DOI QR코드

DOI QR Code

Effects of Three Dietary Growth Hormones on Growth Performance and Lysozyme Activity in Juvenile Olive Flounder, Paralichthys olivaceus

  • Lee, Seung-Hyung (Department of Aquaculture/Feeds and Foods Nutrition Research Center, Pukyong National University) ;
  • Yoo, Gwang-Yeol (Fishery Research Center) ;
  • Park, Gun-Jun (WooSung CO., LTD) ;
  • Kim, Young-Chul (Department of Aquaculture/Feeds and Foods Nutrition Research Center, Pukyong National University) ;
  • Lee, Jun-Ho (Department of Aquaculture/Feeds and Foods Nutrition Research Center, Pukyong National University) ;
  • Bai, Sung-Chul C. (Department of Aquaculture/Feeds and Foods Nutrition Research Center, Pukyong National University)
  • 발행 : 2008.12.31

초록

In this study, tests were conducted to investigate the effects of three dietary growth hormones, administered in various amounts, on the growth performance and lysozyme activity in juvenile olive flounder, Paralichthys olivaceus. Three dietary growth hormones, recombinant human growth hormone (rHGH), recombinant bovine somatotropin A (rBST A) and recombinant bovine somatotropin B (rBST B) were tested at three different supplemental levels (10, 20 or 40 mg/kg body weight per week) by a $3{\times}3$ factorial design and a complete randomized design in comparison to a control group. Fish were fed one of the ten experimental diets (control, $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{10}$, rBST $A_{20}$, rBST $A_{40}$, rBST $B_{10}$, rBST $B_{20}$ and rBST $B_{40}$) for 6 weeks and afterward were analyzed for growth performance by measuring weight gain (WG), feed efficiency (FE), specific growth rate (SGR) and protein efficiency ratio (PER). Based on the factorial design analysis, fish fed rHGH diets demonstrated significantly higher growth performance than fish fed rBST A or rBST B diets. However there were no significant differences in WG, FE, SGR and PER between fish fed rBST A and rBST B diets. Neither hormone level nor the interaction between the different hormones and their various levels had a significant effect on WG, FE, SGR, PER, lysozyme activity or whole-body proximate composition. A complete randomized design analysis confirmed fish fed $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{10}$, rBST $A_{20}$, rBST $A_{40}$, rBST $B_{20}$ and rBST $B_{40}$ diets for 6 weeks showed higher WG than fish fed the control diet (P<0.05). A higher FE was observed in fish fed $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{20}$ and rBST $A_{40}$ diets in comparison to fish fed the control diet. Fish fed all graded rHGH, rBST A and rBST B supplemented diets showed a higher SGR than fish fed the control diet. Regarding PER, fish fed $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{10}$, rBST $A_{20}$, rBST $A_{40}$ and rBST $B_{20}$ diets were higher than fish fed the control diet. Furthermore, the lysozyme activity of fish fed a diet of $rHGH_{20}$ was significantly higher than that of fish fed any other diet. The results measuring the growth and development of the fish clearly suggest the biopotency of dietary rHGH could be higher than those of both dietary rBST A and rBST B. Further implied is the probability that within the range of 10 to 40 mg/kg BW/week the dietary growth hormones could accelerate growth performance, and that 20 mg rHGH/kg BW/week could possibly enhance lysozyme activity in juvenile olive flounder, Paralichthys olivaceus.

