Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Morphological and Phylogenetic Characteristics of a Nematophagous Fungus, Drechslerella brochopaga Kan-23

국내 미기록종 선충포식성 곰팡이 Drechslerella brochopaga Kan-23의 형태 및 계통분류

  • 조천휘 ((주)카프코 생물화학연구소) ;
  • 강두선 ((주)카프코 생물화학연구소) ;
  • 김윤지 (목원대학교 미생물생태자원연구소) ;
  • 황경숙 (목원대학교 미생물생태자원연구소)
  • Published : 2008.03.31
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Abstract

Strain Kan-23 was extracted from nematophagous fungi, which were isolated from the soil sample of oriental melon field. The strain exhibited the slow-growing characteristic forming conidia after prolonged incubation for 30 days. Morphological features of strain Kan-23 were observed under scanning electron microscope (SEM). It possesses erect conidiophores which contain $2{\sim}3$ side branches, with each branch producing $5{\sim}10$ conidia. The size of conidiophores were between $160{\sim}450\;{\mu}m$. Conidia were ellipsoidal with three septa[septum] in each conidium. Strain Kan-23 captured nematodes by means of giant constricting rings, which were observed in the glucose peptone agar medium. ITS region of rDNA sequence was analyzed. On the basis of the high sequence similarity of ITS region (99%), the Kan-23 strain was closely related to Drechslerella brochopaga (U51950). This is the first report on Drechslerella brochopaga as a nematophagous fungus in Korea.

참외 경작지 토양으로부터 순수 분리된 선충포식성 곰팡이 Kan-23 균주는 배양 30일 이후에 분생포자가 형성되는 증식이 매우 더딘 특성을 나타내었다. 주사전자현미경(SEM)을 이용하여 Kan-23 균주의 미세형태 구조를 관찰한 결과 분생포자병의 각 가지마다 $5{\sim}10$개의 분생포자를 형성하였으며, 분생포자는 3개의 격막을 형성하고 타원형을 나타내었고, 수축성 고리형태(constricting ring)의 포식 구조체를 형성하는 특징을 나타내었다. 다양한 배지를 이용하여 포식 구조체의 형태학적 특성을 비교 검토한 결과 GPA 배지에서 2배 이상 크기의 대형 수축성 고리 형태의 포식 구조체를 형성하는 특징을 나타내었다. Kan-23 균주의 rDNA의 ITS 영역의 염기서열을 분석한 결과, Drechslerella 속의 계통군에 속하였으며, 특히 Drechslerella brochopaga (U51950)와 99%의 높은 염기서열 유사도를 나타내어 Drechslerella brochopaga로 동정되어 국내 미기록종임이 확인되었다.

