DOI QR코드

DOI QR Code

마우스에서 vitamin E의 장.단기적 항스트레스 효과

Anti-stress Effect during Long and Short-Term of Vitamin E in Mice

  • 이승진 (건국대학교 대학원 생물공학과) ;
  • 고민석 (건국대학교 대학원 생물공학과) ;
  • 강상모 (건국대학교 대학원 생물공학과)
  • Lee, Seung-Jin (Department of Bioengineering at the Postgraduate School, Konkuk University) ;
  • Go, Min-Seok (Department of Bioengineering at the Postgraduate School, Konkuk University) ;
  • Kang, Sang-Mo (Department of Bioengineering at the Postgraduate School, Konkuk University)
  • 발행 : 2008.12.31

초록

본 연구에서는 일정기간 스트레스를 받을 때 vitamin E 투여가 스트레스를 얼마나 장 단기적으로 방어할 수 있는지 알아보았다. ICR 마우스를 사람의 나이 20대에 해당되는 5개월까지 사육한 후, 8주간의 스트레스를 가하고 사람의 나이 노년기에 해당되는 18개월 될 때까지 사육하여 스트레스로 인한 생체 내의 산화적 손상지표로 혈청과 간의 대사산물의 변화를 조사하였다. 그 결과, 스트레스는 장 단기적으로 모두 혈청의TG, AST 함량을 증가시키고, HDL-cholesterol 함량을 저하시키는 것에 반해, 여기에 vitamin E의 투여는 스트레스를 받은 쥐에서 혈청 TG와 AST의 함량을 감소시키고, HDL-cholesterol의 함량을 증가시키는 효과를 보여주었다. 스트레스는 간조직의 TAS, SOD 함량 감소와 MDA 함량 증가를 야기시켰으며, 스트레스 받은 쥐에 vitamin E의 투여는 TAS, SOD의 함량을 증진시키고, MDA의 함량을 저하시키는데 긍정적 영향을 주었다. 따라서 젊었을 때 한때 받은 스트레스는 노년기의 TG, HDLcholesterol, AST, TAS, SOD 그리고 MDA의 수치 모두에 있어서 부정적인 영향을 미치는 것으로 나타났다. 이러한 결과를 통해서 한때 어느 기간 받은 스트레스는 시간이 지난 노년기까지 혈청과 간조직에 부정적 영향을 주며 vitamin E는 이를 어느 정도 보호하는데 긍정적 인 효과를 나타낸다는 것을 알 수 있었다.

This study was to evaluate the protective effect of vitamin E against long and short-term stress in ICR mice. Two groups which had been bred for 5 months (equivalent to human beings aged 20) were treated by immobilization stress for 8 weeks with or without vitamin E, and one out of two groups was continuously bred until they become 18 months old (equivalent to human senescence) with or without Vitamin E. Afterwards, the changes of serum and hepatic metabolites were investigated on the basis of the index of stress-related in vivo oxidative damage. As a result, it was found that stress increases serum triacylglycerol and aspartate aminotransferase (AST) in the long and short-term, and decreases serum HDL-cholesterol. In addition, stress concerned the decrease of total antioxidant status (TAS) and superoxide dismutase (SOD) as well as the increase of malondialdehyde (MDA) in liver. These results suggest that stress in one’s youth causes negative results in TG, HDL-cholesterol, AST, TAS, SOD and MDA measured in one’s senescent. The administration of vitamin E in the stressed mice decreases serum TG and AST that are increased by stress, and exerts influence on the increase of serum HDL-cholesterol. Also vitamin E recovered the values of liver TAS, SOD and MDA in the stressed mice. In conclusion, vitamin E represented protective effect in the stressed mice to some degree.

