Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Effects of Gyejijakyakjimo-tang extract on inhibition of PGE2 synthesis and NO production in murine raw 264.7 macrophage cells

  • Park, Kyoung-Su (Department of Rehabilitation, College of Oriental Medicine, Kyung Hee University) ;
  • Hwang, Mi-Ja (Department of Rehabilitation, College of Oriental Medicine, Kyung Hee University) ;
  • Nam, Ki-Bong (Department of Rehabilitation, College of Oriental Medicine, Kyung Hee University) ;
  • Ryu, Ji-Mi (Department of Rehabilitation, College of Oriental Medicine, Kyung Hee University) ;
  • Chung, Seok-Hee (Department of Rehabilitation, College of Oriental Medicine, Kyung Hee University)
  • Published : 2008.02.29
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Abstract

Gyejijakyakjimo-tang is a multi-herbal formula that is composed of nine medicinal herbs. Gyejijakyakjimo-tang has been reported to have antipyretic and analgesic effects. Gyejijakyakjimo-tang has traditionally been used for goat and rheumatoid arthritis. However, analgesic and antiinflammatory effects of Gyejijakyakjimo-tang has not been clarified yet. In this study, we investigated the analgesic and anti-inflammatory effect of the aqueous extract of Gyejijakyakjimo-tang. We evaluated the aqueous extract of Gyejijakyakjimo-tang on Lipopolysaccharide (LPS)-induced inflammation in murine raw 264.7 macrophage cells. For this study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), prostaglandin $E_2$ ($PGE_2$) immunoassay, and nitric oxide (NO) detection were performed. Gyejijakyakjimo-tang suppressed $PGE_2$ synthesis and NO production by inhibiting the LPS-induced expressions of COX-2 and iNOS mRNA in murine raw 264.7 macrophage cells. These results show that Gyejijakyakjimo-tang has the analgesic and anti-inflammatory effect by mostly suppressing COX-2 and iNOS expressions, and resulting in the inhibition of $PGE_2$ synthesis and NO production.

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References

  1. Ahn KS, Noh EJ, Zhao HL, Jung SH, Kang SS, Kim YS. (2005) Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-κB activation in raw 264.7 cells. Life Sci. 76, 2315-2328 https://doi.org/10.1016/j.lfs.2004.10.042
  2. Bansky D, Barolet R. (1990) Chinese Herbal Medicine Formulas and Strategies. Eastland Press Seattle. 7-8
  3. Bredt DS, Snyder SH. (1994) Nitric oxide: a physiologic messenger molecule. Annu. Rev. Biochem. 63, 175-195 https://doi.org/10.1146/annurev.bi.63.070194.001135
  4. Cuzzocrea S, Chatterjee PK, Mazzon E, McDonald MC, Dugo L, Di Paola R, Serraino I, Britti D, Caputi AP, Thiemermann C. (2002) Beneficial effects of GW274150, a novel, potent and selective inhibitor of iNOS activity, in a rodent model of collageninduced arthritis. Eur. J. Pharmacol. 453, 119-129 https://doi.org/10.1016/S0014-2999(02)02338-5
  5. Dickerson C, Undem B, Bullock B, Winchurch RA. (1998) Neuropeptide regulation of proinflammatory cytokine response. J. Leukoc. Biol. 63, 602-605
  6. Guo Q. (2006) Obervation of Change ESR and CRP in treatment of Feng-shi(風濕) disease by Guizhishaoyaozhimu decoction. J. of Gansuzhongyi (甘肅中醫). 19, 20-21
