Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

The ABA Effect on the Accumulation of an Invertase Inhibitor Transcript that Is Driven by the CAMV35S Promoter in ARABIDOPSIS

  • Koh, Eun-Ji (College of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Sung June (College of Life Sciences and Biotechnology, Korea University) ;
  • Hong, Suk-Whan (Division of Applied Plant Science, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lee, Hoi Seon (Faculty of Applied Biotechnology and Research Center for Industrial Development of Biofood Materials, College of Agricultural and Life Sciences, Chonbuk National University) ;
  • Lee, Hojoung (College of Life Sciences and Biotechnology, Korea University)
  • Received : 2007.11.20
  • Accepted : 2008.03.18
  • Published : 2008.09.30
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Abstract

Invertase (${\beta}$-D-fructofuranosidase; EC 3.2.1.26) catalyzes the conversion of sucrose into glucose and fructose and is involved in an array of important processes, including phloem unloading, carbon partitioning, the response to pathogens, and the control of cell differentiation and development. Its importance may have caused the invertases to evolve into a multigene family whose members are regulated by a variety of different mechanisms, such as pH, sucrose levels, and inhibitor proteins. Although putative invertase inhibitors in the Arabidopsis genome are easy to locate, few studies have been conducted to elucidate their individual functions in vivo in plant growth and development because of their high redundancy. In this study we assessed the functional role of the putative invertase inhibitors in Arabidopsis by generating transgenic plants harboring a putative invertase inhibitor gene under the control of the CaMV35S promoter. A transgenic plant that expressed high levels of the putative invertase inhibitor transcript when grown under normal conditions was chosen for the current study. To our surprise, the stability of the invertase inhibitor transcripts was shown to be down-regulated by the phytohormone ABA (abscisic acid). It is well established that ABA enhances invertase activity in vivo but the underlying mechanisms are still poorly understood. Our results thus suggest that one way ABA regulates invertase activity is by down-regulating its inhibitor.

Keywords

Acknowledgement

Supported by : Korea Science and Engineering Foundation, Korea University

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