Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Application of Open-type Liquid Culture for Large-scale Production of Mature Plus Tree of Eucalyptus pellita

개방형 액체대량배양 시스템을 통한 유칼리나무 펠리타 선발목의 대량증식

  • Park, So-Young (Biotechnology Division, Korea Forest Research Institute) ;
  • Moon, Heung-Kyu (Biotechnology Division, Korea Forest Research Institute) ;
  • Kim, Yong-Wook (Biotechnology Division, Korea Forest Research Institute) ;
  • Kim, Seon-Ja (Biotechnology Division, Korea Forest Research Institute) ;
  • Yi, Jae-Seon (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University)
  • 박소영 (국립산림과학원 생물공학과) ;
  • 문홍규 (국립산림과학원 생물공학과) ;
  • 김용욱 (국립산림과학원 생물공학과) ;
  • 김선자 (국립산림과학원 생물공학과) ;
  • 이재선 (강원대학교 산림자원학부)
  • Received : 2008.09.30
  • Accepted : 2008.10.24
  • Published : 2008.12.30
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Abstract

In an attempt to establish the mass proliferation system, Eucalyptus pellita, a 5-year-old plus tree, was cultured with three different culture types in 1L vessels: solid culture without ventilation (conventional culture), liquid culture without ventilation and open-type liquid culture with forced ventilation. Then the culture scale was subsequently increased from 1L to 10L in vessel volume. After 4 weeks of 1L-scale culture, the best growth was obtained by culturing plantlets on open-type liquid culture, suggesting that the in vitro plantlets growth can be enhanced by liquid medium and ventilation. In open-type large scale culture in 10L vessel, plantlets growth resulted in a 370% increase in the number of nodes, 3.6 times increase in leaf expansion, and 3.3 times increase in shoot length, while the conventional culture suppressed shoot growth due to the callusing on the leaves and lack of $CO_2$. The results indicated that the open-type large scale culture system was effective for enhancing productivity by improving growth of the plantlets in clonally proliferated plus tree, Eucalyptus pellita.

유칼리나무 펠리타(Eucalyptus pellita) 선발목(5년생)의 기내 대량증식을 위해 1L 규모로 1) 고체배양(대조구), 2) 액체정치배양, 3) 개방형 액체배양 등 3가지 배양법이 시도 되었고, 그 결과를 토대로 배양규모를 10L로 확대하여 대량배양 가능성을 조사하였다. 1L 배양용기에서 4주간 배양한 결과, 공기가 공급 된 개방형 액체배양에서 가장 좋은 생장 결과를 얻었다. 이는 액체배지에 의한 원활한 염류 공급과 배양용기내 공기의 환기에 의해 얻어진 결과로 생각된다. 배양 규모를 10L로 확대하여 액체대량배양을 실시한 결과, 식물체의 생장은 대조구인 고체배양에 비교하여 마디 수에 있어서 370%, 엽 면적 3.6배, 그리고 신초 길이 3.3배 신장하는 결과를 보였다. 이는 개방형 액체대량배양 시스템이 유칼리나무 클론증식시 생산성을 향상시키는데 적합한 시스템임을 보여주는 결과라고 하겠다.

Keywords

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