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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Expression of Gal4-VP16 and Gal4-DNA binding domain under the control of the T lymphocyte-specific lck proximal promoter in transgenic mice

  • Ryu, Chun-Jeih (Institute of Bioscience, Department of Bioscience and Biotechnology, Sejong University) ;
  • Whitehurst, Charles E. (Center for Cancer Research and Department of Biology, Massachusetts Institutes of Technology) ;
  • Chen, Jianzhu (Center for Cancer Research and Department of Biology, Massachusetts Institutes of Technology)
  • Published : 2008.08.31
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Abstract

Thymocyte-specific transcriptional regulatory systems can be used to better understand the relationship between transcription and V(D)J recombination during early T cell development. In this study, we generated transgenic mice expressing the transactivator Gal4-VP16 or the Gal4 DNA binding domain (Gal4-DBD) under the control of the lck proximal promoter, which is only active in immature thymocytes. From these studies Gal4-VP16 and Gal4-DBD expression was shown to significantly alter thymic cellularity and differentiation without significantly changing the $CD3^+$ thymocyte distribution. Furthermore, the presence of Gal4-VP16 or Gal4-DBD in the transgenic thymocytes retarded the mobility of the Gal4 DNA binding motif as determined by a gel mobility shift assay, suggesting that the developmental alteration did not affect the functional property of the transgenic proteins. These results indicated that lck promoter-driven Gal4-VP16 or Gal4-DBD expression did not affect $CD3^+$ mature thymocytes, thus this system can be applied to study transcriptional regulation of transresponder genes in bigenic mouse model thymocytes.

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References

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