Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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A Simple Purification Method and Chemical Properties of Potphyran from Porphyra yezoensis

Porphyran의 신속한 정제 방법 및 화학적 특성

  • Park, Jin-Hee (Institute of biomedical Science, Hanyang University) ;
  • Koo, Jae-Geun (Department of food science and biotechnology, Kunsan National University)
  • 박진희 (한양대학교 의생명연구원) ;
  • 구재근 (군산대학교 식품생명공학과)
  • Published : 2008.12.31
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Abstract

A simple method for the purification of porphyran from laver Porphyra yezoensis was developed to obtain information for the development of food materials with biological functionality. Crude porphyran (CP) was extracted from dried laver in boiling water for 3 h, and then fractionated using cetylpyridinium chloride into an acidic fraction (CP-F1) and a neutral fraction (CP-F2). CP-F1 was fractionated further by fractional ethanol precipitation. Fraction CP-F1-70, precipitated at an ethanol concentration of 61-70% was the major fraction containing 68.1% of the yield from the initial fraction CP-F1. The CP-F1-70 fraction displayed a single band on Sepharose CL-4B with a molecular mass of 550 kDa, indicating a homogeneous polysaccharide. The molar ratio of galactose, 3,6-anhydro-L-galactose, 6-0-methyl-D-galactose and ester sulfate of CP-F1-70 was 1:0.32:0.07:0.53. This method is very useful for rapid and large-scale preparation of purified porphyran because it is compatible with mass production.

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References

  1. Anderson, N.S., T.C.S. Dolan and D.A. Rees. 1965. Evidence for a common structural pattern in the poly- saccharide sulphates of the Rhodophyceae. Nature, 13, 1060-1062
  2. Anderson, N.S. and D.A. Rees. 1965. Porphyran: A polysaccharide with a masked repeating structure. J. Chem. Soc., 5880-5887 https://doi.org/10.1039/jr9650005880
  3. Brasch, D.J., H.M. Chang, C.T. Chuah and L.D. Melton. 1981. The galactan sulfate from the edible red alga Porphyra columbina. Carbohydrate Res., 97, 113- 125 https://doi.org/10.1016/S0008-6215(00)80529-7
  4. Dodgson, K.S. and R.G. Price. 1962. A note on the determination of the ester sulfate from the sulphated polysaccharides. Biochem. J., 84, 106-110 https://doi.org/10.1042/bj0840106
  5. Dubois, M., K.A. Gills, J.K. Hamilton, P.A. Rebers and F. Smith. 1956. Colorimetric method for the deter- mination of sugars and related substances. Anal. Biochem., 28, 350-356
  6. Furneux, R.H., I.J. Miller and T.T. Stevenson. 1990. Agaroids from New-zealand members of the Graci- lariaceae (Gracilariales, Rhodophyta) a novel di- methylated agar. Hydrobiologia, 204/205, 645-654 https://doi.org/10.1007/BF00040300
  7. Gretz, M.R., E.L. McCandless, J.M. Aronson and M.R. Sommerfeld. 1983. The galactan sulphates of the conchocelis phases of Porphyra leucostricta and Bangia atropurpurea (Rhodophyta). J. Exp. Bot., 34, 705-711 https://doi.org/10.1093/jxb/34.6.705
  8. Jung, K.J., B.M. Jung and S.B. Kim. 2001. Effect of porphyran isolated from laver, Porphyra yezoensis, lipid metabolism in hyperlipidemic and hyper- cholesterolemic rats. J. Food Sci. Technol., 33, 633- 640
  9. Kwon, M.J. and T.J. Nam. 2006. Porphyran induces apoptosis related signal pathway in AGS gastric cancer cell lines. Life Science, 79, 1956-1962 https://doi.org/10.1016/j.lfs.2006.06.031
  10. Lowry, O.H., N.J. Rosebrough, L. Farr and R.J. Rindall. 1951. Protein measurement with the folin phenol reagent. J. Bio. Chem., 193, 265-275
  11. Morrice, L.M., M.W. McLean, W.F. Long and F.B. Williamson. 1983. Porphyran primary structure. Eur. J. Biochem., 133, 673-684 https://doi.org/10.1111/j.1432-1033.1983.tb07516.x
  12. Noda, H., H. Amano and K. Aeashima. 1989a. Studies on the anti-tumour activity of marine algae. Nippon Suisan Gakkaishi, 55, 1259-1264
  13. Noda, H., H. Amano and K. Aeashima. 1989b. Antitumour activity of polysaccharides and lipids from algae. Nippon Suisan Gakkaishi, 55, 1265-1271 https://doi.org/10.2331/suisan.55.1265
  14. Park, J.H., J.G. Koo, J.R. Do, C.B. Yang and S.K. Woo. 1997. Effect of extraction temperature and pH on the chemical properties of crude porphyran extracted from Porphyra yezoensis. J. Korean Fish. Soc., 30. 127-131
  15. Peat, S., J.R. Turvey and D.A. Rees. 1961. Carbohydrates of the red alga, Porphyra umbilicalis. J. Chem. Soc., 1590-1595 https://doi.org/10.1039/jr9610001590
  16. ees, D.A. and E. Conway. 1962. The structure and biosynthesis of porphyran: A comparison of some samples. Biochem. J., 84, 411-416 https://doi.org/10.1042/bj0840411
  17. Scott, J.E. 1965. Fractionation by precipitation with quaternary ammonium salts. In: Methods in carbo- hydrate chemistry, Vol. 5, R.L Whistler et al., ed. Academic Press, New York, 38-40
  18. Teruya, T.T., T. Konish, S. Uechi, H. Tamaki and M. Tako. 2007. Anti-proliferative activity of oversulfated fucoidan from commercially cultured Cladosiphon okamuranus TOKIDA in U937 cells. Int. J. Biol. Macromol., 41, 221-226 https://doi.org/10.1016/j.ijbiomac.2007.02.010
  19. Villarroel, L.H. and A.B. Zanlungo. 1981. Structural studies on the porphyran from Porphyra columbina (Montagne). Carbohydrate Res., 88, 139-145 https://doi.org/10.1016/S0008-6215(00)84608-X
  20. Wang, J., Q. Zhang, Z. Zhang, and Z. Li. 2008. Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica. Int. J. Biol. Macromol., 42, 127-132 https://doi.org/10.1016/j.ijbiomac.2007.10.003
  21. Whistler, R.L. and J.L. Sannella. 1965. Fractional pre- cipitation with ethanol purification of hemicelluloses. In: Methods in carbohydrate chemistry, Vol. 5, R.L. Whistler et al., ed. Academic Press, New Yor
  22. Yaphe, W. and G.P. Arsenault. 1965. Improved resorcinol reagent for the determination of fructose, and of 3,6-anhydrogalactose in polysaccharides. Anal. Bio- chem., 13, 143-148
  23. Yashizawa, Y., A. Ametani, J. Tsunehiro, K. Numura, M. Itoh, F. Fukui and S. Kaminogawa. 1995. Macro- phage stimulation activity of the polysaccharide fraction from a marine algae (Porphyra yezoensis): structure-function relationships and improved solu- bility. Biosci. Biotechnol. Biochem., 59, 1933-1937 https://doi.org/10.1271/bbb.59.1933
  24. Zhang, Q., L. Ning, X. Liu, Zhao, Z.Z. Li and Z. Xu. 2004. The structure of a sulfated galactan from Porphyra haitanensis and its vivo antioxidant activity. Carbohyd. Res., 339, 105-111 https://doi.org/10.1016/j.carres.2003.09.015

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