Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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A study on the comparison of antioxidant effects between hot pepper extract and capsaicin

고추와 Capsaicin의 항산화 효능 비교

  • 강현민 (상지대학교 한의과대학 침구학교실) ;
  • 박희수 (상지대학교 한의과대학 침구학교실) ;
  • 임태진 (상지대학교 응용동물과학부) ;
  • 권기록 (상지대학교 한의과대학 침구학교실)
  • Published : 2008.03.31
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Abstract

Objective : The objective of this study was to compare the antioxidant effects of hot pepper extract and capsaicin. Methods : In vitro, antioxidant activities were examined by DPPH radical scavenging activity, total antioxidant capacity(TAC), oxygen radical scavenging capacity(ORAC), inhibition of induced lipid peroxidation using liver mitochonria and total phenolic contents. Results : 1. DPPH free radical scavenging activities at the concentrations of both 1 and $10mg/m{\ell}$ were 1.2 to 1.9 times higher in capsaicin than in hot pepper extract. The concentration of capsaicin required for 50% radical scavenging was lower than that of hot pepper extract(3.9 vs $5.9mg/m{\ell}$), indicating that capsaicin had higher DPPH radical scavenging activity than hot pepper extract. 2. Total antioxidant capacities of capsaicin at the concentrations of 0.1 and 1mg/ml(13.8 and 41.3 nmol Trolox equivalent) were not significantly different from those at the concentrations of 1 and $10mg/m{\ell}$(11.4 and 41.2nmol Trolox equivalent), indicating that capsaicin showed 10 times higher ABTS radical scavenging activity compared to hot pepper extract. 3. ORAC of capsaicin at the concentrations of 1, 5, 10 and 100 mg/ml were 0.04, 0.17, 0.29 and 1.74nmol gallic acid equivalent, respectively. On the other hand, ORAC of hot pepper extract at the concentrations of 1, 5, 10 and $100{\mu}g/m{\ell}$ were 0.15, 0.44, 0.75 and 2.49nmol gallic acid equivalent, respectively, indicating that capsaicin showed higher peroxyl radical scavenging activity than hot pepper extract. 4. Inhibition of lipid peroxidation caused by hot pepper extract at the concentrations of 1 and $10mg/m{\ell}$ were 12.2 and 61.4%, respectively. Inhibition of lipid peroxidation caused by capsaicin at the concentrations of 1 and $10mg/m{\ell}l$ were 64.0 and 96.8%, respectively. Thus capsaicin showed 10 times stronger effect in inhibiton of lipid peroxidation than hot pepper extract. 5. Total phenolic contents of hot pepper extract at the concentrations of 0.1 and $1mg/m{\ell}$ were 1.4 and 20.8nmol gallic acid equivalent, respectively. Total phenolic contents of capsaicin at the concentrations of 0.1 and $1mg/m{\ell}$ were 6.1 and 55.4 nmol gallic acid equivalent, respectively, indicating that capsaicin had 2.7 to 4.3 times higher total phenolic contents than hot pepper extract. Conclusions : In summary, the results of this study demonstrate significant antioxidant activity of hot pepper extract, although the activity was lowered compared to capsaicin, suggesting that hot pepper extract play a role in prevention of oxidative-related diseases.

