Molecules and Cells

  • 제25권4호
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  • Pages.487-493
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  • 2008
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
권호 표지

Forced Expression of HoxB4 Enhances Hematopoietic Differentiation by Human Embryonic Stem Cells

  • Lee, Gab Sang (Moore Laboratory, Cell Biology Program, Memorial Sloan-Kettering Cancer Center) ;
  • Kim, Byung Soo (Moore Laboratory, Cell Biology Program, Memorial Sloan-Kettering Cancer Center) ;
  • Sheih, Jae-hung (Moore Laboratory, Cell Biology Program, Memorial Sloan-Kettering Cancer Center) ;
  • Moore, Malcolm AS (Moore Laboratory, Cell Biology Program, Memorial Sloan-Kettering Cancer Center)
  • 투고 : 2007.09.05
  • 심사 : 2008.02.11
  • 발행 : 2008.06.30
⟨ 이전 논문 다음 논문 ⟩

초록

HoxB4 has been shown to enhance hematopoietic engraftment by hematopoietic stem cells (HSC) from differentiating mouse embryonic stem cell (mESC) cultures. Here we examined the effect of ectopic expression of HoxB4 in differentiated human embryonic stem cells (hESCs). Stable HoxB4-expressing hESCs were established by lentiviral transduction, and the forced expression of HoxB4 did not affect stem cell features. HoxB4-expressing hESC-derived CD34+ cells generated higher numbers of erythroid and blast-like colonies than controls. The number of CD34+ cells increased but CD45+ and KDR+ cell numbers were not significantly affected. When the hESC derived CD34+ cells were transplanted into $NOD/SCID{\beta}2m-/-$ mice, the ectopic expression of HoxB4 did not alter their repopulating capacity. Our findings show that overexpression of HoxB4 in differentiating hESCs increases hematopoietic colony formation and hematopoietic cell formation in vitro, but does not affect in vivo repopulation in adult mice hosts.

키워드

과제정보

연구 과제 주관 기관 : Ministry of Science and Technology

참고문헌

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