Baicalin이 조골세포의 생성 및 활성에 미치는 영향

Effects of Baicalin on the Proliferation and Activity of Osteoblastic Cells

  • 고선일 (단국대학교 치과대학 구강생화학교실)
  • Ko, Seon-Yle (Department of Oral Biochemistry, School of Dentistry, Dankook University)
  • 발행 : 2008.06.30

초록

Baicalin은 Scutellaria baicalensis에서 분리되는 flavonoid의 일종으로, 다양한 생물학적 활성을 나타내는 물질로 알려져 있다. Baicalin은 항균, 항염증, 진통작용을 나타내며, nuclear factor-kappaB의 활성을 억제한다고 보고되었다. 최근에 다양한 flavonoid 들이 골조직 대사에 관여함이 밝혀졌으며, 본 연구에서는 baicalin이 골조직의 주요세포인 조골세포의 생성 및 활성에 미치는 영향을 관찰하기 위하여, 세포증식율, 세포생존율, 염기성 인산분해효소 활성 및 osteoprotegerin 생성량의 변화를 관찰하였다. 그 결과 baicalin은 조골세포의 세포 증식과 생존율에는 영향을 미치지 못하였으나, 염기성 인산분해효소의 활성과 osteoprotegerin의 생성량을 증가시켰다. 따라서 baicalin은 골조직에서 조절물질로 역할을 할 것으로 예견된다.

Baicalin is a flavonoid compound isolated from the medicinal plant Scutellaria baicalensis. It is known to affect multiple biological functions, including of antibacterial, anti-viral, anti-inflammatory and analgesic effects. Baicalin can inhibit nuclear factor-kappaB activation. It has been reported that some flavonoids possess the effects of bone metabolism. The present study was undertaken to determine the possible cellular mechanism of action of baicalin in osteoblasts. The effects on the osteoblast were determined by measuring cell proliferation, cell viability, alkaline phosphatase activity, and osteoprotegerin secretion. Baicalin has no effect on the osteoblastic cell proliferation and cell viability. Baicalin treatment showed increase in alkaline phosphatase activity and osteoprotegerin secretion of osteoblasts. Thus, baicalin may be a regulatory protein within the bone.

