Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Effect of Aerva lanata against oxalate mediated free radical toxicity in urolithiasis

  • Published : 2008.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was undertaken to evaluate the antioxidant potential of A. lanata on oxalate mediated free radical toxicity in ethylene glycol induced calcium oxalate urolithic rats. Calcium oxalate (CaOX) stone was induced by 0.75% ethylene glycol in drinking water for 28 days. From $29^{th}$ day onwards, the CaOX urolithic rats were treated with A. lanata aqueous suspension (2,000 mg/kg body weight/dose/day) orally for another 28 days. At the end of experimental periods the animals were sacrificed, samples were collected and analyzed the lipid peroxidation product, protein oxidation product, enzymatic and non-enzymatic antioxidants in normal and experimental groups. Lipid peroxidation and protein oxidation products were significantly elevated while enzymatic and non-enzymatic antioxidant levels were significantly decreased in ethylene glycol induced CaOX urolithic rats when compared with control rats. The above alterations were reverted to near control in rats treated with aqueous suspension of A. lanata. This study suggests that A. lanata could prevent the free radical formation from calcium oxalate urolithiasis in rats and protecting the renal cells from oxidative injury.

Keywords

References

  1. Afaq SH, Tajuddin S, Afridi R. (1991) Bisheri Booti (Aerva lanata) some lesser known uses and pharmacognosy. Ethnobotany. 5, 37-40
  2. Anand R, Patnaik GK, Kulshreshtha DK, Dhawan BN. (1992) Antiurolitiatic and diuretic activity of Lupeol, the active constituent isolated from Crataeva nurvala (Buch Ham). Indian J. Pharmacol. 24, 48-49
  3. Baker H, Frank O, De Angeles B, Feinglod S. (1980) Plasma tocopherol in man at various times after ingesting free or acetylated tocopherol. Nutr. Rep. Int. 21, 531-537
  4. Beuge JA, Aust SD. (1978) The thiobarbituric acid assay. Meth. Enzymol. 52, 306-307
  5. Castilla P, Echarri R, Davalos A, Cerrato F, Ortega H, Teruel JL, Lucas MF, Gomez-Coronado D, Ortuno J, Lasuncion MA. (2006) Concentrated red grape juice exert antioxidant, hypolipidemic, and anti-inflammatory effects in both hemodialysis patients and healthy subjects. Am. J. Clin. Nutr. 84, 252-262
  6. Chandra S, Sastry MS. (1990) Chemical constituents of Aerva lanata. Fitoterapia 61, 188-190
  7. Chowdhury D, Sayeed A, Islam A, Bhuiyan MSA, Khan GRMAM. (2002) Antimicrobial activity and cytotoxicity of Aerva lanata. Fitoterapia 73, 92-94 https://doi.org/10.1016/S0367-326X(01)00369-0
  8. Cross CE, Halliwell B, Borish ET, Pryor W, Ames BN, Saul RL, McCord JM, Harman D. (1987) Oxygen radicals and human disease. Ann. Intern. Med. 107, 526-545
  9. Grases F, Garcia-Ferragut L, Costa-Bauza A. (1998) Development of calcium oxalate crystals on urolithium: effect of free radicals. Nephron 78, 296-301 https://doi.org/10.1159/000044939
  10. Habig WH, Pabst MJ, Jakpoby WB. (1974) GST, a first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130-7139
  11. Halliwell B. (1994) Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 344, 721-724 https://doi.org/10.1016/S0140-6736(94)92211-X
  12. Huang HS, Ma MC, Chen CF, Chen J. (2003) Lipid peroxidations and its correlations with urinary levels of oxalate, citric acid, and osteopontin in patients with renal calcium oxalate stones. Urology 62, 1123-1128 https://doi.org/10.1016/S0090-4295(03)00764-7
  13. Kakkar P, Das B, Viswanathan PN. (1984) A modified spectrophotometric assay of SOD. Indian J. Biochem. Biophys. 21, 130-132
  14. Khan SR. (1995) Animal model of calcium oxalate nephrolithiasis. In: Caicium oxalate in biological systems, edited by Khan SR, pp. 343-359, CRC Press. Boca Raton, FL
  15. Korenberg A, Horecker BL, Horecker BL, Smyrniotis PZ. (1955) Glucose-6-phosphate dehydrogenase 6-phospho-gluconic dehydrogenase. Meth. Enzymol. 1, 323-327 https://doi.org/10.1016/0076-6879(55)01046-X
  16. Levine RL, Garland D, Oliver CN, Amici A, Lenz AZ, Ahn B, Shaltiel S, Stadtman ER. (1990) Assay of carbonyl in protein. Meth. Enzymol. 186, 464-478
  17. Lewandowski S, Rodgers AL. (2004) Idiopathic calcium oxalate urolithiasis: risk factors and conservative treatment. Clin. Chim. Acta 345, 17-34 https://doi.org/10.1016/j.cccn.2004.03.009
  18. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. (1951) Protein measurement with the Folin's reagent. J. Biol. Chem. 193, 265-276
  19. Malini MM, Lenin M, Varalakshmi P. (2000) Protective effect of triterpenes on calcium oxalate crystal-induced peroxidative changes in experimental urolithiasis. Pharmacol. Res. 41, 413-418 https://doi.org/10.1006/phrs.1999.0601
