Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Repression of Transcriptional Activity of Estrogen Receptor α by a Cullin3/SPOP Ubiquitin E3 Ligase Complex

  • Byun, Boohyeong (Department of Oriental Medicine, Daegu Haany University) ;
  • Jung, Yunhwa (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
  • Received : 2007.08.04
  • Accepted : 2007.10.21
  • Published : 2008.04.30
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Abstract

The role of SPOP in the ubiquitination of $ER{\alpha}$ by the Cullin3-based E3 ubiquitin ligase complex was investigated. We showed that the N-terminal region of SPOP containing the MATH domain interacts with the AF-2 domain of $ER{\alpha}$ in cultured human embryonic 293 cells. SPOP was required for coimmunoprecipitation of $ER{\alpha}$ with Cullin3. This is the first report of the essential role of SPOP in $ER{\alpha}$ ubiquitination by the Cullin3-based E3 ubiquitin ligase complex. We also demonstrated repression of the transactivation capability of $ER{\alpha}$ in cultured mammalian cells.

Keywords

References

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