Keywords

References

  1. 농촌진흥청 농업생명공학연구원. 유전자원 특성조사 및 관리요령 RED PEPPER 고추, 2004; 3
  2. 중약대사전편찬위원회. 완역중약대사전 권2, 정담, 1997; 668-670
  3. 이충영, 우상규, 이운수, 권익부. 고속 액체크로마토 그래피에 의한 고추중의 신미성분분석. 한국식품위생안전성학회지. 1989; 4(3): 191-198
  4. 유리나. 김치내 고추가루 매운성분(켑사이신)의 면역증강 및 항성인병성 효과. 김치의 과학과 기술 권1, 1995; 143-144
  5. 손정일, 고추가루를 이용한 기능성 소화불량의 치료. 대한소화관운동학회지. 2002; 8(2): 208-209
  6. 임기원, 서혜정, 이수천. 운동선수에 있어서 고추가루 섭취가 안정시 에너지대사에 미치는 영향. 운동영양학회지. 1997; 1(1): 61-67
  7. 정영옥, 유태종. 고추에서 분리한 미생물의 발육과 capsaicin의 분해에 관한 연구. 한국영양학회지. 1976; 9(3): 18-25
  8. Chin-Lin Hsu, Gow-Chin Yen. Effect of capsaicin on induction pf apoptosis and inhibition of adipogenesis in 3T3-L1 cells. J. Agric. Food Chem. 2007; 55: 1730-1736 https://doi.org/10.1021/jf062912b
  9. 이치호, 최병규, 김천제, 최도영, 정구용, 임성천. 베이컨 육에 있어서 고추 Capsaicin 및 Oleoresin의 항산화 작용에 관한 연구. 한국식품과학회지. 1994; 26(5): 496-499
  10. 박정순, 이진옥, 유리나. 고추의 매운맛 성분 Capsaicin의 항산화 활성. 식품산업과 영양. 1997; 2(2): 93
  11. 이용수, 강영신, 이지선, 세브달리나, 니콜로바, 김정애. HepG2 사람 간암세포에서 캅사이신에 의한 아포토시스에 있어서 NADPH 옥시다제-매개로 생성된 활성산소종의 관련성. 영남대학교 약품개발연구소 연구업적집. 2004; 14(0): 146-153
  12. 장종선, 이지선, 이지열, 김정애. 캅사이신에 의한 MBT-2 방광암세포에서의 활성산소종 생성의 감소와 아포토시스 유도에 관한 연구. 영남대학교 약품개발연구소 연구업적집. 2005; 15(0): 37-43
  13. Hovius, R., H. Lambrechts, K. Nocolay and B. de Kruijff. Improved methods to isolate and subfractionate rat liver mitochondria. Lipid composition of the inner and outer membrane. Biochim. Biophys. Acta. 1990; 1021: 217-226 https://doi.org/10.1016/0005-2736(90)90036-N
  14. Malterud, K.E., T.L. Farbrot, A.E. Huse and R.B. Sund. Antioxidant and radical scavenging effects of anthraquinones and anthrones. Pharmacology 1993; 47: 77-85 https://doi.org/10.1159/000139846
  15. Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 1999; 26: 1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  16. Erel, O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem. 1999.; 37: 277-285 https://doi.org/10.1016/j.clinbiochem.2003.11.015
  17. Huang, D., B. Ou, M. Hampsch-Woodill, J. A. Flanagan and R. L. Prior. High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J. Agric. Food Chem. 1999; 50: 4437-4444 https://doi.org/10.1021/jf0201529
  18. Stacey, N.H. and C.D. Klaassen. Inhibition of lipid peroxidation without prevention of cellular injury in isolated rat hepatocytes. Toxicol. Appl. Pharm. 1999; 58: 8-18 https://doi.org/10.1016/0041-008X(81)90110-1
  19. Singleton, V.L. and R. Orthofer. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999; 299: 152-178 https://doi.org/10.1016/S0076-6879(99)99017-1
  20. Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall. Protein measurements with the Folin phenol reagent. J. Biol. Chem. 1951; 193: 265-275
  21. Steel, R.G.D. and J.H. Torrie. Principles and procedures of statistics, 2nd ed, McGraw-Hill, New York, 1999; 186-187
  22. 정천순, 유근창, 김종화, 김학기. HPLC에 의한 고추의 capsaicin 측정. 강원대학교 농업과학연구소논문집. 1995; 6(1): 9-14
  23. Surh, Y. J. Anti-tumor promotiong potential of selected spice ingredients with antioxidative and-inflammatory activities: a short review. Food Chem. Taxicol. 2002; 40: 1091-1097 https://doi.org/10.1016/S0278-6915(02)00037-6
  24. Yoshioka, M.; St-Pierre, S; Drapeau, V.; Dionne, I.; Doucet, E.; Suzuki, M..; Tremblay, A. Effects of red pepper on appetite and energy intake. Br. J. Nutr. 1999; 82: 115-123
  25. Kawada, T.; Hagihara, K. I.; Iwai, K. Effects of capsaicin on lipid metabolism in rats fed a high fat diet. J. Nutr. 1986(a); 116: 1272-1278
  26. Morre, D. J.; Cheuh, P. J.; Morre, D. M. Capsaicin inhibits preferentially the NADH oxidase and growth of transformed cells on culture. Proc. Natl. Acad. Sci. U.S.A 1995; 92: 1831-1835 https://doi.org/10.1073/pnas.92.6.1831
  27. Macho, A.; Sancho, R.; Minassi, A.; Appendino, G.; Lawen, A.; Munoz, E. Involvement of reactive oxygen species in capsaicinoid-induced apoptosis in transformed cells. Free Radical Res. 2003; 37: 611-619 https://doi.org/10.1080/1071576031000083215
  28. Devy, C. and Gautier, R. New perspectives on the biochemistry of superoxide anion and the efficiency of superoxide dismutase. Biochem. Pharmacol. 1990; 39: 399-405 https://doi.org/10.1016/0006-2952(90)90043-K
  29. Kuramoto, T. Development and application of food materials from plant extracts such as SOD. Up to date Food Processing. 1992; 27: 22-23
  30. Fridovich, I. Biological effects of the superoxide radical. Adv. Enzymol. 1986; 58: 62
  31. Yoshizawa, S., Horiuchi, T., Yoshida, T., Okuda,T.Antitumor promoting activity of (-)- epigallocatechin gallate, the main constitutent of tannin in green tea. Phytother. Res. 1987; 1: 44-47 https://doi.org/10.1002/ptr.2650010110

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