Journal of Oral Medicine and Pain

Korean Academy of Orofacial Pain and Oral Medicine (대한안면통증구강내과학회)
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Effect of Glucose at High Concentrations on the Integrin and Cathepsin Expression of the Cultured Human Periodontal Ligament Cells

고농도 포도당이 사람 치주인대세포의 Integrin과 Cathepsin 발현에 미치는 영향에 관한 연구

  • Kim, Bang-Soo (Department of Oral Anatomy, School of Dentistry, Kyung Hee University) ;
  • Shin, Je-Won (Department of Oral Anatomy, School of Dentistry, Kyung Hee University) ;
  • Hong, Jung-Pyo (Department of Oral Medicine School of Dentistry, Kyung Hee University) ;
  • Jue, Seong-Suk (Department of Oral Anatomy, School of Dentistry, Kyung Hee University)
  • 김방수 (경희대학교 치의학전문대학원 구강해부학교실) ;
  • 신제원 (경희대학교 치의학전문대학원 구강해부학교실) ;
  • 홍정표 (경희대학교 치의학전문대학원 구강내과학교실) ;
  • 주성숙 (경희대학교 치의학전문대학원 구강해부학교실)
  • Published : 2008.03.30
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Abstract

This experiment was designed to clarify the effect of high concentrations of extracellular glucose on the periodontal ligament cells. The cells were incubated for 24 and 48 hours with ${\alpha}-MEM$ including 1,000 mg/L(control group) and 4,500 mg/L(experimental group) of glucose. Then, the expressions of ${\alpha}5$ integrin, cathepsin-B and -L were examined using RT-PCR method. The results were as follows: 1. ${\alpha}5$ integrin expression was increased after incubation in high glucose media. 2. Cathepsin-B expression was increased after the 24-H incubation with high glucose media, but was decreased after the 48-H incubation. 3. The expression of cathepsin-L was decreased by the high glucose media. Theses results suggest that extracellular glucose at high concentration may be attributed to delayed wound healing in diabetes patients through increase in ${\alpha}5$ integrin and decrease in cathepsins, which lead to accumulation of extracellular matrices and adhesion molecules.

고농도 세포외 포도당이 치주인대세포의 integrin 발현과 cathepsin-B 및 -L의 발현에 미치는 영향을 살펴보기 위하여 사람 치주인대로부터 일차배양을 통해 얻은 치주인대세포를 1,000 mg/L 농도의 포도당이 포함된 배양액(대조군)과 4,500mg/L 농도의 포도당이 포함된 배양액(실험군)으로 나누어 24시간과 48시간 배양하였다. 그 후, RT-PCR을 통하여 integrin, cathepsin-B 및 -L의 발현을 평가하여 다음과 같은 결론을 얻었다. 1. 대조군에 비해 실험군에서 ${\alpha}5$ intergrin 발현이 증가하였다. 2. Cathepsin-B는 24시간 배양 실험군에서 발현이 증가하였으나, 48시간 배양후에는 발현이 감소하였다. 3. Cathepsin-L은 24시간과 48시간 배양군 모두에서 대조군에 비해 실험군에서 발현이 감소하였다. 이상의 결과로 보아, 고농도 포도당 조건은 치주인대세포의 integrin 발현을 증가시키며, 이는 세포활성에 영향을 미쳐 치주조직의 재생을 지연시킬 것으로 추측된다. 또한, 이 과정은 cathepsin 발현의 감소로 인해 촉진될 것으로 생각된다.

Keywords

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