Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Microarray Analysis of the Gene Expression Profiles of SL2 Cells Stimulated by LPS/PGN and Curdlan

  • Jin, Li Hua (Department of Biochemistry, Yonsei University) ;
  • Choi, Jung Kyoon (Department of Biochemistry, Yonsei University) ;
  • Cho, Hwan Sung (Department of Biochemistry, Yonsei University) ;
  • Shim, Jaewon (Department of Biochemistry, Yonsei University) ;
  • Kim, Young-Joon (Department of Biochemistry, Yonsei University)
  • Received : 2007.11.02
  • Accepted : 2007.12.13
  • Published : 2008.06.30
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Abstract

Essential aspects of the innate immune response to microbial infection appear to be conserved between insects and mammals. In order to identify new Drosophila melanogaster genes involved in the immune response, we performed gene expression profiling of Drosophila SL2 cells stimulated with bacterial (LPS/PGN) or fungal (curdlan) components using a cDNA microarray that contained 5,405 Drosophila cDNAs. We found that some genes were similarly regulated by LPS/PGN and curdlan. However, a large number, belonging to the functional classes of cell organization, development, signal transduction, morphogenesis, cell cycle, and DNA replication, displayed significant differences in their transcription profiles between the two treatments, demonstrating that bacterial and fungal components induce different immune response even in an in vitro cell system.

Keywords

Acknowledgement

Supported by : Ministry of Science and Technology

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