한국어병학회지 (Journal of fish pathology)

  • 제21권3호
  • /
  • Pages.209-217
  • /
  • 2008
  • /
  • 1226-0819(pISSN)
  • /
  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

양식 강도다리, Platichthys stellatus Pallas에서 분리된 Streptococcus parauberis의 특성

Isolation of Streptococcus parauberis from starry flounder, Platichthys stellatus Pallas

  • 조미영 (국립수산과학원 병리연구과) ;
  • 이재일 (포항수산사무소) ;
  • 김명석 (국립수산과학원 병리연구과) ;
  • 최희정 (국립수산과학원 병리연구과) ;
  • 이덕찬 (국립수산과학원 병리연구과) ;
  • 김진우 (국립수산과학원 병리연구과)
  • Cho, Mi-Young (Pathology Division, National Fisheries Research and Development Institute) ;
  • Lee, Jae-Il (Pohang Fisheries Office, NFRDI) ;
  • Kim, Myoung-Sug (Pathology Division, National Fisheries Research and Development Institute) ;
  • Choi, Hee-Jung (Pathology Division, National Fisheries Research and Development Institute) ;
  • Lee, Deok-Chan (Pathology Division, National Fisheries Research and Development Institute) ;
  • Kim, Jin-Woo (Pathology Division, National Fisheries Research and Development Institute)
  • 발행 : 20081200
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A disease with mass mortality characterized exophthalmia and abdominal distension by ascites occurred in farmed starry flounder, Platichthys stellatus in Pohang since October of 2007. Severe necrosis and infiltration of inflammatory cells were observed in tissue histopathological sections from liver, kidney, spleen and heart of the diseased fish. The catalase-negative and gram positive cocci with -hemolysis were isolated from moribund fish. Analysis of the API profiles and the multiplex PCR of the isolates showed that strains were identified as Streptococcus parauberis. In API profiles, S. parauberis isolated from starry flounder differed from those of olive flounder, Paralichthys olivaceus to the test of arginine dihydrolase, D-mannitol, D-sorbitol and α-lactose. And the strains, PH0710 and PH0711, were strongly lethal for starry flounder (LD50 ranging from 4.2×103~4.2×102 cfu/fish at 7 days after intraperitoneally inoculation). This is the first report on a S. parauberis infection in starry flounder.

키워드

참고문헌

  1. Austin, B. and Austin, D.A. (1999): Gram positive bacteria-the lactic acid bacteria. In Bacteria Fish Pathogens. Diseases in Farmed and Wild Fish, 3th ed., Springer, UK., pp. 38-49
  2. Baeck, G.W., Kim, J.H., Gomez, D.K. and Park, S.C. (2006): Isolation and characterization of Streptococcus sp. from diseased flounder (Paralichthys olivaceus) in Jeju island. J. Vet. Sci., 7: 53-58 https://doi.org/10.4142/jvs.2006.7.1.53
  3. Domenech, A., Ferenandez-Garayzabal, J.F., Pascual, C., Garcia, J.A., Cutuli, M.T., Moreno, M.A., Collins, M.D. and Dominguez, L. (1996): Streptococcosis in cultured turbot, Scophthalmus maximus (L.), associated with Streptococcus parauberis. J. Fish Dis., 19: 33-38 https://doi.org/10.1111/j.1365-2761.1996.tb00117.x
  4. Elder, A., Goria, M., Ghittino, C., Zlotikin, A. and Bercovier, H. (1999): Biodiversity of Lactococcus garvieae strains isolated fish in Europe, Asia, and Australia. Appl. Environ. Microbiol., 65: 1005-1008
  5. Kitao, T. (1993): Streptococcal infection. In Bacterial Diseases of Fish, Inglis, V., Roberts, R.J. and Bromage, N.R. (Editors), Blackwell, Oxford, pp. 196-210
  6. Kramer, D.E., Barss, W.H., Paust, B.C. and Bracken, B.E. (1995): Guide to northeast pacific flatfishes: families Bothidae, Cynoglossidae, and Pleuronectidae. Marine Advisory Bulletin, 47: 1-104
  7. Kusuda, R. and Salati, F. (1993): Major bacterial diseases affecting mariculture in Japan. Annu. Rev. Fish Dis., 3: 9-85
  8. Miyazaki, T., Kubota, S.S., Kaige, N. and Miyashita, T. (1984): A histopathological study of streptococcal disease in tilapia. Fish Pathol., 19: 167-172 https://doi.org/10.3147/jsfp.19.167
  9. Nieto, J.M, Devesa, S., Quoroga, I. and Toranzo, A.E. (1995): Pathology of Enterococcus sp. infection in farmed turbot, Scophthalmus maximus L. J. Fish Dis., 18: 21-30 https://doi.org/10.1111/j.1365-2761.1995.tb01262.x
  10. Orcutt, H.G. (1950): The life history of the starry flounder Platichthys stellatus (Pallas). Calif. Fish Bull., 78: 25-32
  11. Romalde, J.L., Magarinos, B., Villar, C., Barja, J.L. and Toranzo, A.E. (1999): Genetic analysis of turbot pathogenic Streptococcus parauberis strains by ribotyping and random amplified polymorphic DNA. FEMS Microbiol. Lett., 459: 297-304
  12. 강철영, 강봉조, 문영건, 김기영, 허문수 (2007): 제주도 양식넙치 (Paralichthys olivaceus)로부터 분리한 비 용혈성 연쇄구균의 동정. 한국어병학회지, 20: 109-117
  13. 권문경, 임한규, 민병화, 변순규, 김이청, 조병열 (2007): 양식환경이 강도다리, Platichthys stellatus의 혈액 생화학적 성상 및 lysozyme 활성에 미치는 영향. 한국어병학회지, 20: 281-289
  14. 우승호, 김현정, 이주석, 김진우, 박수일 (2006): 해수 양식어류에서 분리된 연쇄상구균의 종류와 병원성. 한국어병학회지, 19: 17-33
  15. 이창훈, 김필연, 고창식, 오덕철, 강봉조 (2007): 제주지역 양식 넙치 (Paralichthys olivaceus)로부터 분리되는 Streptococcus iniae와 Streptococcus parauberis의 생물학적특성. 한국어병학회지, 20: 33-40
  16. 전세규 (2005): 넙치의 질병과 치료, 한국수산신문사, 서울, pp. 108-110
  17. 정용욱, 강봉조, 박근태, 허문수 (2004): 16S-23S rRNA intergenic spacer region을 이용한 어류병원성 Streptococcus iniae의 분자생물학적 동정. 한국어병학회지, 17: 91-98
  18. 조미영, 김명석, 권문경, 지보영, 최혜승, 최동림, 박경현, 이창훈, 김진도, 이주석, 오윤경, 이덕찬, 박신후, 박명애 (2007a): 2005년부터 2006년 사이 우리나라 양식넙치, Paralichthys olivaceus의 세균성 질병에 대한역학조사. 한국어병학회지, 20: 61-70
  19. 조미영, 오윤경, 이덕찬, 김재훈, 박명애 (2007b): 양식 넙치에서 분리한 Streptococcus parauberis의 동정방법에 따른 지역적 비교. 한국어병학회지, 20:49-60
  20. 조미영, 이덕찬, 최희정, 강봉조, 정승희, 김진우 (2006): 양식넙치, Paralichthys olivaceus에서 분리한 Streptococcus parauberis의 병원성. 2006년도 수산관련학회 공동학술대회 발표요지집, pp. 516-517