키워드

참고문헌

  1. Wasnich R. What is an osteoporotic fracture? in Osteoporosis: Diagnostic and Therapeutic Principles, In Rosen CJ(Ed), Humana Press, Totowa, 1996, NJ, pp. 79-88
  2. Rodan GA. Emerging therapies in osteoporosis. Ann Rep Med Chem 1994;29:275-285 https://doi.org/10.1016/S0065-7743(08)60741-1
  3. Reginster JY. Treatment of bone in elderly subjects: calcium, vitamin D, fluor, bisphosphonates, calcitonin. Horm Res 1995;43:83-88 https://doi.org/10.1159/000184243
  4. Stock JL. Drug therapy. in Osteoporosis: Diagnotic and Therapeutic Principles, In Rosen CJ(Ed), Humana Press, Totowa, 1996, NJ, pp. 173-187
  5. Yeung HC. Handbook of Chinese Herbs and Formulas, Institute of Chinese Medicine, Los Angeles, 1985
  6. Ha H, Kwak HB, Lee SW et al. Reactive oxygen species mediate RANK signaling in osteoclasts. Exp Cell Res 2004;301:119-127 https://doi.org/10.1016/j.yexcr.2004.07.035
  7. Reddy SV. Regulatory mechanisms operative in osteoclasts. Crit Rev Eukaryot Gene Expr 2004;14: 255-270 https://doi.org/10.1615/CritRevEukaryotGeneExpr.v14.i4.20
  8. Gao Z, Huang K, Yang X et al. Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. Biochim Biophys Acta 1999;16:643-650
  9. Zhang DW, Cheng Y, Wang NL et al. Effects of total flavonoids and flavonol glycosides from Epimediurn koreanum Nakai on the proliferation and differentiation of primary osteoblasts. Phytomedicine 2008;15:55-61 https://doi.org/10.1016/j.phymed.2007.04.002
  10. Zhang Y, Zeng X, Zhang L et al. Stimulatory effects of puerarin on bone formation through activation of PI3K/Akt pathway in rat calvaria osteoblasts. Planta Med 2007;73:341-347 https://doi.org/10.1055/s-2007-967168
  11. Choi EM. Apigenin increases osteoblastic differentiation and inhibits tumor necrosis factor-alphainduced production of interleukin-6 and nitric oxide in osteoblastic MC3T3-E1 cells. Pharmazie 2007;62: 216-220
  12. Li Y, Ji H, Li P et al. Effects of Epimedium pubescens flavonoids on osteoblast inclined form. J Chinese Pharm Univ 2002;33:48-50
  13. Wang GF, Wu ZF, Wan L et al. Influence of baicalin on the expression of receptor activator of nuclear factor-kappaB ligand in cultured human periodontal ligament cells. Pharmacology 2006;77:71-77 https://doi.org/10.1159/000092853
  14. Owen ME. Lineage of osteogenic cells and their relationship to the stromal system. in Bone and Mineral Research. In Peck WA (Ed). Amsterdam, 1985, Elsevier Science Publisher, pp. 1-25
  15. Martin JH, Matthews JL. Mitochondrial granules in chondrocytes, osteoblasts and osteocytes. An ultrastructural and microincineration study. Clin Orthop 1970;68:273-278
  16. Hidaka S, Okamoto Y, Nakajima K. Preventive effects of traditional Chinese medicines on experimental osteoporosis induced by ovariectomy in rats. Caicif Tissue Int 1997;61:239-246 https://doi.org/10.1007/s002239900329
  17. Chen KM, Ge BF, Ma HP et al. The serum of rats administered flavonoid extract from Epirnedium sagittatum but not the extract itself enhances the development of rat calvarial osteoblast-like cells in vitro. Pharmazie 2004;59:61-64
  18. Xie F, Wu CF, Lai WP et al. The osteoprotective effect of Herba epimedii (HEP) extract in vivo and in vitro. eCAM 2005;2:353-361 https://doi.org/10.1093/ecam/neh101
  19. Wang JQ, Hu YG, Zheng HJ et al. The effect of icarun on proliferation and differentiation of osteoblasts in vitro. Chinese J Clin Rehabil 2002;6: 1307-1308
  20. Han LM, Liu B, Xu P. Influence of Herba epimedii flavones on proliferation of osteoblast. Shanghai J tradit Chinese Med 2003;37:56-58
  21. Meng FH, Li YB, Xiong ZL et al. Osteoblastic proliferative activity of Epimedium breoicornum Maxim. Phytomedicine 2005;12:189-193 https://doi.org/10.1016/j.phymed.2004.03.007
  22. Liu HC, Chen RM, Jian WC et al. Cytotoxic and antioxidant effects of the water extract of the traditional Chinese herb gusuibu tDrunaria fortuneii on rat osteoblasts. J Formos Med Assoc 2001;100: 383-388
  23. Notoya M, Tsukamoto Y, Nishimura H et al. Quercetin, a flavonoid, inhibits the proliferation. differentiation, and mineralization of osteoblasts in vitro. Eur J Pharmacol 2004;485:89-96 https://doi.org/10.1016/j.ejphar.2003.11.058
  24. Shen YC, Chiou WF, Chou YC et al. Mechanisms in mediating the anti -inflammatory effects of baicalin and baicalein in human leukocytes. Eur J Pharmacol 2003;465: 171-181 https://doi.org/10.1016/S0014-2999(03)01378-5
  25. Song YZ, Yang YZ, Jia YZ. Antagonistic effect of baicalin on oxidative stress iNJury in neurons and astrocytes of rats. Zhongguo Zhong Xi Yi Jie He Za Zhi 2004;24: 339-342
  26. Liu WC, Li XW, Li YS et al. Effects of baicalinmodified poly (D,L-lactic acid) surface on the behavior of osteoblasts. J Mater Sci Mater Med 2003;14:961-965 https://doi.org/10.1023/A:1026398532009
  27. Siffert RS. The role of alkaline phosphatase in osteogenesis. J Exp Med 1951;93:415-422 https://doi.org/10.1084/jem.93.5.415
  28. Fauran-Clavel MJ, Oustrin J. Alkaline phosphatase and bone calcium parameters. Bone 1986;7:95-99 https://doi.org/10.1016/8756-3282(86)90680-0
  29. Kostenuik PJ, Shalhoub V. Osteoprotegerin: A physiological and pharmacological inhibitor of bone resorption. Curr Pharm Des 2001;7:613-635 https://doi.org/10.2174/1381612013397807