Molecular & Cellular Toxicology

  • 제4권1호
  • /
  • Pages.85-91
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  • 2008
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  • 1738-642X(pISSN)
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  • 2092-8467(eISSN)
대한독성유전 단백체학회 (The Korean Society of Toxicogenomics and Toxicoproteomics)
권호 표지

Effects of EGb 761 and Korean Red Ginseng on Melanogenesis in B16F10 Melanoma Cells and Protection Against UVB Irradiation in Murine Skin

  • Han, Seon-Kyu (Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology) ;
  • Choi, Wook-Hee (Department of Health Science, Dongduk women's University) ;
  • Ann, Hyoung-Soo (Department of Pharmacology, Dongduk women's University) ;
  • Ahn, Ryoung-Me (Department of Health Science, Dongduk women's University) ;
  • Yi, Seh-Yoon (College of Natural Science, Suwon University)
  • 발행 : 2008.03.31
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초록

These days there is a constant possibility of exposure to UV radiation which can cause abnormal production of melanin and result in skin disease such as hyperpigmentation and melanoma. Many materials were investigated for skin whitening and protection against UV radiation. In this study, we assessed the melanogenesis inhibitory activities of Korean Red Ginseng (KRG, Ginseng Radix Rubra) and Ginkgo (EGb 761 Ginkgo Biloba) in an attempt to develop a new skin whitening agent derived from natural products. B16F10 melanoma cells were treated for 48 hr with KRG and EGb 761. The inhibitory effect on melanogenesis was measured and related cytokines and proteins expression were also investigated by RT-PCR and Western blotting. In addition, we also assessed the effects of these substances on the skin of C57BL/6 mice. Cell growth, melanin content and tyrosinase activity were inhibited effectively in B16F10 melanoma cells treated with KRG and EGb 761. Moreover, tyrosinase mRNA expression was inhibited clearly and melanogenesis related proteins (MRPs) containing tyrosinase, TRP1 and TRP2 were also reduced by KRG and EGb761, while cytokines such as IL-$1{\beta}$ and IL-6 were induced. In the case of UV irradiated mice, we observed induction of cytokine mRNA levels and reduction of MRPs mRNA expression. In addition, a decrease in pigmentation from treatment with KRG and EGb 761 on the skin of mice was observed. These results indicate that KRG and EGb 761 inhibit melanogenesis in B16F10 cells and have display protective activities against UVB. Therefore, we suggest that KRG and EGb 761 are good candidates to be used as whitening agents and UVB protectors for the skin.

