Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Punica granatum L. Extracts on Serum Lipids Level in Ovariectomized Rats

석류가 갱년기 장애를 유도한 흰쥐의 혈 중 지질 함량에 미치는 영향

  • Published : 2008.01.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study investigated the effects of Punica granatum L. (PG) ethanol extracts on serum lipid levels in ovariectomized rats. In order to elucidate the effects of PG ethanol extracts on the change of lipid concentration in rats, Sprague-Dawley female rats were randomly assigned to groups as follows: sham-operated rats (sham), ovariectomized control rats (OVX-control), ovariectomized rats supplemented with ethanol extracts of PG seed at 50 mg/kg bw/day (OVX-PS50), ovariectomized rats supplemented with ethanol extracts of PG seed at 200 mg/kg bw/day (OVX-PS200), ovariectomized rats supplemented with ethanol extracts of PG peel at 50 mg/kg bw/day (OVX-PS50), ovariectomized rats supplemented with ethanol extracts of PG peel at 200 mg/kg bw/day (OVX-PP200). PG ethanol extracts were orally supplemented at the level of 1mL per day for 7 weeks. The body weight gain, food intake and food efficiency ratio were not significantly different between groups by t-test. The levels of total-cholesterol and triglyceride in serum were not changed by supplementation of PG ethanol extracts. The level of HDL-cholesterol on serum of the OVX-PS50 and OVX-PS200 groups were similar to the OVX-control group, but the OVX-PP50 and OVX-PP200 groups were higher than sham group. These results suggest that ethanol extracts of PG peel were more effected than those of PG seed.

석류(Punica granatum L.)는 생리활성이 강한 천연성분인 폴리페놀과 탄닌이 많이 함유되어 있어 예로부터 전통요법의 치료제로 사용되어 왔으며, 최근에는 석류의 phytoestrogen을 이용하여 갱년기 치료에 대한 연구가 활발히 진행중이다. 따라서 본 연구에서는 석류의 씨와 껍질의 ethanol 추출물과 석류 껍질 분획물을 이용하여 인위적으로 난소를 절제하여 폐경을 야기 시킨 흰쥐에서의 혈 중 지질 농도의 변화를 검토하였다. 그 결과 혈 중 total-cholesterol 및 triglyceride 농도에 있어서는 석류 ethanol추출물 투여에 의한 유의적인 효과는 없었으나, triglyceride의 경우 석류 껍질 추출물의 $200mg/kg{\cdot}bw/day$투여군에서는 OVX-control에 비하여 낮게 나타났다. 또한 혈 중 HDL-cholesterol농도는 석류씨와 껍질부분을 추출한 모든 실험군에서 동맥경화의 지표로서 관상성 심장질환에 대한 방어 작용을 가지고 있는 HDL-cholesterol 함량이 높게 나타났다. 특히 석류 껍질부분의 ethanol추출물의 경우 OVX-control에 비하여 유의적으로 나타나 갱년기장애로 인한 심혈관계 질환을 예방하는 천연 에스트로겐 대체 식품으로서의 귀추가 주목된다.