  7. Jaffrey SR, Snyder SH. (1995) Nitric oxide: a neural messenger. Annu. Rev. Cell Dev. Biol. 11, 417-440 https://doi.org/10.1146/annurev.cb.11.110195.002221
  8. Jang MH, Lim S, Han SM, Park HJ, Shin I, Kim JW, Kim MJ, Lee JS, Kim KA, Kim CJ. (2003) Harpagophytum procumbens suppresses lipopolysaccharide-stimulated expressions of cyclooxygenase-2 and inducible nitric oxide synthase in fibroblast cell line L929. J. Pharmacol. Sci. 93, 367-371 https://doi.org/10.1254/jphs.93.367
  9. Kim HW, Kwon YB, Ham TW, Roh DH, Yoon SY, Han HJ, Kang SK, Lee HJ, Mar WC, Yang IS, Beitz AJ, Lee JH. (2002) The antinociceptive and antiinflammatory effect of ethylacetate extracts from Bang-Poong (Radix ledebouriellae) on the Freund's adjuvant-induced arthritis in rats. J. Vet. Sci. 3, 343-349
  10. Kim S, Kim H, Choi JW, Lee CK. (1995) Antiinflammatory, analgesic and antihyperuricemic effects of Gyejijakyakjimo-tang in rats. Kor. J. Pharmacogn. 26, 66-73
  11. Kim SJ, Jeong HJ, Moon PD, Myung NY, Kim MC, Kang TH, Lee KM, Park RK, So HS, Kim EC, An NH, Um JY, Kim HM. (2007) The COX-2 inhibitor SC-236 exerts anti-inflammatory effects by suppressing phosphorylation of ERK in a murine model. Life Sci. 81, 863-872 https://doi.org/10.1016/j.lfs.2007.06.027
  12. Kubes P, McCafferty DM. (2000) Nitric oxide and intestinal inflammation. Am. J. Med. 109, 150-158 https://doi.org/10.1016/S0002-9343(00)00480-0
  13. Lee IS, Shin HD. (1990) An Experimental Studies on the Efficacy of Geyjijakkarkjimotang. Master's thesis of Kyung Hee University Grauate school. 1-27
  14. Lee SH, Soyoola E, Chanmugam P, Hart S, Sun W, Zhong H, Liou S, Simmons D, Hwang D. (1992) Selective expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide. J. Biol. Chem. 267, 25934-25938
  15. Lee SJ, Shin EJ, Son KH, Chang HW, Kang SS, Kim HP. (1995) Anti-inflammatory activity of the major constituents of Lonicera japonica. Arch. Pharm. Res. 18, 133-135
  16. Li CQ, He LC, Jin JQ. (2007) Atractylenolide I and atractylenolide III inhibit Lipopolysaccharide -induced TNF-$\alpha$ and NO production in macrophages. Phytother. Res. 21, 347-353 https://doi.org/10.1002/ptr.2040
  17. Li K. (1989) Jinguiyaolue(金要略). beijing(北京): ren-min-wei-sheng-chu-ban-she(人民衛生-出版社). 140-141
  18. MacMicking J, Xie QW, Nathan C. (1997) Nitric oxide and macrophage function. Annu. Rev. Immunol. 15, 323-350 https://doi.org/10.1146/annurev.immunol.15.1.323
  19. McGeer PL, McGeer EG. (1995) The inflammatory response system of brain: implications for therapy of Alzheimer and other neurodegenerative diseases. Brain Res. Brain Res. Rev. 21, 195-218 https://doi.org/10.1016/0165-0173(95)00011-9
  20. Moalem G, Tracey DJ. (2006) Immune and inflammatory mechanisms in neuropathic pain. Brain Res Rev 51, 240-264 https://doi.org/10.1016/j.brainresrev.2005.11.004
  21. Moncada S, Palmer RM, Higgs EA. (1991) Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol. Rev. 43, 109-142
  22. Myers LK, Kang AH, Postlethwaite AE, Rosloniec EF, Morham SG, Shlopov BV, Goorha S, Ballou LR. (2000) The genetic ablation of cyclooxygenase 2 prevents the development of autoimmune arthritis. Arthritis. Rheum. 43, 2687-2693 https://doi.org/10.1002/1529-0131(200012)43:12<2687::AID-ANR8>3.0.CO;2-9
