Journal of Crop Science and Biotechnology
- 제11권4호
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- Pages.227-232
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- 2008
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- 1975-9479(pISSN)
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- 2005-8276(eISSN)
Proteomic Analysis of Drought Stress-Responsive Proteins in Rice Endosperm Affecting Grain Quality
- Mushtaq, Roohi (International Rice Research Institute) ;
- Katiyar, Sanjay (Indira Gandhi Agricultural University) ;
- Bennett, John (International Rice Research Institute)
- 발행 : 2008.12.31
초록
Drought stress is one of the major abiotic stresses in agriculture worldwide. We report here a proteomic approach to investigate the impact of post-fertilization drought on grain quality in rice seed endosperm (Oryza sativa cv. IR-64). Plants were stressed for 4 days at 3 days before heading. Total proteins of endosperm were extracted and separated by two-dimensional gel electrophoresis. Not many protein spots showed differential accumulation in drought-stressed samples. More than 400 protein spots were reproducibly detected, including three that were up-regulated and five down-regulated. Mass spectrometry analysis and database searching helped us to identify six spots representing different proteins. Functionally, the identified proteins were related to protein synthesis and carbohydrate metabolism, such as Granule-Bound Starch Synthase (GBSS, Wx protein), which is thought to play a very important role in starch biosynthesis and quality, a very crucial factor in determining rice grain quality.