Karyotype of Dwarf Loach, Kichulchoia brevifasciata (Pisces: Cobitidae) from Korea

미꾸리과 소형어류 좀수수치, Kichulchoia brevifasciata의 핵형

  • Kim, Ik-Soo (Faculty of Biological Sciences, College of Natural Sciences, Chonbuk National University) ;
  • Kim, Eun-Jin (Faculty of Biological Sciences, College of Natural Sciences, Chonbuk National University)
  • Received : 2008.02.12
  • Accepted : 2008.03.11
  • Published : 2008.03.31

Abstract

The karyotype analysis of Kichulchoia brevifasciata was carried out, known as an end-angered small endemic cobitid fish of Korea. Five females and one male were collected from Geum-san-myeon, Goheung-gun, Jeollanam-do, Korea and its gill, kidney and digestive tract tissues were treated by flame-drying technique. The karyotype was found to be 2n= 48 from 33 cells; 16 meta-submetacentric and 32 subtelo-telocentric chromosomes; 64 FN (fundamental number). This karyotype showed a remarkably different karyotype formula from Niwaella multifasciata which is closely related to this species.

멸종위기에 처한 한국 고유종인 미꾸리과(Cobitidae) 소형어류 좀수수치, Kichulchoia brevifasciata의 핵형을 분석하였다. 전라남도 고흥군 금산면에서 6개체를 채집하여 핵형분석 결과 염색체 수는 2n=48 으로서 16 meta-submetacentric, 32 subtelo-telocentric chromosomes로 구성되어 있었으며 FN=64 이었고 유연관계에 있는 수수미꾸리, Niwaella multifasciata와 핵형과 구성에 있어 큰 차이를 나타냈다.

Keywords

References

  1. Arai, R. 1983. Karyological and osteological approach to phylogenetics of fishes. Bull. Nat. Sci. Mus., Tokyo. Ser. A., 9: 175-210
  2. Kim, E.J. and I.S. Kim. 2007. Population ecology of Kichulchoia brevifasciata (Cobitidae). In: XII European congress of Ichthyology, Cavat, Croatia, Sep. 9-13 2007, 122pp
  3. Kim, I.S. 1997. Illustrated encyclopedia of fauna & flora of Korea. Vol. 37. Freshwater Fishes. The Ministry of Education, Korea, 629pp., (in Korean)
  4. Kim, I.S., E.K. Choi, H. Yang and M.H. Koh. 2003. Karyotype analysis of southern spined loach, Iksookimia hugowolfeldi (Pisces, Cobitidae). Korean J. Ichthyol., 15: 127-129 (in Korean)
  5. Kim, I.S. and J.H. Lee. 1986. A chromosomal study on the genus Cobitis (Pisces: Cobitidae) in the southern part of Korea. Bull. Korean Fish. Soc., 19: 257-264 (in Korean)
  6. Kim, I.S. and W.O. Lee. 1995. Niwaella brevifasciata, a new cobitid fish (Cypriniformes: Cobitidae) with a revised key to the species of Niwaella. Japan. J. Ichthyol., 42: 285-290
  7. Kim, I.S., J.Y. Park and T.T. Nalbant. 1997. Two new genera of loaches (Pisces: Cobitidae: Cobitinae) from Korea. Trav. Mus. Nus. Natl. Hist. Nat (Grigore Antipa), 39: 191-195
  8. Kim, I.S., J.Y. Park and T.T. Nalbant. 1999. The far-east of the genus Cobitis with the description of three new taxa (Pisces: Ostariophysi: Cobitidae). Trav. Mus. Nus. Natl. Hist. Nat. (Grigore Antipa), 41: 373-391
  9. Kim, S.Y., I.S. Kim, K.Y. Jahng and M.H. Chang. 2000. Molecular phylogeny of Korean loaches inferred from mitochondrial DNA cytochrome b sequence. Korean J. Ichthyol., 12: 223-229
  10. Kim, S.Y., J.Y. Park and I.S. Kim. 1999. Chromosome of spined loach, Iksookimia yongdokensis (Pisces: Cobitidae) from Korea. Korean J. Ichthyol., 11: 172-176 (in Korean)
  11. Kimizuka, Y., H. Kobayasi and N. Mizuno. 1982. Geographic distributions and karyotypes of Cobitis takatsuensis and Niwaella delicata (Cobitididae). Japan. J. Ichthyol., 29: 305-310
  12. Lee, H.S. 1988. Karyotype analysis of the genus Cobitis from Korea. Inha University, Ph. D. Thesis, 76pp., (in Korean)
  13. Lee, H.Y., H.S. Lee and C.S. Park. 1986. Karyotype analysis and geographical polymorphism in Korea Cobitis. Kor. J. Genet., 8: 65-74
  14. Levan, A., K. Fredga and A.A. Sandberg. 1964. Nomenclature for centrometric position on chromosomes. Hereditas, 52: 201-220 https://doi.org/10.1111/j.1601-5223.1964.tb01953.x
  15. Miller, P.R. and V. Walter. 1972. A new genus of cyprinodontid fish from Nuevo Leon, Mexico. Contr. Sci. Nat. Hist. Mus. Los Angeles County, 223: 1-13
  16. Nam, M.M., H.J. Yang and B.S. Chae. 1991. The karyotypic analysis of Niwaella multifasciata Wakiya et Mori (Cobitidae), an endemic species in Korea. Korean J. Icthyol., 3: 36-41 (in Korean)
  17. Ohno, S. 1974. Protochordata, Cyclotomata and Pisces. In: John, B.(ed.), Animal cytogenetics Chordata I. Gebruder-Borntra-Ger, Berlin, pp. 1-92
  18. Ojima, Y. and S. Hitotsumachi. 1969. Cytogenetical studies in loaches (Pisces, Cobitidae). Zool. Mag., 78: 138-141
  19. Ojima, Y., M. Hayashi and K. Ueno. 1972. Cytogenetics studies in low vetebrates. Karyotype and DNA studies in 15 species of Japanese Cyprinidae. Japan. J. Genet., 47: 431-440 https://doi.org/10.1266/jjg.47.431