Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

Characterization and pathogenicity of extracellular products (ECPs) of Vibrio harveyi

Vibrio harveyi에서 분리한 extracellular products (ECPs)의 특성과 독성

  • Choi, Jeong-Hyun (East Sea Mariculture Research Center, National Fisheries Research & Development Institute) ;
  • Won, Kyoung-Mi (Institute of Fisheries Science, Pukyong National University) ;
  • Ha, Su-Jin (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Yi-Cheong (East Sea Mariculture Research Center, National Fisheries Research & Development Institute) ;
  • Byun, Soon-Gyu (East Sea Mariculture Research Center, National Fisheries Research & Development Institute) ;
  • Lee, Bae-Ik (East Sea Mariculture Research Center, National Fisheries Research & Development Institute) ;
  • Lee, Jong-Ha (East sea Fisheries Research Institute, NFRDI) ;
  • Huh, Min-Do (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Park, Su-Il (Department of Aquatic Life Medicine, Pukyong National University)
  • 최정현 (국립수산과학원 동해특성화연구센터) ;
  • 원경미 (부경대학교 수산과학연구소) ;
  • 하수진 (부경대학교 수산생명의학과) ;
  • 김이청 (국립수산과학원 동해특성화연구센터) ;
  • 변순규 (국립수산과학원 동해특성화연구센터) ;
  • 이배익 (국립수산과학원 동해특성화연구센터) ;
  • 이종하 (국립수산과학원 동해수산연구소) ;
  • 허민도 (부경대학교 수산생명의학과) ;
  • 박수일 (부경대학교 수산생명의학과)
  • Published : 20080800
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Abstract

Vibrio harveyi, one of the major causal agent of vibriosis, affects a diverse range of marine vertebrates and invertebrates over a wide geographical area. The aims of this study were to investigate the characteristics of extracellular products (ECPs) from an isolate of the pathogenic non‐luminous V. harveyi and the luminous V. harveyi. And ECPs of V. harveyi were examined the pathogenicity to the black rockfish, Sebastes schlegeli, and histopathological traits of internal organs injected by ECPs. Four strains of V. harveyi cultures produced ECPs showing various enzymatic activites (caseinase, gelatinase, phospholipase, lipase, haemolysin). The ECPs showed strong cytotoxicity on macrophages of black rockfish, Sebastes schlegeli and olive flounder, Paralichthys olivaceus. Especially, the virulence of ECPs from the isolate of V. harveyi FR 2 was higher in the intraperitoneally injected black rockfish. Also, the ECPs of V. harveyi FR 2 caused the expansion of sinusoids in the liver, the activation of ellipsoid in the spleen and the sloughing of the epidermal cell in the intestine. It was suggested that the ECPs from V. harveyi play an important role in the pathogenicity process of the V. harveyi.

