Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Optimization of Bisphenol A Biodegradation by Trametes versicolor

Trametes versicolor에 의한 Bisphenol A 생분해의 최적조건

  • Kang, Ae-Ri (Division of Life Sciences, and Research Institute of Life Sciences, Kangwon National University) ;
  • Choi, Hyoung-Tae (Division of Life Sciences, and Research Institute of Life Sciences, Kangwon National University) ;
  • Song, Hong-Gyu (Division of Life Sciences, and Research Institute of Life Sciences, Kangwon National University)
  • 강애리 (강원대학교 자연과학대학 생명과학부, 강원대학교 생명과학연구소) ;
  • 최형태 (강원대학교 자연과학대학 생명과학부, 강원대학교 생명과학연구소) ;
  • 송홍규 (강원대학교 자연과학대학 생명과학부, 강원대학교 생명과학연구소)
  • Published : 2008.03.31
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Abstract

Optimal conditions for the biodegradation of endocrine-disrupting bisphenol A (BPA) were examined for the white rot fungus Trametes versicolor isolated in Korea. T. versicolor degraded 100% of 50 mg/L bisphenol A during 12 hr in yeast extract-malt extract-glucose (YMG) medium. When BPA was added to the 5-day preincubated fungal culture in YMG medium, all BPA was removed in 2 hr. T. versicolor could efficiently degrade BPA at $35^{\circ}C$, pH 6 in YMG medium. T. versicolor could more easily remove BPA of $1{\sim}25\;mg/L$ than that of higher concentrations ($50{\sim}100\;mg/L$) in YMG medium. T. versicolor degraded 100% of 50 mg/L BPA for 36 h in a minimal medium, which is lower degradation rate than that in YMG medium. Optimal conditions for BPA biodegradation in the minimal medium were similar to those in YMG medium. When BPA (50 mg/L) was added into domestic wastewater, it could be completely removed by T. versicolor. During the biodegradation of BPA by T. versicolor in YMG medium, its estrogenic activity decreased.

국내에서 분리된 구름버섯(Trametes versicolor)에 의한 내분비계 장애물질 bisphenol A의 생분해 효율을 높이기 위하여 그 생분해 최적조건을 조사하였다. T. versicolor는 Yeast extract-Malt extract-Glucose (YMG) 배지에서 12시간 이내에 50 mg/L bisphenol A를 100% 생분해하며, 5일 동안 선배양 시에는 2시간 이내에 100% 제거하였다. Bisphenol A의 농도가 낮을수록, 온도는 $35^{\circ}C$, pH 6에서 생분해율이 가장 높았으며 교반의 영향은 크지 않았다. 최소배지에서는 생분해율이 약간 낮았지만 최적조건은 YMG 배지에서와 유사하였다. 생분해에 관여하는 리그닌 분해효소 laccase의 mediator인 ABTS [2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt]를 최소배지에 첨가할 경우 bisphenol A의 생분해가 촉진되었다. 접종물 중 배양 상등액에 대한 균체량의 비율이 높을수록 생분해율이 증가하였는데 그 비율이 50%일 때 폐수에 첨가한 50 mg/L의 bisphenol A가 4일째에 100% 분해되었다. YMG 배지에서 bisphenol A의 생분해에 따라 내분비계 장애효과를 나타내는 에스트로겐 활성은 감소하였다.

Keywords

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