  20. Moron MS, Defierre JW, Mannervik B. (1979) Levels of glutathione, glutathione reductase, glutathiones-transferase activities in rat lung and liver. Biochim. Biophys. Acta 582, 67-78
  21. Muzakova V, Kandar R, Vojtisek P, Skalicky J, Vankova R, Cegan A, Cervinkova Z. (2001) Antioxidant vitamin levels and glutathione peroxidase activity during ischemia/reperfusion in myocardial infarction. Physiol. Res. 50, 389-396
  22. Nevin KG, Vijayammal PL. (2003) Effect of Aerva lanata on solid tumor induced by DLA cells in mice. Fitoterapia 74, 578-582 https://doi.org/10.1016/S0367-326X(03)00148-5
  23. Omaye ST, Tumball JD, Sauberlich HE. (1979) Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. Meth. Enzymol. 62, 1-11
  24. Raghavan B, Kumari SK. (2006) Effect of Terminalia arjuna stem bark on antioxidant status in liver and kidney of alloxan diabetic rats. Indian J. Physiol. Pharmacol. 50, 133-142
  25. Rajeswari A, Varalakshmi P. (2006) Low molecular weight heparin protection against oxalate-induced oxidative renal insult. Clin. Chim. Acta 370, 108-114 https://doi.org/10.1016/j.cca.2006.01.027
  26. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. (1973) Selenium: biochemical roles as component of glutathione peroxidase. Science 179, 588-590 https://doi.org/10.1126/science.179.4073.588
  27. Selvam R, Bijikurien T. (1987) Induction of lipid peroxidation by oxalate in experimental rat urolithiasis. J. Biosci. 12, 367-373 https://doi.org/10.1007/BF02898585
  28. Selvam R, Bijikurien T. (1991) Methionine feeding prevents kidney stone deposition by restoration of free radical mediated changes in experimental rat urolithiasis. J. Nutr. Biochem. 2, 644-651 https://doi.org/10.1016/0955-2863(91)90062-A
  29. Sen CK, Rahkila P, Hanninen O. (1993) Glutathione metabolism in skeletal muscle derived cells of the L6 line. Acta Physiol. Scand. 148, 21-26
  30. Sinha AK. (1972) Colorimetric assay of catalase. Anal. Biochem. 47, 389-394 https://doi.org/10.1016/0003-2697(72)90132-7
  31. Soundararajan P, Mahesh R, Ramesh T, Hazeena Begum V. (2006) Effect of Aerva lanata on calcium oxalate urolithiasis in rats. Indian J. Exp. Biol. 44, 981-986
  32. Srinivasan S, Pragasam V, Jenita X, Kalaiselvi P, Muthu V, Varalakshmi P. (2004) Oxidative stress in urogenital tuberculosis patients: a predisposing factor for renal stone formation-amelioration by vitamin E supplementation. Clin. Chim. Acta 350, 57-63 https://doi.org/10.1016/j.cccn.2004.07.001
  33. Staal GEJ, Visser J, Veeger C. (1969) Purification and properties of glutathione reductase of human erythrocytes. Biochim. Biophys. Acta 185, 39-48
  34. Sun Y. (1990) Free radicals, antioxidant enzymes, and carcinogenesis. Free Radic. Biol. Med. 8, 583-599 https://doi.org/10.1016/0891-5849(90)90156-D
  35. Tamilselvan S, Hackett RL, Khan SR. (1997) Lipid peroxidation in ethylene glycol induced hyperoxaluria and calcium oxalate nephrolithiasis. J. Urol. 157, 1059-1063 https://doi.org/10.1016/S0022-5347(01)65141-3
  36. Thamilselvam S, Menon M. (2005) Vitamin E therapy prevents hyperoxaluria-induced calcium oxalate crystal deposition in the kidney by improving renal tissue antioxidant status. B. J. U. Int. 96, 117-126 https://doi.org/10.1111/j.1464-410X.2005.05579.x
  37. Thamilselvan S, Khan SR, Menon M. (2003) Oxalate and calcium oxalate mediated free radical toxicity in renal epithelial cells: effect of antioxidants. Urol. Res. 31, 3-9
  38. Tungsanga K, Sriboonlue P, Futrakul P, Yachantha C, Tosukhowong P. (2005) Renal tubular cell damage and oxidative stress in renal stone patients and the effect of potassium citrate treatment. Urol. Res. 33, 65-69 https://doi.org/10.1007/s00240-004-0444-4
  39. Ulluwisheva RK. (1991) Modernisation versus sustainability. Environ. Conserv. 18, 103-109
  40. Vetrichelvan T, Jegadeesan M, Senthil Palaniappan M, Murali NP, Sasikumar K. (2000) Diuretic and anti-inflammatory activities of Aerva lanata in rats. Indian J. Pharm. Sci. 4, 300-305
  41. Vetrichelvan T, Jegadeesan M. (2002) Anti-diabetic activity of alcoholic extract of Aerva lanata (L.) Juss. ex Schultes in rats. J. Ethnopharmacol. 80, 103-107 https://doi.org/10.1016/S0378-8741(01)00412-3
  42. Vijayavel K, Anbuselvam C, Balasubramanian MP. (2006) Free radical scavenging activity of the marine mangrove Rhizophora apiculata bark extract with reference to naphthalene induced mitochondrial dysfunction. Chem. Biol. Interact. 163, 170-175 https://doi.org/10.1016/j.cbi.2006.06.003
  43. Zapesochnaya GG, Okhanov V, Kurkin V, Kiroshnikov A. (1992) Cathine-6-one and beta carboline alkaloids from Aerva lanata. Planta Med. 37, 192-196 https://doi.org/10.1055/s-2006-961427