The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Purification and Phytotoxicity of Apicidins Produced by the Fusarium semitectum KCTC16676

  • Jin, Jianming (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Baek, Seung-Ryel (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Lee, Kyung-Rim (Department of Biology, University of Incheon) ;
  • Lee, Jungkwan (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University) ;
  • Yun, Sung-Hwan (Department of Medical Biotechnology, Soonchunghyang University) ;
  • Kang, Seog-Chan (Department of Plant Pathology, The Pennsylvania State University) ;
  • Lee, Yin-Won (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University)
  • Published : 2008.12.01
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Abstract

Apicidin is a cyclic tetrapeptide produced by some Fusarium species and is known to inhibit Apicomplexan histone deacetylase. The goals of this study were to determine species identity of Fusarium isolate KCTC16676, an apicidin producer, to improve a method for apicidin extraction, and to test phytotoxicity of apicidin and its analogs. We compared sequences of the translation elongation factor 1-alpha (TEF) gene in KCTC16676 with those from isolates representing diverse Fusarium species, which showed that KCTC16676 belongs to the F. semitectum-F. equiseti species complex. To enhance apicidin production, after culturing isolate KCTC16676 on a wheat medium for 3 weeks at $25^{\circ}C$, the culture was extracted with chloroform. Apicidins were purified through a reverse phase $C_{18}$ silica gel column, resulting in 5 g of apicidin, 200 mg of apicidin A, and 300 mg of apicidin $D_2$ from 4 kg of wheat cultures; this represents a significant yield improvement from a previous method, offers more materials to study the modes of its action, and facilitates the elucidation of the apicidin biosynthesis pathway. Apicidin and apicidin $D_2$ showed phytotoxicity on both seedlings and 2-week-old plants of diverse species, and weeds were more sensitive to apicidins than vegetables

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References

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