Keywords

References

  1. Abe, T., J. W. Chow, M. Lean and T. J. Chambers. 1993. Estrogen does not restore bone lost after ovariectomy in the rat. J. Bone Miner. Res. 8, 831-838. https://doi.org/10.1002/jbmr.5650080709
  2. Ahn, H. S., J. R. kwon and S. S. Lee. 1997. Effect of Dietary Lipids and Guar Gum on Lipid Metabolism in Ovariectimized Rats. The Korean Nutr. Society 30, 1123-1131.
  3. Aiken, J. M., E. Armstrong, and J. B. Anderson. 1972. Osteoporosis after ophoretomy in the mature female rat and the effect of estrogen and/or progesterone replacement therapy in its prevention. J. Endocrinol. 55, 79-87. https://doi.org/10.1677/joe.0.0550079
  4. Baker, H. J., J. R. Lindsey and S. H. Weisbroth. 1984. The laboratory rats. Academic Press Inc., New York, Vol. II, pp. 123-127.
  5. Baker, M., K. L. Medlock and D. M. Sheehan. 1998. Flavonoids inhibit estrogen binding to rat alpha-fetoprotein. Proc. Soc. Exp. Biol. Med. 217, 317-321. https://doi.org/10.3181/00379727-217-44238
  6. Beeson, P. B., W. McDermott and J. B. Wyngaarden. 1979. Text book of medicine. pp. 77-100, Saunders Co., philadelphia.
  7. Bickoff, E. M., A. L. Livingston, A. P. Hendrickson and A. N. Booth. 1962. Forage estrogens. Relative potencies of several estrogen-like compounds found in forages. J. Agric. Food Chem. 10, 410-415. https://doi.org/10.1021/jf60123a018
  8. Chung, Y. J., S. Y. Lee and K. H. Ahn. 1991. Effects of glycosyl sucrose on blood glucose, bserum lipids and activities of carbohydrate hydrolysing enzymese in rats. J. Korean soc. Food Nutr. 20, 519-526.
  9. Cooper, A. D. 1997. Hepatic uptake of chylomicron remnants. J. Lipid Res. 38, 2173-2192.
  10. Corine, H. R. and S. W. Emma. 1984. Basic nutrition and diet therapy. 5th ed., Macmillan Co., New York, pp. 272-273.
  11. Dietschy, J. M. 1998. Dietary fatty acids and the regulation of plasma low density lipoprotein cholesterol concentrations. J. Nutr. 128, 444S-448S. https://doi.org/10.1093/jn/128.2.444S
  12. Ettinger, B. 1998. Overview of estrogen replacement therapy: a historical perspective. Proc. Soc. Exp. Biol. Med. 217, 2-5. https://doi.org/10.3181/00379727-217-44198
  13. Gil, M. I., F. A, Tomas-Barberan, B. Hess-Pierce, D. M. Holcroft and A. A. Kader. 2000. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J. Agric. Food Chem. 48, 4581-4589. https://doi.org/10.1021/jf000404a
  14. Gordon, T., W. P. Casfelli, M. C. Hiortland, W. B. Kennel and T. R. Dawher. 1977. High density lipoprotein as a protective factor aganist coronary heart diseases, the Framingham study. Am. J. Med. 62, 707-712. https://doi.org/10.1016/0002-9343(77)90874-9
  15. Gorinstein, S., G. W. kulasek, E. Barmikowska, M. Leontowicz, M. Zemser, M. Morawiec and S. Trakhtenberg. 1998. The influence of persimmon peel and persimmon pulp on the lipid metabolism and antioxidant activity of rats fed cholesterol. J. Nutr. Biochem. 9, 223-227. https://doi.org/10.1016/S0955-2863(98)00003-5
  16. Guo, C. J., J. J. Yang, Y. Wei, Y. F. Li, J. Xu and Y. G. Jiang. 2003. Antioxidant activities of peel pulp and seed fractions of common fruits as determined by FRAP assay. Nutr. Research 23, 1719-1726. https://doi.org/10.1016/j.nutres.2003.08.005
  17. Guyton. 1994. Text book of medical physiology. 8th ed., Saunders Co., philadelphia, pp. 754-764.
  18. Kind, P. R. N. and E. J. King. 1954. Estimation of phosphatase by determination of hydrolyzed phenol with aminoantipyrine. J. Clin. Patrol. 7, 322-326. https://doi.org/10.1136/jcp.7.4.322
  19. Kimura, Y., H. Okuda, and K. Mori, 1984. Studies on the activites of tannis and related compouds from medicinal plant and drugs. N. Effects of various extracts of Geranii herba and Geraniin on liver injury and lipid metabolism in rats fed peroxidized oil. Chem. Pharm. Bull. 32, 1866-1871. https://doi.org/10.1248/cpb.32.1866
