Journal of Crop Science and Biotechnology
- 제11권4호
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- Pages.237-242
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- 2008
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- 1975-9479(pISSN)
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- 2005-8276(eISSN)
Isolation and Characterization of a Ds-tagged liguleless Mutant in Rice (Oryza sativa. L)
- Ahn, Byung-Ohg (National Academy of Agricultural Science, RDA) ;
- Ji, Sang-Hye (National Academy of Agricultural Science, RDA) ;
- Yun, Doh-Won (National Academy of Agricultural Science, RDA) ;
- Ji, Hyeon-So (National Academy of Agricultural Science, RDA) ;
- Park, Yong-Hwan (National Academy of Agricultural Science, RDA) ;
- Park, Sung-Han (National Academy of Agricultural Science, RDA) ;
- Lee, Gi-Hwan (National Institute of Crop Science, RDA) ;
- Suh, Seok-Cheol (National Academy of Agricultural Science, RDA) ;
- Lee, Myung-Chul (National Academy of Agricultural Science, RDA)
- 발행 : 2008.12.31
초록
A liguleless mutant, which showed complete loss of lamina joint region at the junction between leaf blade and leaf sheath, was isolated from a Ds insertional mutants derived from the source cultivar, Dongjin. This mutant could not affect other developmental patterns like phyllotaxis. Southern blot analysis, using GUS as a probe, revealed that the liguleless mutant contained three Ds copies transposed in the rice genome. Among the four genomic sequences flanking the Ds, one was mapped in the intergenic region (31661640 - 31661759), and the other two predicted a protein kinase domain (12098980 - 12098667) as an original insertion site within a starter line used for massive production of Ds insertional mutant lines. Another predicted and inserted in first exon of liguleless 1 protein (OsLG1) that was mapped in coding region (LOC_Os04g56170) of chromosome 4. RT-PCR revealed that the OsLG1 gene was not expressed liguleless mutants. Structure analysis of OsLG1 protein revealed that it predicted transcription factor with a highly conserved SBP domain consisting of 79 amino acids that overlapped a nuclear localization signal (NLS). RT-PCR revealed that OsLG1 is mainly expressed in vegetative organs.