Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Physicochemical Properties of an Anti-Yeast Substance Produced by Rahnella aquatilis Strain AY2000

Rahnella aquatilis AY2000균주가 생산하는 항효모성 물질의 이화학적 특성

  • Kang, Min-Jung (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Lee, Bok-Kyu (Department of Molecular Biology, Dong-Eui University) ;
  • Lee, Eun-Woo (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Kim, Kwang-Hyeon (Department of Life Science and Biotechnology, Dong-Eui University)
  • 강민정 (동의대학교 자연과학대학 생명응용학과) ;
  • 이복규 (동의대학교 자연과학대학 분자생물학과) ;
  • 이은우 (동의대학교 자연과학대학 생명응용학과) ;
  • 김광현 (동의대학교 자연과학대학 생명응용학과)
  • Published : 2008.12.28
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Abstract

Rahnella aquatilis strain AY2000 produces an anti-yeast substance (AYS), however activity of the AYS has a declining tendency during storage. To investigate what has been decreased activity of the AYS, the AYS was treated with various physicochemical agents in this paper. The activity of AYS was decreased by heat treatment. Thiol reagent such as $\beta$-mercaptoethanol or dithiothreitol was also another factor decreasing the activity of AYS. However, pH, EDTA, and NaCl were not factors decreasing the activity of AYS. Use of methanol to precipitate the AYS was also decreased the activity of AYS. The activity of AYS was not lost after Sepharose S-400 gel filtration. However, the AYS activity was completely lost, when a polysaccharide and a unknown substance (230 nm absorption) among components of the AYS was separated by DEAE-cellulose chromatography. MIC of the AYS against S. cerevisiae was usually determined at $7.8-15.6{\mu}g/ml$.

Rahnella aquatilis AY2000 균주는 항효모성 물질(AYS)을 생산하지만, AYS가 저장 중에 활성이 감소되는 경향이 있었다. 따라서 본 연구에서는 AYS활성이 저하되는 원인을 알아보기 위하여 AYS에 다양한 이화학적인 처리를 행하였다. 그 결과 열처리가 AYS의 활성을 감소시키는 하나의 인자이며, AYS를 침전시키는 유기용매인 methanol의 사용 또한 AYS의 활성을 감소시키는 원인이었다. 또한 $\beta$-mercaptoethanol 과 dithiothreitol 등 thiol화합물 역시 AYS의 활성을 감소시키는 인자로 작용하였으나, pH, EDTA나 NaCl은 AYS의 활성저하를 가져오지 않았다. 한편, 정제과정에서 AYS의 조성분 중 다당류와 미지의 물질(230 nm)을 DEAE-cellulose 이온교환수지로 분리시키면 AYS의 활성이 완전히 소실되지만, AYS를 Sephacryl S-400 gel 여과를 행하여 이들 성분이 분리되지 않은 상태에서는 그 활성이 잘 유지되었다. 본 실험에 사용된 AYS의 MIC는 S. cerevisiae 대해 $7.8-15.6{\mu}g/mL$ 범위로 측정된다.

Keywords

References

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