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Arabidopsis nucleoside diphosphate kinase-2 as a plant GTPase activating protein

  • Shen, Yu (Department of Chemistry, University of Nebraska-Lincoln) ;
  • Han, Yun-Jeong (Department of Molecular Biotechnology and Kumho Life Science Laboratory, Chonnam National University) ;
  • Kim, Jeong-Il (Department of Molecular Biotechnology and Kumho Life Science Laboratory, Chonnam National University) ;
  • Song, Pill-Soon (Department of Chemistry, University of Nebraska-Lincoln)
  • Published : 2008.09.30
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Abstract

Nucleoside diphosphate kinase (NDPK) is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Arabidopsis NDPK2, like its mammalian counterparts, is multifunctional despite its initial discovery phytochrome-interacting protein. This similarity raises the possibility that NDPK2 may play a role in G-protein signaling in plants. In the present study, we explore the potential relationship between NDPK2 and the small G proteins, Pra2 and Pra3, as well as the heterotrimeric G protein, GPA1. We report a physical interaction between NDPK2 and these small G proteins, and demonstrate that NDPK2 can stimulate their GTPase activities. Our results suggest that NDPK2 acts as a GTPase-activating protein for small G proteins in plants. We propose that NDPK2 might be a missing link between the phytochrome-mediated light signaling and G protein-mediated signaling.

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References

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