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Detection of Heterotrophic Dinoflagellate Pfiesteria piscicida (Dinophyceae) in Surface Water Samples Using Real-time PCR

  • Park, Tae-Gyu (Marine Ecology Research Division, National Fisheries Research and Development Institute) ;
  • Kang, Yang-Soon (Marine Ecology Research Division, National Fisheries Research and Development Institute) ;
  • Seo, Mi-Kyung (Marine Ecology Research Division, National Fisheries Research and Development Institute) ;
  • Park, Young-Tae (Marine Ecology Research Division, National Fisheries Research and Development Institute)
  • 발행 : 2008.12.31

초록

Heterotrophic dinoflagellate Pfiesteria piscicida (Dinophyceae) has been claimed to produce potent ichthyotoxins that cause disorientation and eventually death of fish and other marine animals. A real-time PCR probe targeting for SSU rRNA gene was used for detection of P. piscicida in Chinhae Bay, Korea. PCR inhibitors were successfully removed by dilution of template DNA. Positive detections were shown from surface water samples indicating the presence of P. piscicida in Chinhae Bay.

키워드

참고문헌

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