Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Enhancement of Immune-Potentiation of Cichorium endivia L. by Ultrasonification Extraction Process

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  • Kwon, Min-Chul (College of Bioscience & Biotechnology, Kangwon National Univ.) ;
  • Han, Jae-Gun (College of Bioscience & Biotechnology, Kangwon National Univ.) ;
  • Qadir, Syed Abdul (College of Bioscience & Biotechnology, Kangwon National Univ.) ;
  • Ahn, Ju-Hee (College of Bioscience & Biotechnology, Kangwon National Univ.) ;
  • Lee, Dal-Ho (Sorak traditional foods Co.) ;
  • Lee, Hyeon-Yong (Research Institute of Bioscience and Biotechnology, Kangwon National University)
  • 권민철 (강원대학교 BT특성화학부(대학)) ;
  • 한재건 (강원대학교 BT특성화학부(대학)) ;
  • ;
  • 안주희 (강원대학교 BT특성화학부(대학)) ;
  • 이달호 (설악전통식품) ;
  • 이현용 (강원대학교 생명공학연구소)
  • Published : 2008.02.29
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Abstract

Immune-potentiation of Chicorium endivia L. were investigated on follows extracts associated with ultrasonification process at 60 kHz and showed the highest promotion of human B and T cell growth, about $10{\sim}20%$ compared to the control. The secretion of TNF-${\alpha}$ and IL-6 was also enhanced by the addition $(0.5mg/m{\ell})$ of the extracts. NK cell activation was Improved up to 1.37 times higher than the control, through adding extracts. It was also found that extracts from C. endivia L. could yield higher nitric oxide production from macrophage than Lipopolysaccaharides (LPS). It can be concluded that, in general, the extracts treated with ultrasonification has higher immune activity than others, possibly by higher yielding immune-modulatory activity than conventional extraction process. The optimum condition for the extraction of C. endivia L. is ethanol extraction at $60{\sim}100^{\circ}C$ associated with ultrasonification.

Keywords

References

  1. Bae JH, Park KW, Kang HM (2005) Effects of packing materials, light condition and storage temperature on MAP storage of chicon. Journal of Bio-Environment control 14(2):69-75
  2. Cecilia K, Limdbolum (2002) IL-2 receptor signaling through the Shb adapter protein in T and NK cells. Biochemical and Biophysical Research Communications 296:929-936
  3. Chung K, Kim WI, Hong IK, Park KA (2000) Ultrasonic energy effects on squalene extraction from amaranth seed. Applied Chemistry 4(2):149-152
  4. Ding AH, Nathan CF, Stuhr DJ (1998) Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. J. Immunol. 141:2407-2412
  5. Han BH, Park MH, Cho JY, Park JS, Yoo ES, Baik KU (1998) Effect of ginsenosides from Panax ginseng on $TNF-{\alpha}$ production and T cell proliferation. Yakhak Hoeji 42(3):296-301
  6. Kang HI, Kim JY, Moon KD, Seo KI, Cho YS, Lee SD, Yee ST (2004) Effect of the crude polysaccharide of Pleurotus eryngii on the activation of immune cells. J. Kor. Soc. Food Sci. Nutr. 33:1092-1097 https://doi.org/10.3746/jkfn.2004.33.7.1092
  7. Kim DH, Park JH, Kim JH, Kim CH, You JH, Kwon MC, Lee HY (2005) Enhancement of immune activities of Ephedrae Herba and Rubi Fructus at low temperature extraction. Korean J. Medicinal Crop Sci. 13(3):81-86
  8. Kim JH, Kim DH, You JH, Kwon MC, Lee HJ, Lee HJ, Lee HY (2005) Anticancer and immune activities of the extracts from Amorpha fruticosa L. Korean J. Medicinal Crop Sci. 13(1):41-47
  9. Kim SW, Kim ES (1997) Studies on the immunomodulating effect of polysaccharide extracted from Ganoderma lucidum on macrophage. J. Korean Soc. Food Sci. Nutr. 26:148-153
  10. Kim WI, Shung KW, Lee SB, Hong IK, Park KA (2001) Ultrasound energy effects on solvent extraction of amaranth seed oil. J. Korean Ind. Eng. Chem. 12(3):307-311
  11. Kwon OW, Kim CH, Kim HS, Kwon MC, Ahn JH, Lee HJ, Kang HY, Lee HY (2007) Comparison of immuno modulatary and anticancer activities according to the parts of the Styrax japonica Sieb. et Zucc. Korean J. Medicinal Crop Sci. 15(3):170-176
  12. Lee MK, Choi GP, Ryu LH, Lee GY, Yu CY, Lee HY (2004) Enhanced immune activity and cytotoxicity of Artemisia capillaris Thunb. extracts against human cell lines. Korean J. Medicinal Crop Sci. 12(1):36-42
  13. Lee MK, Lee SH, Choi GP, Yu CY, Lee JH, Lee HY (2003) Screening of immune enhancement activities of the extracts from Rosa rugosae Radix. Korean J. Medicinal Crop Sci. 11(1):13-18
  14. Lee SH, Lee HS, Park YS, Hwang B, Kim JH, Lee HY (2002) Screening of immune activation activities in the leaves of Dendropanax morbifera Lev. Korean J. Medicinal Crop Sci. 10(2):109-115
  15. Park JH, Lee HS, Mun HC, Kim DH, Seong NS, Jung HG, Bang JK, Lee HY (2004) Improvement of anticancer activation of ultrasonificated extracts from Acanthopanax senticosus Harms, Ephedra sinica stapf, Rubus coreanus miq. and Artemisia capillaris Thunb. Korean J. Medicinal Crop. Sci. 12(4):273-278
  16. Shim MS, Choi SW, Bae SJ (2001) Effects of Punica granatum L. fractions on quinone reductase induction and growth inhibition on several cancer cells. J. Korean Soc. Food Sci. Nutr. 30:80-85
  17. Yueran Z, Rui S, You L, Chunyi G, Zhigang T (2003) Expression of leptin recrptors and response to leptin stimulation of human natural killer cell lines. Biochemical and Biophysical Research Communications 300:247-252 https://doi.org/10.1016/S0006-291X(02)02838-3