Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Immunohistochemical Study on the Effect of Dexamethasone on the Luteolysis of Corpus Luteum of the Rat

Dexamethasone이 황체용해에 미치는 영향에 관한 면역조직화학적 연구

  • Park, Sun-Hee (Department of Obstetrics and Gynecology, College of Medicine, Chungnam National University) ;
  • Ko, Young-Bok (Department of Obstetrics and Gynecology, College of Medicine, Chungnam National University) ;
  • Rhee, Yun-Ee (Department of Obstetrics and Gynecology, College of Medicine, Chungnam National University) ;
  • Noh, Heung-Tae (Department of Obstetrics and Gynecology, College of Medicine, Chungnam National University) ;
  • Kim, Won-Sik (Depatment of Anatomy, College of Medicine, Chungnam National University)
  • 박선희 (충남대학교 의과대학 산부인과학교실) ;
  • 고영복 (충남대학교 의과대학 산부인과학교실) ;
  • 이윤이 (충남대학교 의과대학 산부인과학교실) ;
  • 노흥태 (충남대학교 의과대학 산부인과학교실) ;
  • 김원식 (충남대학교 의과대학 해부학교실)
  • Published : 2008.03.30
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Abstract

Objective: This study was attempted to look at the effect of dexamethasone on the luteolysis of corpus luteum in rats by immunohistochemical study. Methods: Counting with an optical microscope was conducted to make a comparison on difference in luteolysis and penetration of macrophage into three groups: control group of 30 female rats at 8 weeks of age, dexamethasone 0.1 mg administered group, and dexamethasone 1mg administered group. Results: As a result of TUNEL immunostaining, the percentage of luteolysis was significantly reduced in both dexamethasone 0.1 mg administered group and 1 mg administered group, and after ED1 immunostaining, macrophage invasion was reduced in dexamethasone 1 mg administered group. As a consequence of ED1 immunostaining, the immune response of macrophage was much decreased in dexamethasone 1 mg administered group than control group. Conclusion: Dexamethasone works on luteal cell, so it can suppress apoptosis. It can suppress luteolysis by suppression macrophage invasion into corpus luteum or suppress macrophage activation in corpus luteum.

목 적: Glucocorticoid가 황체용해에 미치는 효과의 기전의 일부를 면역 조직학적 연구를 통해 알아보고자 하였다. 연구방법: 8주령의 Rat 암컷 30마리 대조군, 덱사메타손 0.1 mg 투여군 및 덱사메타손 1mg 투여군의 3군으로 나누어 황체용해의 차이와 대식세포의 침투여부를 비교하기 위하여 광학현미경을 이용하여 계수하였다. 결 과: TUNEL 면역염색 결과 덱사메타손 0.1 mg 투여군과 1 mg 투여군 모두에서 대조군에 비해 황체용해의 비율이 현저히 감소하였고, ED1 면역염색 결과 덱사메타손 1 mg 투여군에서 대식세포의 황체 내 침투가 현저히 억제된 것으로 나타났다. ED1 면역염색 결과 덱사메타손 1 mg 투여군에서 대식세포의 면역반응성이 대조군에 비해 감소되어 나타났다. 결 론: 덱사메타손은 직접 황체세포에 작용하여 세포자멸사를 억제한다. 또한 대량 투여했을 때는 대식세포의 황체 내 침투를 억제하거나 황체 내에 이미 존재하는 대식세포의 활성화를 억제함으로써 황체용해를 억제한다.

Keywords

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