키워드

참고문헌

  1. Ghrousos, G. P. (2000) The role of stress and the hypothalamicpituitary-adrenal axis in the pathogenesis in the metabolic syndrome: Neuroendocrine and target tissue-related causes. Int. J. Obesity. 24, S50-S55 https://doi.org/10.1038/sj/ijo/0801278
  2. Elliot, G. R. and Eisdorfer, C. (1982) Stress and human health. New York, Springer, 2, 1-20
  3. Thomson, C. B. (1995) Apoptosis in the pathogenesis and treatment of disease. Science. 267, 1456-1462 https://doi.org/10.1126/science.7878464
  4. Gutteridge, J. N. C. (1995) Lipid peroxidation and antioxidants as biomarker of tissue damages. Clin. Chem. 41, 1819-1828
  5. Chow, C. K. (1991) Vitamin E and oxidative stress. Free Radical Bio. Med. 11, 215-232 https://doi.org/10.1016/0891-5849(91)90174-2
  6. Tiidus, P. M. and Houston, M. E. (1995) Vitamin E status and response to exercise training. Sports Med. 20, 12-23 https://doi.org/10.2165/00007256-199520010-00002
  7. Kagan, V. E., Spirichev, V. B. and Serbinova, E. A. (1994) The significance of vitamin E and free radicals in physical exercise. In Nutrition in exercise and sport, Wolinsky, I. (2nd ed.) pp. 185-213, CRC Press, Boca Raton
  8. Avellini, L., Chiaradia, E. and Gaiti, A. (1999) Effect of exercise training, selenium and vitamin E on some free radical sacvengers in horse. Comp. Biochem. Phys. B. 123, 147-154 https://doi.org/10.1016/S0305-0491(99)00045-0
  9. Livne, E., Laufer, D. and Blumenfeld, I. (1997) Comparison of in vitro response to growth hormone by chondrocytes from mandibular condyle cartilage of young and old mice. Calcified. Tissue. Int. 7, 61-62
  10. Park, S. Y. (2001) Study on the oxidative damage induced by stress and its recovery in mice. Department of Microbial Engineering. Dissertation of Ph. D. Konkuk Universtiy. Seoul
  11. Kissebah, A. H. (1974) Stress hormines and lipid metabolism. Proc. R. Soc. Med. 67, 665-667
  12. Howard, N. H., Dieter, M. K., Poetro, A., Gabriele, B. B., Giuseppe, G., Juliana, H., Hazel, P. and Alex, S. (1994) Biochemical and cytotoxic characterstics of an in vivo circulating oxidized LDL. J. Lipid Res. 35, 669-677
  13. Collett, C., Pares-Herbute, N., Monnier, L. H. and Cartry, E. (1988) Platelet function in type I diabetes: Effects of supplementation with large doses of vitamin E. Am. J. Clin. Nutr. 47, 256-261 https://doi.org/10.1093/ajcn/47.2.256
  14. Gittleman, B., Shatin, L., Bierenbaum, M. L., Fleisohman, A. I. and Hqyton, T. (1968) Effects of quantified stressful stimuli on blood lipids in man. J. Nutr. Ment. Dis. 147, 196-201 https://doi.org/10.1097/00005053-196808000-00011
  15. Lehtonen, A. J. (1978) The effect of vigorous physical activity at work on serum lipids with a special reference to serum highdensity lipoprotein cholesterol. Acta. Physiol. Scand. 104, 117-121 https://doi.org/10.1111/j.1748-1716.1978.tb06257.x
  16. Kruit, J. K., Groen, A. K., van Berkel, T. J. and Kuipers, F. (2006) Emerging roles of the intestine in control of cholesterol metabolism. World. J. Gastroenterol. 12, 6429-6439 https://doi.org/10.3748/wjg.v12.i40.6429
  17. Lim, S. S., Kim, M. H. and Lee, J. H. (1997) Effect of Artemisia princeps var orientalis and Circium japonicum var ussuriense on liver function body lipid and bile acid of hyperlipidemic rat. Korean J. Nutr. Soc.. 30, 797-802
  18. Woo, Y. S. (1992) Studies on the effects of slaughter stress on the blood picture and serum chemical values of pigs. Dissertation of Ph. D. Konkuk Universtiy. Seoul
  19. Hernry, J. B. (2007) Measurement of lipids and evaluation of lipid disorders. In Clinical diagnosis and management by Laboratory Methods, Levy, M. D. pp. 171-198, Elsevier Science Health Science div, Churchill
