Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Establishment of an Antibacterial Yeast That Producing Bacteriocin Subpeptin JM4-A or Subpeptin JM4-B

박테리오신 Subpeptin JM4-A 혹은 Subpeptin JM4-B를 생산하는 항균 효모의 제작

  • Lee, Ok-Hee (Department of Bioscience and Biotechnology, Silla University) ;
  • Jang, Min-Kung (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee, Dong-Geun (Department of Bioscience and Biotechnology, Silla University) ;
  • Kim, In-Hae (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, Silla University) ;
  • Ha, Jong-Myung (Department of Bioscience and Biotechnology, Silla University) ;
  • Ha, Bae-Jin (Department of Bioscience and Biotechnology, Silla University) ;
  • Ahn, Ik-Yong (Youngnam Flour Mills Co., LTD.) ;
  • Cho, Dong-In (Youngnam Flour Mills Co., LTD.) ;
  • Lee, Sang-Hyeon (Department of Bioscience and Biotechnology, Silla University)
  • Published : 2008.02.28
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Abstract

In order to obtain yeast cells producing a bacteriocin, Subpeptin JM4-A or Subpeptin JM4-B, the 48 bp oligonucleotides corresponding to Subpeptin JM4-A and Subpeptin JM4-B genes including codon for start and stop were chemically synthesized and cloned into pAUR123, an yeast expression vector. Transformed yeast cells exhibited growth inhibition of Bacillus subtilis, Escherichia coli and Pseudonomas aeruginosa. This result indicates that yeast cells producing Subpeptin JM4-A or Subpeptin JM4-B possess bacteriocidal properties against both Gram positive B. subtilis and Gram negative E. coli and P. aeruginosa cells. The recombinant yeast strains constructed in this study can be applied in the food preservative or. animal foodfeed.

박테리오신의 일종인 Bacillus subtilis의 Subpeptin JM4-A 및 Subpeptin JM4-B를 생산하는 효모의 제작을 위하여 48 bp 길이의 개시코돈과 종지코돈을 포함하는 Subpeptin JM4-A 및 Subpeptin JM4-B의 유전자를 합성하여 효모 발현 vector pAIR123에 클로닝하였다. 이렇게 제작된 재조합 DNA로 효모세포를 형질전환시켜 Subpeptin JM4-A와 Subpeptin JM4-B를 생산하는 형질전환 효모세포를 제작하였다. 형질전환 효모는 그람양성 대표세균인 고초균(B. subtilis)과 그람음성 장내세균인 대장균(E. coli)에 대해 항균활성을 나타냈다. 또한 농흉이나 중이염의 원인이 되는 녹농균(P. aeruginosa)에 대해서도 항균활성을 나타냈다. 이 연구의 결과로 부패하기 쉬운 식품들의 보존성을 향상시키기 위한 보존제 대체물질 또는 가축 사료에서 병원균의 생육을 저해하기 위한 항생제 대체물질로 사용할 수 있는 박테리오신을 산업적으로 생산할 수 있는 효모세포를 제작하였다.

Keywords

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