대한약침학회지 (Journal of Pharmacopuncture)

  • 제11권1호
  • /
  • Pages.127-141
  • /
  • 2008
  • /
  • 2093-6966(pISSN)
  • /
  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
권호 표지
DOI QR코드

DOI QR Code

조위승청탕(調胃升淸湯)의 알코올 및 열수(熱水) 추출물이 지방세포 대사에 미치는 영향

Effects of Chowiseungcheng-tang Extracts on the Preadipocytes Proliferation in 3T3-L1 cell line, Lipolysis of Adipocytes in rat, and Localized Fat Accumulation by extraction methods

  • 이재은 (상지대학교 한의과대학 내과학교실) ;
  • 김병우 (상지대학교 한의과대학 내과학교실) ;
  • 임태진 (상지대학교 응용동물과학부) ;
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 권기록 (상지대학교 한의과대학 침구학교실)
  • Lee, Jae-Eun (Department of Internal Medicine, Korean Medical College, Sangji University) ;
  • Kim, Byoung-Woo (Department of Internal Medicine, Korean Medical College, Sangji University) ;
  • Rhim, Tae-Jin (Devision of Animal resourses and life science, Sangji University) ;
  • Kim, Dong-Heui (Department of Environmental Medical Biology, Wonju Medical College, Yonsei University) ;
  • Kwon, Ki-Rok (Department of Acup & Moxi, Korean Medical College, Sangji University)
  • 발행 : 2008.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : The purpose of this study is to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation in 3T3-L1 cell line, lipolysis of adipocytes in rat's epididymal adipocytes and localized fat accumulation of porcine by extraction methods(alcohol and water). Methods : Diminish preadipocytes proliferation and promote lipolysis of adipocytes do primary role to reduce obesity. So, we used 3T3-L1 mouse embryo fibroblasts(preadipocytes) and rat epididymal adipocytes from Sprague-Dawley rats to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation, lipolysis of adipocytes. They were treated with 0.01, 0.1, $1.0mg/m{\ell}$ Chowiseungcheng-tang alcohol and water extracts. And for the purpose of investigating the effects of Chowiseungcheng-tang alcohol and water extracts on the localized fat accumulation, we injected 0.1, 1.0, $10.0mg/m{\ell}$ Chowiseungcheng-tang extracts to porcine fat tissues and observed histological changes of them. Results : Following results were obtained from the preadipocytes proliferation and lipolysis of adipocytes and histological investigation of fat tissues. 1. Chowiseungcheng-tang extracts suppressed preadipocytes proliferation on the high dosage(especially $1.0mg/m{\ell}$), and especially alcohol extracts had better effects. 2. The alcohol extracts of Chowiseungcheng-tang decreased the activity of glycerol-3-phosphate dehydrogenase (GPDH) on the concentrations of 0.1, $1.0mg/m{\ell}$. Alcohol extracts had better effects than water extracts. 3. Chowiseungcheng-tang extracts increased lipolysis of adipocytes on the concentrations of 0.1, $1.0mg/m{\ell}$, and especially on the concentration of $1.0mg/m{\ell}$ alcohol extract of Chowiseungcheng-tang had better effect. 4. The water extract of Chowiseungcheng-tang had significant activity to the destruction of porcine fat cell membranes only on the concentration of $10.0mg/m{\ell}$, but alcohol extracts of Chowiseungcheng-tang had it on all concentrations. Conclusions : The alcohol extracts of Chowiseungcheng-tang had much better effects on the preadipoeytes proliferaton, lipolysis of adipocytes and localized fat accumulation than water extracts.

