Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Study on Pandoraea sp. BCNU 315 Isolated from Soil

토양으로부터 분리한 Pandoraea sp. BCNU 315 에 관한 연구

  • Kim, Seon-A (Department of Biology, Changwon National University) ;
  • Choi, Hye-Jung (Interdisciplinary Program in Biotechnology, Changwon National University) ;
  • Woo, Seung-Hee (Interdisciplinary Program in Biotechnology, Changwon National University) ;
  • Hwang, Min-Jung (Department of Biology, Changwon National University) ;
  • Park, Mi-Ran (Interdisciplinary Program in Biotechnology, Changwon National University) ;
  • Kim, Dong-Wan (Department of Microbiology, Changwon National University) ;
  • Moon, Ja-Young (Department of Biochemistry and Health Sciences, Changwon National University) ;
  • Joo, Woo-Hong (Department of Biology, Changwon National University)
  • 김선아 (창원대학교 생물학과) ;
  • 최혜정 (창원대학교 생명공학협동과정) ;
  • 우승희 (창원대학교 생명공학협동과정) ;
  • 황민정 (창원대학교 생물학과) ;
  • 박미란 (창원대학교 생명공학협동과정) ;
  • 김동완 (창원대학교 미생물학과) ;
  • 문자영 (창원대학교 보건의과학과) ;
  • 주우홍 (창원대학교 생물학과)
  • Published : 2008.02.28
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Abstract

Bacteria that antagonize plant pathogenic fungi were isolated from the sediment soil at the Ansan industrial estate. One isolate of them showed growth inhibition of Rhizoctonia solani, Botrytis cenerea, and Fusarium oxysporum. This strain was identified as Pandoraea sp. based on phenotypic and phylogenetic characteristics and termed Pandoraea sp. BCNU 315. Tryptone as nitrogen source and sucrose as carbon source were found to be most effective for the microbial growth. In addition, the optimum temperature and pH for microbial growth were $30^{\circ}C$ and pH 7.0, respectively. The substances generated from Pandoraea sp. BCNU 315 were purified and analyzed by column chromatography, HPLC, GC-MS and NMR. As a result, one compound was determined to be indole, another compound was predicted as cyclopentadecaheptene. Detailed structural clarification of the all of the rest six compounds from Pandoraea sp. BCNU 315 has to be accompanied in the further studies.

안산지역의 하천 침전 토양에서 식물병원성진균에 대한 길항능력이 좋은 세균을 분리하였다. 이들 중 한 균주가 Rhizoctonia solani, Botrytis cenerea와 Fusarium oxysporum의 생장을 저해하는 활성을 보였다. 이 균주는 표현 형질과 분자계통학적인 특징에 의거하여 Pandoraea sp.로 동정되었다. 그리고 생육 최적 조건을 검토한 결과 tryptone과 sucrose가 각각 최적 질소원과 탄소원임을 확인할 수 있었으며, 최적 pH와 온도는 각각 pH 7.0 와 $30^{\circ}C$임이 확인되었다. Pandoraea sp. BCNU 315가 생성하는 물질들은 column chromatography로 정제하였고 고속액체크로마토그래피(HPLC), GC-MS 그리고 핵자기공명(NMR) 장치로 구조를 분석하였다. 그 결과 한 화합물은 indole이었으며 또 다른 화합물은 cyclopentadecaheptehe임이 추정되었다. 한편 Pandoraea sp. BCNU 315가 생성하는 6개의 화합물 중 나머지 4개 화합물의 자세한 구조 해석은 추후 연구를 통하여 밝혀져야 할 것이다.

Keywords

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