 알코올 추출물을 돼지 비장 임파구에 처리하였다. 복분자의 추출물은 비장 면역세포의 증식을 촉진하였으며, 복분자(라즈베리)의 추출물은 돼지 비장 임파구에 대해 CD3-T 세포, CD4-T 세포와 B 세포의 구성분을 증가시켰다. 복분자 추출물은 비장 임파구 세포의 활력을 증가시킴으로써 면역반응을 향상시켰다. 본 연구에서 우리는 돼지 비장세포에 복분자 추출물을 처리한 결과 8개의 유전자 발현이 증가되었음을 확인했는데, 이들 중에는 세포구조와 면역반응에 관여하는 유전자가 포함되었다. 특히 microtubule-associated protein 4, cytoplasmic dynein heavy chain, tumor necrosis factor alpha, 및 lymphotoxin-beta receptor precursor 유전자 발현이 증가되었다. 한편 10개의 유전자는 복분자 추출물에 의해 그 발현이 감소되었다.

Keywords

References

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