The Korean Journal of Pain

The Korean Pain Society (대한통증학회)
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Spinal α2 Adrenoceptor and Antiallodynic Effect by Clonidine after Chronic Administration of 4-Methylcatechol in Neuropathic Rat Pain Model

신경병증성 통증모델에서 신경영양인자 유도물질의 반복 투여가 척수 아드레날린계에 미치는 영향

  • Chung, Kyu Yeon (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) ;
  • Shin, Sang Wook (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) ;
  • Choi, Bong Soo (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) ;
  • Kim, Chul Hong (Department of Anesthesia and Pain Medicine, School of Dentistry, Pusan National University) ;
  • Kim, Kyung Hoon (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) ;
  • Kim, Hae Kyu (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University)
  • 정규연 (부산대학교 의학전문대학원 마취통증의학교실) ;
  • 신상욱 (부산대학교 의학전문대학원 마취통증의학교실) ;
  • 최봉수 (부산대학교 의학전문대학원 마취통증의학교실) ;
  • 김철홍 (부산대학교 치의학전문대학원 마취통증의학교실) ;
  • 김경훈 (부산대학교 의학전문대학원 마취통증의학교실) ;
  • 김해규 (부산대학교 의학전문대학원 마취통증의학교실)
  • Received : 2008.08.14
  • Accepted : 2008.11.03
  • Published : 2008.12.30
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Abstract

Background: The adrenergic nervous system in the spinal cord contributes to the development of neuropathic pain after nerve injury. Brain derived neurotrophic factor may facilitate the sympathetic change in the spinal cord and influence the state of neuropathic pain. We probed the effect of chronic repetitive administration of systemic 4-methylcatechol, which is known to be a neurotrophic factor inducer, in a spinal nerve ligation model. Methods: We made the rat neuropathic pain model by the ligation of the L5 spinal nerve. Intraperitoneal 4-methylcatechol ($10{\mu}g/kg$) or the same volume of saline wasadministrated twice daily just after the operation for 7 days. The tactile allodynia was measured by using von Frey filaments and its change was followed up from 3 days after SNL. The lumbosacral enlargement of the spinal cord was taken out and the mRNA contents of the ${\alpha}_2-adrenoceptor$ subtypes were measured by real time polymerase chain reaction and this was then compared with the control groups. The antiallodynic effect of intrathecal clonidine (3, 10, $30{\mu}g$) was evaluated and compared in the 4-methylcatechol treated rats and the control rats. Results: The expression of the ${\alpha}_{2A}$ and ${\alpha}_{2C}$ adrenoceptor subtypes did not change after 4-methylcatechol treatment. Intrathecal clonidine showed an earlier and better effect at the highest dose ($30{\mu}g$ intrathecal), but not with any other doses. Conclusions: Chronic intraperitoneal administration of 4-methylcatechol may improve the effect of intrathecal clonidine, but we could not prove the increase of ${\alpha}_{2A}$ and ${\alpha}_{2C}$ adrenoceptors in the spinal cord of 4-methylcatechol treated rats.

Keywords

Acknowledgement

Supported by : 부산대학교병원 의학연구소

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