키워드

참고문헌

  1. 全國韓醫科大學 再活醫學科學敎室. 東醫再活醫學科學. 서울: 書苑堂; 1995, p.570-85
  2. Diehle HS, Darlynple W. Obesity and Digestive disorder and disease. Healthful living. New York: McGraw-Hill; 1973, p.65-8
  3. National Heart, Lung, and Blood Institute in cooperation with The National Institute of Diabetes and Digestive and Kidney Diseases. Clinical Guidelines on the Identification, Evaluation, and Treatment of Overweight and Obesity in Adults. The Evidence Report: National Institute of Health. 1998: Available from URL:http://www.nhlbi.nih.gov/guideline/obesity/ob.home.htm
  4. CDC(Centers for Disease Control and Prevention). Resource Guide for Nutrition and Physical Activity Interventions to Prevent Obesity and Other Chronic Diseases : Available from URL: http://www.cdc.gov/hccdphp/dnpa/sitemap. htm
  5. 탁양주, 이영성, 이진석, 강재헌. 최근 국내 비만 연구의 경향: 1984년부터 2002년까지. 대한비만학회지. 2004;13(1):1-13
  6. 박인상. 東醫四象要訣. 서울: 소나무; 1992, p.156
  7. 李濟馬. 東醫壽世保元. 서울: 행림출판사; 1986, p. 7, 107-11, 123
  8. 서동민, 이상훈, 이재동. 비만 환자에 대한 調胃升淸湯의 효과 및 부작용에 관한 임상적 관찰. 대한침구학회지. 2005;22(3):145-53
  9. 李濟馬. 東醫壽世保元. 서울: 행림출판사; 1986, p. 7, 107-11, 123
  10. 류주열. 東醫四象醫學講座. 경기: 대성의학사; 2003, p.631-8
  11. 보건복지부. 한약재 수급 및 유통관리 규정 제 2조 2항 보건복지부고시 제 2006-69호. 2006
  12. Singh R, Artaza JN, Taylor WE, Braga M, Yuan X, Gonzalez-Cadavid NF, Bhasin S. Testosterone inhibits adipogenic differentiation in 3T3-L1 cells: Nuclear translocation of androgen receptor complex with ${\beta}$-catenin and T-cell factor 4 may bypass canonical Wnt signaling to downregulate adipogenic transcription factors. Endocrinology. 2006;147:141-54 https://doi.org/10.1210/en.2004-1649
  13. Negrel R, Dani C. Culture of adipose precursor cells and cells of clonal lines from animal white adipose tissue. In Adipose Tissue Protocols. G. Ailhaud(ed). Totowa, NJ: Human Press, Inc.; 2001, p 225-37
  14. 안영성, 성낙술, 함인혜, 최호영. 부평의 면역 및 항암 활성 연구. 대한본초학회지. 2004;19(3):117-127
  15. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal. Biochem. 1976;72:248-54 https://doi.org/10.1016/0003-2697(76)90527-3
  16. Rodbell M. Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis. J. Biol. Chem. 1964;239:375-80
  17. Zalatan F, Krause JA, Blask DE. Inhibition of isoproterenol-induced lipolysis in rat inguinal adipocytes in vitro by physiological melatonin via a receptor-mediated mechanism. Endocrinology. 2001;142:3783-90 https://doi.org/10.1210/en.142.9.3783
  18. McGowan MW, Artiss JD, Strandbergh DR, Zak B. A peroxidase-coupled method for the colorimetric determination of serum triglycerides. Clin. Chem. 1983;29:538-42
  19. Steel RGD, Torrie JH. Principles and Procedures of Statistics, 2nd ed. New York: McGraw-Hill; 1980, p. 187-8
  20. 이광우. 비만증의 진단과 평가. 대한비만학회지. 1992;1(1):1-4
  21. 대한비만학회. 임상 비만학(제2판). 서울: 고려의학; 2001, p.19-28
  22. 김영설. 비만증의 진단과 평가. 대한비만학회지 제 11권 부록 제 2호(대한비만학회 제 5회 연수강좌). 2002:3-8
  23. Fontbonne A, Thibult N, Eschwge E, Ducimetire p.Body fat distribution and coronary heart disease mortality in subjects with impaired glucose tolerance or diabetes mellitus: the Paris Prospective Study, 15-year follow up. Diabetologia. 1992;35:464-8 https://doi.org/10.1007/BF02342445
  24. Folsom AR, Kaye SA, Sellers TA, Hong C-P, Cerhan JR, Potter JD, Prineas RJ. Body fat distribution and 5-year risk of death in older women. Journal of the Amerian Medical Association. 1993;269:483-7 https://doi.org/10.1001/jama.269.4.483