  20. Rao, M. S., Pareddy, K., Abecassis, M. and Hashimoto, T. (2001) Regeneration of liver with marked fatty change following partial hepatectomy in rats. Digest. Dis. Sci. 46, 1821-1826 https://doi.org/10.1023/A:1010654908938
  21. Cherubini, A., Zuliani, G. and Costantini, F. (2001) High vitamin E plasma levels and low low-density lipoprotein oxidation are associated with the absence of atheros-clerosis in octogenarians. J. Am. Geriatr. Soc. 49, 651-654 https://doi.org/10.1046/j.1532-5415.2001.49128.x
  22. Gale, C. R., Ashurst, H. E., Powers, H. J. and Martyn, C. N. (2001) Antioxidant vitamin status and carotid atherosclerosis in the elderly. Am. J. Clin. Nutr. 74, 402-408 https://doi.org/10.1093/ajcn/74.3.402
  23. Lovin, R., Cottle, W., Pyke, I., Davanagh, M. and Belcastro, A. N. (1987) Are induces of free radical damage related to exercise intensity. Eur. J. Appl. Physiol. 6, 313-316
  24. Lawler, J. M., Powers, S. K., Dijk, H. V., Visser, T., Kordus, M. J. and Ji, L. L. (1994) Metabolic and antioxidant enzyme activities in the diaphragm: effects of acute exercise. Res. Physiol. Neurobiol. 96, 139-149
  25. Ji, L. L. (1993) Blood glutathione status during exercise; Effect of carbohydrate supplementation. J. Appl. Physiol. 74, 788-792. 26 https://doi.org/10.1152/jappl.1993.74.2.788
  26. Halliwell, B. and Gutteridge, J. M. C. (1989) In Free radicals in biology and medicine, (2nd ed.) Clarendon Press, Oxford
  27. Yun Mei, K. R., Gawai., Zhongzhen, Nie., Vickmar. and Robert, H. Helfert. (1999) Age related reductions in the activities of antioxidant enzymes in the rat inferior colliculus. Hearing. Res. 135, 169-180 https://doi.org/10.1016/S0378-5955(99)00103-3
  28. Scarpa, M., Rigo, A., Maiorino, M., Ursini, F. and Gregolin, C. (1984) Formation of $\alpha$-tocopherol radical and recycling of $\alpha$- tocopherol by ascorbate during peroxidation of phophatidylcholin liposomes. An electron paramagnetic resorbance study. Biochem. Biophys. Res. Comm. 801, 215-219
  29. Okimoto, K., Hamazaki, K., Lwagaki, H., Orita, K. and Mori, A. (1995) Effect of picibanil on the scavenging effect of free radicals produced during liver regeneration in the rat. Acta. Med. Okayama. 49, 75-79
  30. Bedossa, P., Houglum, K., Trautwein, C., Holstege, A. and Chojkier, M. (1994) Stimulation of collagen $\alpha$ I(I) gene espression is associated with lipid peroxidation in hepatocellular injury: a Link tot issue fibrosis. Hepatology. 19, 1262-1271
  31. Nakatsukasa, H., Evarts, R. P., Hsia, C. C. and Thorgeirsson, S. S. (1990) Transforming growth factor-$\beta^1$1 and type I procollagen transcripts during regeneration and early fibrosis of rat liver. Lab Invest. 63, 171-180
  32. Armendariz-Borunda, J., Seyer, J. M., Kang, A. H. and Raghow, R. (1990) Regulation of TGF-$\beta$ gene espression in rat liver intoxicated with carbon tetrachloride. FASEB J. 4, 215-221 https://doi.org/10.1096/fasebj.4.2.2298342
  33. Brenner, D. A., Veloz, L., Jaenisch, R. and Alcorn, J. M. (1993) Stimulation of the collagen $\alpha$ I (I) endogenous gene and transgene in carbon tetrachloride-induced hepatic fibrosis. Hepatology. 17, 287-292
  34. Castilla, A., Prieto, J. and Fausto, N. (1991) Transforming growth factor $\beta$1 and $\alpha$ in chronic liver disease. N. Eng. J. Med. 324, 933-940 https://doi.org/10.1056/NEJM199104043241401
  35. Traber, M. G. (2001) Does vitamin E decrease heart attack risk summary and implications with respect to dietary recommendations. J. Nutr. 131, 395-397 https://doi.org/10.1093/jn/131.2.395S

피인용 문헌

  1. Long- and short-term effects of vitamin E administration along with stress on skin tissues of mice vol.55, pp.1, 2012, https://doi.org/10.1007/s13765-012-0018-z