키워드

참고문헌

  1. Hearing, V. J. Biochemical control of melanogenesis and mealnosomal organization. J Investigative Dermatol Symp Proc 4:24-28 (1999)
  2. Hearing, V. J. & Jimenez, M. Mammalian tyrosinasethe critical regulatory control point in melanocyte pigmentation. Int J Biochem 19:1141-1147 (1987) https://doi.org/10.1016/0020-711X(87)90095-4
  3. Kobayashi, T. et al. Tyrosinase related protein 1 (TRP-1) functions as a DHICA oxidase in melanin biosynthesis. EMBO J 13:5818-5825 (1994)
  4. Yokoyama, K., Yasumoto, K., Suzuki, H. & Shibahara, S. Cloning of the human DOPAchrome tautomerase/tyrosinase-related protein 2 gene andidentification of two regulatory regions required for its pigment cell-specific expression. J Biol Chem 269:27080-27087 (1994)
  5. Matsunaga, H. et al. Studies on the panaxytriol of Panax ginseng C. A. Meyer.; Isolation, determination and antitumor activity. Chem Pham Bull 37:1279-1281 (1989) https://doi.org/10.1248/cpb.37.1279
  6. Lyn, S. Y. & Chnag, H. P. Induction of superoxide dismutase and catalase activity in different rat tissues and protection from UVB irradiation after topical application of Ginkgo biloba extracts. Methods Find Exp Clin Pharmacol 19:367-371 (1997)
  7. Kwak, W. J. et al. Effects of Ginkgetin from Ginkgo biloba leaves on cyclooxygenases and in vivo skin inflammation. Planta med 68:316-321 (2002) https://doi.org/10.1055/s-2002-26742
  8. Im, S. J. et al. Effect of radix ginseng and radix trichosanthis on the melanogenesis. Biol Pharm Bull 26:849-853 (2003) https://doi.org/10.1248/bpb.26.849
  9. Ozkur, M. K. et al. The effects of EGb 761 on lipid peroxide levels and superoxide dismutase activity in sunburn. Photodermatol Photoimmunol Photomed 18:117-120 (2002) https://doi.org/10.1034/j.1600-0781.2002.00727.x
  10. Kameyama, K. et al. Pigment production in murine melanoma cells is regulated by tyrosinase, tyrosinaserelated protein 1 (TRP1), DOPAchrome tautomerase (TRP2), and a melanogenic inhibitor. J Invest Dermatol 100:126-131 (1993) https://doi.org/10.1111/1523-1747.ep12462778
  11. Boissy, R. E. & Nordlund, J. J. Molecular basis of congenital hypopigmentary disorders in humans: a review. Pigment Cell Res 10:12-24 (1997) https://doi.org/10.1111/j.1600-0749.1997.tb00461.x
  12. Mallick, S. et al. Human placentallipid induces melanogenesis by increasing the expression of tyrosinase and its related proteins in vitro. Pigment Cell Res 18:25-33 (2005) https://doi.org/10.1111/j.1600-0749.2004.00193.x
  13. Jimbow, K., Obata, H., Pathak, M. A. & Fitzpatrick, T. B. Mechanism of depigmentation by hydroquinone. J Invest Dermatol 62:436-449 (1974) https://doi.org/10.1111/1523-1747.ep12701679
  14. Imokawa, G. & Mishinma, Y. Loss of melanogenic properties in tyrosinases induced by glucosylation inhibitors within malignant melanoma cells. Cancer Res 42:1994-2002 (1982)
  15. Maeda, K. & Fukuda, M. Arbutin: mechanism of its depigmenting action in human melanocyte culture. J Pharmacol Exp Ther 276:765-769 (1996)
  16. Roh, J. S., Han, J. Y., Kim, J. H. & Hwang, J. K. Inhibitory effects of active compounds isolated from safflower (Carthamus tinctorius L.) seeds for melanogenesis. Biol Pharm Bull 27:1976-1978 (2004) https://doi.org/10.1248/bpb.27.1976
  17. Morelli, J. G. & Norris, D. A. Influence of inflammatory mediators and cytokines on human melanocyte function. J Invest Dermatol 100:191S-195S (1993) https://doi.org/10.1111/1523-1747.ep12465168
  18. Swope, V. B., Abdel-Malek, Z., Kassem, L. M. & Nordlund, J. J. Interleukin 1$\alpha$ and 6 and tumor necrosis factor-$\alpha$ are paracrine inhibitors of human melaocyte proliferation and melanogenesis. J Invest Dermatol 96:180-185 (1991) https://doi.org/10.1111/1523-1747.ep12460991
  19. Nakajima, S. et al. The effects of ginseng radix rubra on human vascular endothelial cells. Am J Chin Med 26:365-373 (1998) https://doi.org/10.1142/S0192415X98000403
  20. Martinez-Esparza, M. et al. Mechanisms of melanogenesis inhibition by tumor necrosis factor-alpha in B16/F10 mouse melanoma cells. Eur J Biochem 255:139-146 (1998) https://doi.org/10.1046/j.1432-1327.1998.2550139.x
  21. Lee, S. K. et al. Prevention of ultraviolet radiationinduced suppression of contact hypersensitivity by Aloe vera gel components. International Journal of immunopharmacology 21:303-310 (1999) https://doi.org/10.1016/S0192-0561(99)00012-0
  22. Curto, E. V. et al. Inhibitors of mammalian melanocyte tyrosinase: In vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors. Biochem Pahrmacol 57:663-672 (1999) https://doi.org/10.1016/S0006-2952(98)00340-2
  23. Tomita, Y., Maeda, K. & Tagami, H. Melanocyte-stimulating properties of arachidonic acid metabolites: possible role in postinflammatory pigmentation. Pigment Cell Res 5:357-361 (1992) https://doi.org/10.1111/j.1600-0749.1992.tb00562.x