Keywords

References

  1. Ahrn, D., B.M. Ursing, and A. Tunlid. 1998. Phylogeny of nematode-trapping fungi based on 18S rDNA sequences. FEMS Microbiol. Lett. 158, 179-184 https://doi.org/10.1111/j.1574-6968.1998.tb12817.x
  2. Barron, G.L. 1977. The nematode trapping fungi, p. 140. Canadian Biological Publications, Guelph, Ontario, Canada
  3. Cho, C.W., D.S. Kang, H.K. Jeon, H.S. Son, and K.S. Whang. 2007. Morphological and phylogenetic characteristics of nematophagous fungi. J. Kor. Soc. Appl. Biol. Chem. 50, 101-106
  4. Cooke, R.C. and B.E.S. Godfrey. 1964. A key to the nematode-destroying fungi. Trans. Brit. Mycol. Soc. 47, 61-74 https://doi.org/10.1016/S0007-1536(64)80081-4
  5. Fresenius, G. 1852. Beitr. Mykol. Heft. No. 1-2, p. 1-80
  6. Haard, K. 1968. Taxonomic studies on the genus Arthrobotrys Corda. Mycologia 60, 1140-1159 https://doi.org/10.2307/3757214
  7. Kim, D.G. 1997. Nematophagous fungi. KSM Newsletter 9, 29-46
  8. Kim, D.G., S.G. Bae, and Y.S. Shin. 2001. Distribution of nematophagous fungi under different habitats. Kor. J. Microbiol. 29, 123-126
  9. Kim, D.G., J.K. Lee, Y.G. Lee, Y.C. Choi, and Y.K. Kim. 1997. Description of five species of Arthrobotrys (Corda) Schenck, Kendrick & Pramer in Korea and Their Key. RDA. J. Crop Protec. 39, 33-41
  10. Li, Y., R. Jeewon, K.D. Hyde, M.H. Mo, and K.Q. Zhang. 2006. Two new species of nematode-trapping fungi: relationships inferred from morphology, rDNA and protein gene sequence analyses. Mycol. Res. 110, 790-800 https://doi.org/10.1016/j.mycres.2006.04.011
  11. Liou, G. Y. and S.S. Tzean. 1997. Phylogeny of the genus Arthrobotrys and allied nematode-trapping fungi based on rDNA sequences. Mycologia 89, 876-884 https://doi.org/10.2307/3761108
  12. Meyer, S.L.F., L.K. Carta, and S.A. Rehner. 2005. Morphological variability and molecular phylogeny of the nematophagous fungus Monacrosporium drechsleri. Mycologia 97, 405-415 https://doi.org/10.3852/mycologia.97.2.405
  13. Nordbring-Hertz, B., H.B. Jansson, and A. Tunlid. 2006. Nematophagous Fungi. In Encyclopedia of life sciences, John Wiley & Sons, Ltd: Chichester. [doi:10.1038/npg.els.0004293]
  14. Park, H.C., S.M. Kim, Y.C. Kim, C.Y. Lee, and I.S. Choi. 1997. Isolation and morphological characteristics of nematophagous fungi. J. Agri. Tech. Dev. Inst. 1, 77-83
  15. Park, Y.K. and Y.N. Lee. 1983. Isolation and identification of nematode-trapping fungi. Kor. J. Microbiol. 21, 127-134
  16. Pramer, D. and E.L. Schmidt. 1964. Experimental soil microbiology. p. 20-21. Burgess. Minneapolis, USA
  17. Rifai, M.A. and R.C. Cooke. 1966. Studies on some didymosporous genera of nematode-trapping hyphomycetes. Trans. Brit. Mycol. Soc. 49, 147-168 https://doi.org/10.1016/S0007-1536(66)80045-1
  18. Saccardo, P.A. 1886. Sylloge fungorum omnium hucusque cognitorum, 4th ed. Privately published, Padova, Italy
  19. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstruction phylogenetic trees. Mol. Biol. Evol. 4, 281-295
  20. Scholler, M., G. Hagedorn, and A. Rubner. 1999. A reevaluation of predatory orbiliaceous fungi. II. A new generic concept. Sydowia 51, 99
  21. Soprunov, F.F. 1958. Predacious hyphomycetes and their application in the control of pathogenic nematodes, p. 1-365. Academy of science of the turkmen SSR. Jerusalem, Israel
  22. Susan, L.F.M., L.K. Carta, and S.A. Rehner. 2005. Morphological variability and molecular phylogeny of the nematophagous fungus Monacrosporium drechsleri. Mycologia 97, 405-415 https://doi.org/10.3852/mycologia.97.2.405
  23. Thomson, J.D., D.G. Higgins, and T.J. Gibson. 1994. CLUSTAL W;improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680 https://doi.org/10.1093/nar/22.22.4673
  24. Turnbull, J.R. and K. Zachariah. 1978. The induction of giant ring traps and regulation of conidiogenesis in the predacious fungus Dactylella brochopaga. Can. J. Microbiol. 24, 1182-1189 https://doi.org/10.1139/m78-192
  25. Yang, Y. and Z.L. Xing. 2006. A new generic approach to the taxonomy of predatory anamorphic Orbiliaceae (Ascomycotina). Mycotaxon 97, 153-161
  26. Yu, Z.-F., Y. Zhang, M. Qiao, H.-O. Baral, E. Weber, and K. Zhang. 2006. Drechslerella brochopaga, the anamorph of Orbilia (Hyalinia) orientalis. Mycotaxon 96, 163-168
  27. Zachariah, K. and J.P. Insell. 1979. The nuclei of the giant traps of a mutant of Monacrosporium bembicodes (Drechsler) Subram (Dactylella brochopaga Drechsler). Antonie Van Leeuwenhoek 45, 141-147 https://doi.org/10.1007/BF00400787
  28. Zhu, H., F. Qu, and L.H. Zhu. 1993. Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride. Nucleic Acids Res. 21, 5279-5280 https://doi.org/10.1093/nar/21.22.5279