Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Decreased of Clusterin mRNA Expression of Epididymis Following Exposure to Bisphenol A Diglycidyl Ether during Gestation and Lactation in Sprague-Dawley Rats

임신 및 수유기간 동안 Bisphenol A Diglycidyl Ether 노출에 의한 랏트 부고환 clusterin mRNA 발현량 감소

  • Kang, Dae-Woong (Department of Preventive Medicine, College of Medicine, Chung-Ang University) ;
  • Kwon, Su-Kyoung (Department of Preventive Medicine, College of Medicine, Chung-Ang University) ;
  • Yang, Yun-Jung (Department of Preventive Medicine, College of Medicine, Chung-Ang University) ;
  • Chun, Young-Jin (College of Pharmacy, Chung-Ang University) ;
  • Hong, Yeon-Pyo (Department of Preventive Medicine, College of Medicine, Chung-Ang University)
  • 강대웅 (중앙대학교 의과대학 예방의학교실) ;
  • 권수경 (중앙대학교 의과대학 예방의학교실) ;
  • 양윤정 (중앙대학교 의과대학 예방의학교실) ;
  • 천영진 (중앙대학교 약학대학) ;
  • 홍연표 (중앙대학교 의과대학 예방의학교실)
  • Published : 2008.12.30
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Abstract

Bisphenol A diglycidyl ether(BADGE)는 비스페놀 A와 에피클로로하이드린의 축합에 의해 만들어지는 물질로 상업용 액상 에폭시 수지의 주성분이다. 본 연구는 clusterin mRNA 발현이 BADGE의 노출된 생식기계 독성에 연관되어 있는지를 연구하기 위해 수행하였다. BADGE는 SPF Sprague-Dawley 임신 랏트에 임신 6일부터 수유기가지 하루에 한 번 0(대조군)과 375mg/kg/day를 경구 투여하였다. 수컷 새끼는 일반 사항과 몸무게, 일반 발달 지표(예, 항문과 생식기 사이의 거리, 이개개전, 절치붕출, 유두잔류, 안검개열, 고환하강, 포피박리 등)등을 관찰하였다. 대조군과 투여군에서 다섯 마리의 수컷 새끼는 출생 후 3, 6와 9주에 부검하여 부고환의 조직학적 변화 등을 관찰하였다. BADGE 375 mg/kg/day 투여군에서 항문과 생식기 사이의 거리는 대조군보다 길어지는 경향을 보였다. 출생 후 6주와 9주에서 부고환의 상대 무게는 대조군보다 약간 증가하였으나 조직학적인 변화는 관찰되지 않았다. BADGE 투여 군에서 clusterin mRNA 발현량은 대조군에 비해 3주에 56%, 6주에 57% 그리고 9주에 86% 감소하였다. 이런 결과는 랏트의 부고환에서 clusterin은 BADGE에 반응하는 유전자 중 하나일 수 있다는 가능성을 나타낸다.

Keywords

References

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