  23. Nishizawa M, Sutherland WH, Nukada H. (1995) Gosha-jinki-gan (herbal medicine) in streptozocininduced diabetic neuropathy. J. Neurol. Sci. 132,177- 181 https://doi.org/10.1016/0022-510X(95)00141-N
  24. Oh MS, Huh Y, Bae H, Ahn D, Park SK. (2005) The multi-herbal formula Guibi-tang enhances memory and increases cell proliferation in the rat hippocampus. Neurosci. Lett. 379, 205-208 https://doi.org/10.1016/j.neulet.2004.12.077
  25. Ohshima H, Bartsch H. (1994) Chronic infections and inflammatory processes as cancer risk factors: possible role of nitric oxide in carcinogenesis. Mutat. Res. 305, 253-264 https://doi.org/10.1016/0027-5107(94)90245-3
  26. Omiya Y, Goto K, Suzuki Y, Ishige A, Komatsu Y. (1999) Analgesia-producing mechanism of processed Aconiti tuber: role of dynorphin, an endogenous κ-opioid ligand, in the rodent spinal cord. Jpn. J. Pharmacol. 79, 295-301 https://doi.org/10.1254/jjp.79.295
  27. Park HJ, Kim IT, Won JH, Jeong SH, Park EY, Nam JH, Choi J, Lee KT. (2007) Anti-inflammatory activities of ent-16αH,17-hydroxy-kauran-19-oic acid isolated from the roots of Siegesbeckia pubescens are due to the inhibition of iNOS and COX-2 expression in RAW 264.7 macrophages via NF-κB inactivation. Eur. J. Pharmacol. 558, 185-193 https://doi.org/10.1016/j.ejphar.2006.11.036
  28. Shao J, Sheng H, Inoue H, Morrow JD, DuBois RN. (2000) Regulation of constitutive cyclooxygenase-2 expression in colon carcinoma cells. J. Biol. Chem. 275, 33951-33956 https://doi.org/10.1074/jbc.M002324200
  29. Srivastava KC, Mustafa T. (1992) Ginger (Zingiber officinale) in rheumatism and Musculo- skeletal disorders. Med. Hypotheses 39, 342-348 https://doi.org/10.1016/0306-9877(92)90059-L
  30. Surh YJ, Chun KS, Cha HH, Han SS, Keum YS, Park KK, Lee SS. (2001) Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-eB activation. Mutat. Res. 480-481, 243-268 https://doi.org/10.1016/S0027-5107(01)00183-X
  31. Szabo C. (1995) Alterations in nitric oxide production in various forms of circulatory shock. New Horiz. 3, 2-32
  32. Talhouk RS, Karam C, Fostok S, El-Jouni W, Barbour EK. (2007) Anti-inflammatory bioactivities in plant extracts. J. Med. Food 10, 1-10 https://doi.org/10.1089/jmf.2005.055
  33. Yue H, Yuan LG, Chen XJ, Meng M. (2005) Effect of TNF-${\alpha}$ and IL-1${\beta}$ at type 2 collagen induced arthritis mice by Guizhishaoyaozhimu decoction. J. Zhong Guo Zhong Yi Yao Xin Xi. 12, 27-28
  34. Yui Y, Hattori R, Kosuga K, Eizawa H, Hiki K, Kawai C. (1991) Purification of nitric oxide synthase from rat macrophages. J. Biol. Chem. 266, 12544-12547
  35. Zhang Q. (2005) Effect of Inhibition CRP, IL-2 at collagen induced arthritis mice by Guizhishaoyaozhimu decoction. J. TCM. 46, 854-856
  36. Zhang Q. (2006) Effect of Inhibition T lymphocyte at collagen induced arthritis mice by Guizhishaoyaozhimu decoction. Journal of Chengdu University of TCM. 29, 24-26
  37. Zheng YQ, Wei W, Zhu L, Liu JX. (2007) Effects and mechanisms of Paeoniflorin, a bioactive glucoside from paeony root, on adjuvant arthritis in rats. Inflamm. Res. 56, 182-188