Journal of Microbiology and Biotechnology

  • 제18권11호
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  • Pages.1785-1788
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  • 2008
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Inhibition of Diacylglycerol Acyltransferase by Phenylpyropenes Produced by Penicillium griseofulvum F1959

  • Lee, Seung-Woong (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Rho, Mun-Chual (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Choi, Jung-Ho (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Koan-Hoi (Department of Pharmacology, Pusan National University-School of Medicine) ;
  • Choi, Yong-Seok (School of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Hyun-Sun (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Young-Kook (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 2008.11.30
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초록

Phenylpyropenes A, B, and C, isolated from Penicillium griseofulvum F1959, inhibited DGAT in rat liver microsomes with $IC_{50}$ values of $78.7{\pm}1.6$, $21.7{\pm}0.2$, and $11.04{\pm}0.2{\mu}M$, respectively. In addition, a kinetic analysis using a Lineweaver-Burk plot revealed that phenylpyropene C was a noncompetitive inhibitor of DGAT. The apparent Michaelis constant ($K_m$) value and inhibition constant ($K_i$) value were calculated to be $8{\mu}M$ and $10.48{\mu}M$, respectively. Moreover, phenylpyropene C inhibited triglyceride formation in HepG2 cells.

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참고문헌

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피인용 문헌

  1. Triacylglycerol synthesis and energy metabolism: a gut reaction? vol.4, pp.6, 2008, https://doi.org/10.2217/clp.09.67
  2. Therapeutic Strategies for Metabolic Diseases: Small-Molecule Diacylglycerol Acyltransferase (DGAT) Inhibitors vol.9, pp.11, 2008, https://doi.org/10.1002/cmdc.201402069