Evaluation of the Various Artificial Skin Substitutes Implanted onto Nude Mice

누드마우스를 이용한 다양한 피부 대체물의 성능비교

  • Lee, Won Jai (Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine) ;
  • Lee, Dong Won (Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine) ;
  • Hur, Jae Young (Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine) ;
  • Lee, Young Dae (Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine) ;
  • Park, Beyoung Yun (Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine) ;
  • Rah, Dong Kyun (Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine)
  • 이원재 (연세대학교 의과대학 성형외과학교실, 인체조직복원연구소) ;
  • 이동원 (연세대학교 의과대학 성형외과학교실, 인체조직복원연구소) ;
  • 허재영 (연세대학교 의과대학 성형외과학교실, 인체조직복원연구소) ;
  • 이영대 (연세대학교 의과대학 성형외과학교실, 인체조직복원연구소) ;
  • 박병윤 (연세대학교 의과대학 성형외과학교실, 인체조직복원연구소) ;
  • 나동균 (연세대학교 의과대학 성형외과학교실, 인체조직복원연구소)
  • Received : 2007.08.30
  • Published : 2008.03.10

Abstract

Purpose: The purpose of this study is to evaluate the remodeling process of the various skin substitutes in 4th and 6th weeks following the transplantation when transplanted onto nude mice. Methods: Three types of artificial skin substitutes, such as PLGA scaffold with keratinocyte sheets(group 1), acellular human dermis($Surederm^{(TM)}$) and keratinocyte sheet(group 2), bioengineered skin($Neoderm^{(TM)}$)(group 3), were applied to the wound on nude mice. All mice were killed in 2, 4 weeks and/or 6 weeks after grafting and tissue samples were harvested from the back of mice. The changes in wound size, degree of angiogenesis, formation of basement membrane and epidermis, density of collagen fibers and neural restoration were examined. Results: There was no significant changes in wound size among the three groups. However, the size of wound decreased in the non-substituted group due to contracture. Degree of angiogenesis and systhesis of collagen or neurofilaments were mostly increased in bioengineered skin($Neoderm^{(TM)}$)(group 3), followed by acellular human dermis($Surederm^{(TM)}$) and keratinocyte sheet(group 2), PLGA scaffold with keratinocyte sheets (group 1). However, group 3 and group 2 showed similar thickness of basement membrane and epidermis. Conclusion: We found that degree of angiogenesis, formation of basement membrane and skin appendages, density of collagen fibers and neurofilaments can be the categories to evaluate the success of artificial skin substitution in early stages.

Keywords

References

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