KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Comparison of Mycophenolic Acid Production by P. brevicompactum Mutants Induced through UV and NTG Treatments

자외선 및 NTG 돌연변이 유도에 의한 Penicillium brevicompactum 변이주의 Mycophenolic Acid (MPA) 생산성 비교

  • Um, Byung-Hwan (Department of Chemical and Biological Engineering, University of Maine) ;
  • Choi, Ju-Young (Department of Applied Chemical Engineering, Dankook University) ;
  • Ha, Byung-Jhip (R&D Center, Cotde Co., Ltd) ;
  • Kim, Young-Soo (Department of Applied Chemical Engineering, Dankook University) ;
  • Oh, Kyeong-Keun (Department of Applied Chemical Engineering, Dankook University)
  • 엄병환 (메인주립대 공과대학 화학생물공학과) ;
  • 최주영 (단국대학교 공학대학 응용화학공학과) ;
  • 하병집 ((주)콧데 기업부설연구소) ;
  • 김영수 (단국대학교 공학대학 응용화학공학과) ;
  • 오경근 (단국대학교 공학대학 응용화학공학과)
  • Published : 2008.10.31
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Abstract

Recently, importance of immunosuppression is increasing as internal organ transplant becomes more prevalent with development of medical technology. Mycophenolic acid (MPA) is a selective inhibitor of guanine synthesis and it therefore has antibacterial, antiviral, antitumor and selective immunosuppressive activities. The objective of this study was to maximize MPA productivity through utilizing the MPA generating strain of Penicillium brevicompactum ATCC 16024, by inducing UV mutation and NTG mutation. The highest MPA obtained was 1.146 g/L, 2.051 g/L, and 1.390 g/L from P. brevicompactum UB-3, UB-9, UC-4 respectively mutants derived from UV treatment. P. brevicompactum NC-3 and NA-9 induced from NTG treatment yielded. 575 g/L, 2.238 g/L of MPA production respectively. Mutants capable of the highest observed production of MPA were P. brevicompactum UB-9 and P. brevicompactum NC-3 obtained using the UV and NTG treatments respectively.

Keywords

References

  1. Jekkel, A. et al. (2001), Microbiological transformation of mycophenolic acid, J of Molecular Catalysis B: Enzymatic 11, 423-426 https://doi.org/10.1016/S1381-1177(00)00146-6
  2. Allison, A. C. and Eugui, E. M. (2000), Mycophenolate mofetil and its mechanisms of action, Immunopharmacology 47, 85-118 https://doi.org/10.1016/S0162-3109(00)00188-0
  3. Sollinger, H. W. (1995), Mycophenolate Mofetil for the Prevention of Acute Rejection in Primary Cadaveric Renal Allograft Recipients, Transplantation 60, 225-232 https://doi.org/10.1097/00007890-199508000-00003
  4. Hattori, K. and T. Suzuki (1974), Large scale production of erythritol and its conversion to D-mannitol production by n-alkane grown, Agri. Biol. Chem. 38, 1203-1208 https://doi.org/10.1271/bbb1961.38.1203
  5. Aoki, M. A. Y. et al. (2000), Microbial transformation of sucrose and glucose to erythritol, Biotechnology Letters 15, 383-362
  6. Noh, B. S. et al. (2000), Optimization of Culture Conditions for Erythritol Production by Torula sp., J. of Microbiology and Biotechnology 10, 69-74
  7. Choi, D. B., K. A. Cho, W. S. Cha, and S. R. Ryu (2004), Effect of Triton X-100 on Compactin Production from Penicillium citrinum, Biotechnol. Bioprocess Eng. 9, 171-178 https://doi.org/10.1007/BF02942288
  8. Park, M. H., H. J. Ryu, and K. K. Oh (2004), Isolation of Lipase Producing Yeast and Optimization of Cultivation Condition, Korean J. Biotechnol. Bioeng. 19(2), 148-153
  9. Yoon, S. H., M. S. Ko, K. A. Park, K. H. Jung,Y. C. Shin, Y. M. Lee, S. H. Lee, and S. W. Kim (2006), Enhabnced Lycopene Production in Recombinant Escherichia coli by Random Transposon and NTG Mutagenesis, Korean J. Biotechnol. Bioeng. 21(2), 90-95