Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antioxidant Activities of the Solvent Extracts from Tetragonia tetragonioides

번행초 추출물의 항산화 활성

  • Lee, Min-A (Interdisciplinary Program in Biotechnology, Changwon National University) ;
  • Choi, Hye-Jung (Interdisciplinary Program in Biotechnology, Changwon National University) ;
  • Kang, Jum-Soon (School of Bioresource Science, Busan National University) ;
  • Choi, Young-Whan (School of Bioresource Science, Busan National University) ;
  • Joo, Woo-Hong (Department of Biology, Changwon National University)
  • 이민아 (창원대학교 대학원 생명공학협동과정) ;
  • 최혜정 (창원대학교 대학원 생명공학협동과정) ;
  • 강점순 (부산대학교 생명자원과학부) ;
  • 최영환 (부산대학교 생명자원과학부) ;
  • 주우홍 (창원대학교 생물학과)
  • Published : 2008.02.28
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Abstract

To clarify the antioxidation effect of the various solvent fractionation of Tetragonia tetragoniodes which has been known to superior plants for the traditional prevention and treatment of stomach-related diseases, total polyphenol and flavonoid contents, vitamin E content, the elimination activity of a DPPH free radical and superoxide anion, inhibition activity of the superoxide generation, reducing power and metal chelating effects were estimated. The contents of the polyphenol compounds were highest in the DCM and EA fractionation, and the content of flavonoid was high in the order of HX and EA fractionation. The vitamin E content showed high in the order of the HX and DCM fractionation among solvent fractions. $IC_{50}$ for the elimination effect of a DPPH radical were estimated as 554.25 and $394.96{\mu}g/ml$ in the DCM and EA fractions, respectively. These values were higher than that of BHT $784.7{\mu}g/ml$ widely used in antioxidation effect. The inhibition activity of the superoxide generation using the T. tetragoniodes solvent fractions represented the effects similar to that of ${\alpha}$-tocopherol known to prevent the lipoprotein oxidation, but lower consequences than that of the phenol-resins, BHT and BHA, respectively. In the antioxidation activity derived by the reducing capability, the EA fractionation in a 1.5 mg/ml concentration showed higher than that of ${\alpha}$-tocopherol.

본 연구에서는 예로부터 위장병의 예방 및 치료 효과가 뛰어난 작물로 알려진 번행초(Tetragonia tetragonioides)의 각종 용매 추출 분획물의 항산화 효과를 명확히 하기 위해 총 폴리페놀과 플라보노이드 함량, 비타민 E함량, DPPH free radical 소거활성, Superoxide 음이온 소거활성, 과산화물생성 억제 활성, 환원능력 그리고 금속 킬레이트 효과를 측정하였다. 먼저 총 폴리페놀 화합물의 함량은 DCM 분획, EA 분획에서 높게 나타났고, 플라보노이드 함량은 HX 분획, EA 분획 순으로 나타났다. 비타민 E의 함량은 HX 분획, DCM 분획 순으로 높게 나타났다. DPPH radical 소거활성 효과는 DCM과 EA 추출 분획에서 $IC_{50}$ 값이 각각 $554.25{\mu}g/ml,\;394.96{\mu}g/ml$으로 산화방지제로 널리 이용되고 있는 BHT의 $784.7{\mu}g/ml$보다 우수하게 측정되었다. 과산화물 생성 억제 활성에서 번행초 분획은 지질단백질을 산화로부터 보호한다고 알려진 ${\alpha}$-tocopherol과 유사한 능력을 보였으나, 페놀계 합성 항산화제인 BHT, BHA에 비해서는 억제능이 다소 낮음이 확인되었다. 환원능력을 통한 항산화 활성 측정에서는 1.5 mg/ml농도에서 EA 분획의 활성이 ${\alpha}$-tocopherol보다 유의하게 높게 나타나 좋은 항산화 소재임이 확인되었다.

Keywords

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