  20. Kovanen, P. T., M. S. Brown, and J. L. Goldstein. 1979. Increased binding of low density lipoprotein to liver membranes from rats treated with 17 $\alpha$-ethinyl estradiol. J. Biol. Chem. 254, 11367-11373.
  21. Krichevsky, D. 1998. History of recommendations to the public about dietary fat. J. Nutr. 128, 449S-452S. https://doi.org/10.1093/jn/128.2.449S
  22. Kronenberg F, Fugh-Berman A. 2002. Complementary and alternative medicine for menopausal symptoms: a review of randomized, controlled trials. Ann. Int. Med. 137, 805-813. https://doi.org/10.7326/0003-4819-137-10-200211190-00009
  23. Kwak, H. M., H. H. Jeong, B. H. Sohng, J. G. Kim, J. M. Lee, J. M. Hur and K. S. Song. 2005. Quantitative analysis of antioxidants in korean pomegranate peel (Granati pericarpium) cultivated in different site. J. Korean Soc. Appl. Biol. Chem. 48, 431-434.
  24. Li, Y., C. Guo, J. Yang, J. Wei, J. Xu and S. Dheng. 2006. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem. 96, 254-260. https://doi.org/10.1016/j.foodchem.2005.02.033
  25. Miller, N. E., O. H. Forde, D. S. Telle and O. D. Mjos. 1962. The tromoso heart study. High density lipoprotein and coronary heart disease: a prospective case. Lekarstennie srestava Iz rasteniy 8, 378-382.
  26. Nasr, C. B., N. Ayed and M. Metche. 1996. Quantitative determination og the polyphenolic content of pomegranate peel. Zeitschrzfi Fur Lebensmitted Unterschung Und. Forschung 203, 374-478. https://doi.org/10.1007/BF01231077
  27. Olson, R. E. 1998. Discovery of the lipoproteins, their role in fat transport and their significance as risk factors. J. Nutr. 128, 439S-443S. https://doi.org/10.1093/jn/128.2.439S
  28. Park, Y. S., J. Y. Song and T. J. Chung. 1977. Changes of lipids and apoprotein during lipolysis of high density lipoprotein in seurm od liver necrosis patients. Kyemyung Medical Bulletin 4, 28-33.
  29. Rhee, S. J. and G. Y. Park. 1997. Effects of green tea catechin on liver 3-hydroxy-3-methylglutaryl CoA reductase activity and serum lipid levels in streptozotocin - induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 26, 1187-1193.
  30. Rhoades, C. G., C. L. Gulbrandse and A. Kkagan. 1976. Seurm lipoproteins and coronary heart disease in a population study of Hawaii Japanese men, New Emg. J. Med. 294, 293-298.
  31. Reitman, S. and S. Frankel. 1963. A colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am. J. Clin. Pathrol. 28, 56-61.
  32. Seeram N, R. Lee, M. Hardy, D. Heber. 2005. Rapid large scale purification of ellagitannins from pomergranate peel, a by product of the commercial juice industry, Separ. Purif. Technol. 41, 49-55. https://doi.org/10.1016/j.seppur.2004.04.003
  33. Serougne, C; C. Felgines, J. Ferczou, T. Hajri, C. Bertin and A. Mazur, 1995. Hypercholesterolemia induced by cholesterol-or cystine-enriched diets is characterized by different plasma lipoprotein and apolipoprotein concentration in rats. J. Nutr. 125, 34-41.
  34. Wronski, T. J., M. Cintron and L. M. Dann, 1988. Temporal relationship between bone loss and increased bone turnover in ovariectomized rats. Calcif. Tissue Int. 43, 179-183. https://doi.org/10.1007/BF02571317
  35. Yugarani, T., B. K. H. Tan, M. The and N. P. Das. 1992. Effects of polyphenolic natural products on the lipid profiles of rats fed high fat diets. J. Lipids 27, 181-186. https://doi.org/10.1007/BF02536175

Cited by

  1. Quality Characteristics of Yanggaeng containing Pomegranate (Punica granatum) Powder vol.27, pp.5, 2014, https://doi.org/10.9799/ksfan.2014.27.5.906