Keywords

References

  1. Alvarez, J.J., Austin, B., Alvarez, A.M. and Reyes, H.: Vibrio harveyi: a pathogen of penaeid shrimps and fish in Venezuela. J. Fish Dis., 21: 313-316, 1998 https://doi.org/10.1046/j.1365-2761.1998.00101.x
  2. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem., 72: 248-254, 1976 https://doi.org/10.1016/0003-2697(76)90527-3
  3. Choi, J.H., Won, K.M., Sohn, S.B., Park, H.J., Byun, S.G., Lee, B.I., Lee, J.H., Kim, Y.C. and Park, S.I.: Pathogenicity of Vibrio harveyi to black rockfish, Sebastes schlegeli. J. Fish Pathol., 20: 99-108, 2007
  4. Garcia Moreno M.L. and Landgraf, M.: Virulence factors and pathogenicity of Vibrio vulnificus strains isolated from seafood. J. Appl. Microbiol., 84: 747-751, 1998 https://doi.org/10.1046/j.1365-2672.1998.00404.x
  5. Henke, J.M. and Bassler, B.L.: Quorum sensing regulates type III secretion in Vibrio harveyi and Vibrio parahaemolyticus. J. Bacteriol.,186: 3794-3805, 2004 https://doi.org/10.1128/JB.186.12.3794-3805.2004
  6. Ishimaru, K. and Muroga, K.: Taxonomical re examination of two pathogenic Vibrio species isolated from milkfish and swimming crab. Fish Pathol., 32: 59-64, 1997 https://doi.org/10.3147/jsfp.32.59
  7. Jiravanichpaisal P., Miyazaki T. and Iimsuwan C.: Histopathology, biochemistry and pathogenicity of Vibrio harveyi infecting black tiger prawn Penaeus monodon. J. Aquat. Anim. Health., 6:27-35, 1994 https://doi.org/10.1577/1548-8667(1994)006<0027:HBAPOV>2.3.CO;2
  8. Kraxberger Beatty, T., McGarey, D.J., Grier, H.J. and Lim, D.V.: Vibrio harveyi, an opportunistic pathogen of common snook, Centropomus undecimalis (Bloch), held in captivity. J. Fish Dis., 13: 557-560, 1990 https://doi.org/10.1111/j.1365-2761.1990.tb00819.x
  9. Lamas, J., Santos, Y., Bruno, D.W., Toranzo, A.E. and Anadon, R.: Non specific cellular responses of rainbow trout to Vibrio anguillarum and its extracellular products (ECPs). J. Fish Biol., 45: 839-854, 1994 https://doi.org/10.1111/j.1095-8649.1994.tb00949.x
  10. Lavilla Pitogo, C.R., Baticados, M.C.L., Cruz Lacierda, E.R. and de la Pena, L.D.: Occurrence of luminous bacterial disease of Penaeus monodon larvae in the Philippines. Aquaculture, 91: 1-13, 1990 https://doi.org/10.1016/0044-8486(90)90173-K
  11. Lee, K.K., Liu, P.C. and Chuang, W.H.: Pathogenesis of gastroenteritis caused by Vibrio carchariae in cultured marine fish. Mar. Biotechnol., 4: 267-277, 2002 https://doi.org/10.1007/s10126-002-0018-9
  12. Liu, P.C., Lee, K.K., Yii, K.C., Kou, G.H. and Chen, S.N.: Isolation of Vibrio harveyi from diseased kuruma prawns Penaeus japonicus. Curr. Microbiol., 33: 129-132, 1996a https://doi.org/10.1007/s002849900087
  13. Liu, P.C., Lee, K.K. and Chen, S.N.: Pathogenicity of different isolates of Vibrio harveyi in tiger prawn, Penaeus monodon. Lett. Appl. Microbiol., 22: 413-416, 1996b https://doi.org/10.1111/j.1472-765X.1996.tb01192.x
  14. Liu, P.C. and Lee, K.K.: Cysteine protease is a major exotoxin of pathogenic luminous Vibrio harveyi in the tiger prawn, Penaeus monodon. Letters Appl. Microbiol., 28: 428-430, 1999 https://doi.org/10.1046/j.1365-2672.1999.00555.x
  15. Liu, P. C., Chuang, W. H. and Lee, K. K.: Infectious gastroenteritis caused by Vibrio harveyi (V. carchariae) in cultured red drum, Sciaenops ocellatus. J. Appl. Ichthyol., 19: 59-61, 2003 https://doi.org/10.1046/j.1439-0426.2003.00356.x
  16. Madetoja, J., Pylkko, P., Pohjanviyta, T., Schildt, L. and Pelkonen, S.: Putative virulaence factors of atypical Aeromonas salmonicida isolated from Arctic charr, Salvelinus alpinus (L.), and European grayling, Thymallus thymallus (L.). J. Fish Dis., 26: 349-359, 2003 https://doi.org/10.1046/j.1365-2761.2003.00470.x
  17. Montero, A. B. and Austin B.: Characterization of extracellular products from an isolate of Vibrio harveyi recovered from diseased post larval Penaeus vannamei (Bonne). J. Fish Dis., 22: 377-386, 1999 https://doi.org/10.1046/j.1365-2761.1999.00189.x
  18. Nishimori, E., Hasegawa O., Numata T. and Wakabayashi H.: Vibrio carchariae causes mass mortalities in Japanese abalone, Sulculus diversicolor supratexta. Fish Pathol., 33: 495-502, 1998 https://doi.org/10.3147/jsfp.33.495
  19. Oakey, H.J. and Owens, L.: A new bacteriophage, VHML, isolated from a toxin producing strain of Vibrio harveyi in tropical Australia. J. Appl. Microbiol., 89: 702-709, 2000 https://doi.org/10.1046/j.1365-2672.2000.01169.x
  20. Pasharawipas, T., Thaikua, S., Sriurairatana, S., Ruangpan, L., Direkbusarakum, S., Manopviset charean, J. and and Flegel, T.W.: Partial characterization of a novel bacteriophage of Vibrio harveyi isolated from shrimp culture ponds in Thailand. Virus Res., 114: 63-69, 2005 https://doi.org/10.1016/j.virusres.2005.05.012
  21. Soto Rodriguez, S.A., Roque, A., Lizarranga Partida, M.L., Guerra Flores, A.L. and Bomez Gil, B.: Virulence of luminous vibrios to Artemia franciscana nauplii. Dis. Aquat. Org., 53: 231-240, 2003 https://doi.org/10.3354/dao053231
  22. Sunaryanto, A. and Mariam, A.: Occurrence of a pathogenic bacteria causing luminescence in penaeid larvae in Indonesian hatcheries. Bull. Brackishwater Aquacult. Devel. Cen., 8: 105-112, 1986
  23. Suprapto, H., Hara, T., Nakai, T. and Muroga, K.: Purification of a lethal toxin of Edwardsiella tarda. Fish Pathol., 31: 203-207, 1996 https://doi.org/10.3147/jsfp.31.203
  24. Wang, X.H., Oon, H,L., Ho, G.W.P., Wong, W.S., Lim, F.T.M. and Leung. K.Y.: Internalization and cytotoxicity are important virulence mechanisms in vibrio fish epithelial cell interactions. Microbiol., 144: 2987-3002, 1998 https://doi.org/10.1099/00221287-144-11-2987
  25. Won, K.M., Kim, S.M. and Park, S.I.: Characterization of Vibrio harveyi, the causal agent of vibriosis in cultured marine fishs in Korea. J. Fish Sci. Technol., 9: 123-128, 2006 https://doi.org/10.5657/fas.2006.9.3.123
  26. Yamada, Y. and Wakabayshi, H.: Identification of fish pathogenic strains belonging to the genus Edwardsiella by sequence analysis of sodB. Fish Pathol., 34: 145-150, 1999 https://doi.org/10.3147/jsfp.34.145
  27. Zhang, X.H. and Austin, B.: Pathogenicity of Vibrio harveyi to salmonids. J. Fish Dis., 23: 93-102, 2000 https://doi.org/10.1046/j.1365-2761.2000.00214.x
  28. Zorrilla, I., Arijo, S., Chabrillon, M., Diaz, P., Martinez Manzanares, E., Balebona, M. C. and Morinigo, M.A.: Vibrio species isolated from diseased farmed sole, Solea senegalensis (Kaup), and evaluation of the potential virulence role of their extracellular products. J. Fish Dis., 26: 103-108, 2003 https://doi.org/10.1046/j.1365-2761.2003.00437.x
  29. 醫化學硏究所學友會: 細菌學實習提要. pp 566-568. (In Japanese), 1976