  25. Kalmijn S, Curb JD, Rodriguez BL, Yano K, Abbott RD. The association of body weight and anthropometry with mortality in elderly men: The Honolulu Heart Program International Journal of Obesity. 1999;23:395-402 https://doi.org/10.1038/sj.ijo.0800832
  26. Reaven GM. Role of insulin resistance in human disease. Diabetes. 1988;37:1595-607 https://doi.org/10.2337/diabetes.37.12.1595
  27. Haffner SM, Valdez RA, Hazuda HP, Mitchell BD, Morales PA, Stern Mp.Prospective analysis of the insulin resistance syndrome (Syndrome X). Diabetes. 1992;41:715-22 https://doi.org/10.2337/diabetes.41.6.715
  28. Grundy SM. Hypertriglyceridemia, insulin resistance, and the metabolic syndrome. Am J Cardiol. 1999;83:25F-9F https://doi.org/10.1016/S0002-9149(99)00211-8
  29. Kissebah AH, Krakower GR. Regional adiposity and morbidity. Physiological Reviews. 1994;74:761-811
  30. 王冰. 黃帝內經靈樞. 臺北: 臺灣中華書局; 1972, p. 155, 209
  31. 李梴. 編註醫學入門. 서울: 大星文化社; 1990, p.(外集卷一)278, p.(外集卷二)108
  32. 陳貴延. 實用中西醫結合診斷治療學. 北京:中國醫藥科學技術出版社; 1991, p.682-9
  33. 김달래, 백태현. 四象體質과 肥滿의 상관성에 관한 임상적 연구. 사상의학회지. 1996;8(1):319-35
  34. 조홍건, 김병덕. 肥滿症의 原因과 病機및 治法에 관한 文獻的考察. 대전대학교 한의학연구소 논문집. 1992;1(2):61-71
  35. 신현대, 김성수, 이응세. 肥滿의 治療에 關한 臨床的 比較硏究. 대한한의학회지. 1992;13(2):63-73
  36. 박상용, 이병열. 肥滿治療에 대한 鍼灸 및 藥物治療의 臨床的硏究. 大田大學校 論文集 韓醫學編. 1994;2(2):163-85
  37. 정선희, 남상수, 김용석, 이재동, 최도영, 고형균 등. 肥滿患者의 電鍼治療 臨床例. 대한침구학회지. 1999;16(3):39-56
  38. 김연섭, 변성희, 서부일, 김상찬, 국 만, 조응행. 비만 치료 및 예방에 대한 한약의 효능 연구(II). 대한본초학회지. 2000;15(1):73-82
  39. 윤대환, 강자돈, 주준성, 채우석, 나창수. 高脂肪食餌로 誘發된 肥滿白鼠모델에서 蒼朮, 薏苡仁, 麻黃, 蒼朮合薏苡仁, 麻黃合綠茶 藥鍼이 血淸脂質 및 肝機能에 미치는 影響. 제 3회 대한약침학회 학술대회논문집. 2004;7(1):87-100
  40. 지준환, 이준무. 赤小豆蒸溜藥鍼이 肥滿쥐의 脂質構成, 肝臟機能, 抗酸化效果 및 分子生物學的 特性에 미치는 影響. 대한약침학회지. 2005;8(2):67-82 https://doi.org/10.3831/KPI.2005.8.2.067
  41. 조희철, 이시형, 신조영, 김강산, 조남근, 권기록, 임태진. 熊膽과 牛黃藥鍼이 지방세포 대사에 미치는 영향. 대한침구학회지. 2007;24(4) :125-42
  42. 정영표, 윤여충, 윤대환. 胃兪의 山査藥鍼이 高脂肪食餌로 誘發된 肥滿白鼠에 미치는 影響. 대한침구학회지. 2007;24(4):55-68
  43. 강수우, 위통순, 윤대환. 脾兪에 대한 羅蔔子藥鍼이 고지방 식이로 유발된 肥滿白鼠에 미치는 영향. 대한침구학회지. 2007;24(5):113-25
  44. 주집성, 채우석, 신미숙, 나창수. 肥滿治療를 위한 經穴 및 藥物에 관한 考察. 대한경락경혈학회지. 2003;20(3):147-62
  45. 김호철. 韓藥藥理學. 서울: 集文堂; 2001, p. 63-6
  46. Astrup A, Breum L, Toubro S, Hein P, Quaade F. Ephedrine and weight loss. Int J Obes Relat Metab Disord. 1992 Sep;16(9):715
  47. Green H, Kehinde O. Sublines of mouse 3T3 cells that accumulate lipid. Cell. 1974;1:113-6 https://doi.org/10.1016/0092-8674(74)90126-3
  48. Green H, Kehinde O. An established preadipose cell line and its differentiation in culture. Cell. 1974;3:127-33 https://doi.org/10.1016/0092-8674(74)90116-0
  49. Green H, Kehinde O. An established preadipose cell line and its differentiation in culture II. Factors affecting the adipose conversion. Cell. 1975;5:19-27 https://doi.org/10.1016/0092-8674(75)90087-2
  50. Green H, Kehinde O. Spontaneous heritable changes leading to increased adipose conversion. Cell. 1976;7:105-13 https://doi.org/10.1016/0092-8674(76)90260-9

피인용 문헌

  1. CST, an Herbal Formula, Exerts Anti-Obesity Effects through Brain-Gut-Adipose Tissue Axis Modulation in High-Fat Diet Fed Mice vol.21, pp.11, 2016, https://doi.org/10.3390/molecules21111522
  2. The Clinical Study about Qualitative and Quantitative Characteristics of Acupuncture Sensation According to the Type of Pharmacopuncture: Study about BUM Pharmacopuncture, Mountain Ginseng Pharmacopuncture and Sciatica No. 5 Pharmacopuncture vol.30, pp.5, 2013, https://doi.org/10.13045/acupunct.2013043