키워드

참고문헌

  1. Adelman, I.R. 1977. Effects of bovine growth hormone on growth of carp (Cyprinus carpio) and the influences of temperature and photoperiod. J. Fish. Res. Board Can., 34, 509-515 https://doi.org/10.1139/f77-082
  2. AOAC (Association of Official Analytical Chemists). 1995. Chap. 4, pages 1-5 in Cunniff P. (ed) Official methods of analysis of the Association of Official Analytical Chemists, 16th edition. Association of Official Analy-tical Chemists, Inc., Arlington, Virginia, USA
  3. Ash, R. 1985. Protein digestion and absorption. Pages 69-93 in Cowey C.B., A.M. Macki and J.G. Bull. (eds) Nutrtition and feeding in Fish. Academic Press, London, UK
  4. Bai, S.C. and D.M. Gatlin III. 1992. Dietary rutin has limited synergistic effects on vitamin C nutrtition of fingerling channel catfish (Ictalurus punctatus). Fish Physiol. Biochem., 10, 183-188 https://doi.org/10.1007/BF00004512
  5. Bai, S.C. and K.W. Kim. 1997. Effects of dietary animal proteins sources on growth and body composition in Korean rockfish, Sebastes schlegeli (written in Korean with English abstract). J. Aquaculture, 10, 77-85
  6. Charmantier, G.M. C-D and D. Aiken. 1989. Accelerating lobster growth with human growth hormone. World Aquaculture, 20, 52-53
  7. Degani, G. and M.L. Gallagher. 1985. Effects of dietary 17$\alpha$-Methyltestosterone and bovine growth hormone on growth and food conversion of slow- and nor-mally-growing American elvers (Anguilla rostrate). Can. J. Fish. Aquat. Sci., 42, 185-189 https://doi.org/10.1139/f85-023
  8. Donaldson, E.M., U.H.M. Fagerlund, D.A. Higgs and J.R. McBride. 1979. Hormonal enhancement of growth in fish. In: W.S. Hoar, D.J. Randall and J.R. Brett (eds), Fish Physiology, Vol. III. Academic Press, New York, NY, 455-597
  9. Foster, A.R., D.F. Houlihan, C. Gray, F. Medale, B. Fauconneau, S.J. Kaushik and P.Y. LeBail. 1991. The effects of bovine GH on protein turnover in rainbow trout. Gen. Comp. Endocrinol., 82, 111-120 https://doi.org/10.1016/0016-6480(91)90302-M
  10. Gill, J.A., J.P. Sumpter, E.M. Donaldson, H.M. Dye, L. Souza, T. Berg, J. Wypych and K. Langley. 1985. Recombinant chicken and bovine growth hormones accelerate growth in aquacultured juvenile Pacific salmon Oncorhynchus kisutch. Biotechnology, 3, 643-646 https://doi.org/10.1038/nbt0785-643
  11. Hertz, Y., A. Tchelet, Z. Madar and A. Gertler. 1991. Absorption of bioactive human growth hormone after oral administration in the common carp (Cyprinus carpio) and its enhancement by deoxycholate. J. Comp. Physiol. B, 161, 159-163
  12. Higgs, D., E.M. Donaldson, H.M. Dye and J.R. McBraide. 1975. A preliminary investigation of the effect of growth hormone on growth and muscle composition of coho salmon (Oncorhynchus kisutch). Gen. Comp. Endocrinol., 27, 240-257 https://doi.org/10.1016/0016-6480(75)90239-7
  13. Higgs, D., E.M. Donaldson, J.R. McBraide and H.M. Dye. 1976. Influence of bovine growth hormone and L-thyroxine on growth, muscle composition and histolo-gical structure of gonads, thyroid, pancreas and pitui-tary of coho salmon (Oncorhynchus kisutch). J. Fish. Res. Board Can., 33, 1585-1603 https://doi.org/10.1139/f76-199
  14. Humbel, R.E. 1984. Insulin-like growth factors, somato-medins, and multiplication stimulating activity: che-mistry. Pages 57-79 in C.H. Li (ed). Hormonal pro-teins and peptides, Vol. 12. Academic Press, New York, USA
  15. Kim, K.W., X.J. Wang and S.C. Bai. 2002. Optimum dietary protein level for maximum growth of juvenile olive flounder, Paralichthys olivaceus (Temminck et Schlegel). Aqua. Res., 33, 673-679 https://doi.org/10.1046/j.1365-2109.2002.00704.x
  16. Kim, K.W., Y.J. Kang, S.M. Choi, X.J. Wang, Y.H. Choi, S.C. Bai and J.Y. Jo. 2005. Optimum dietary protein levels and protein to energy ratios in olive flounder, Paralichthys olivaceus. J. World Aquacult. Soc., 36, 165-178 https://doi.org/10.1111/j.1749-7345.2005.tb00382.x
  17. Leedom, T.A., K. Uchida, T. Yada, N.H. Richman III, J.C. Byatt, R.J. Collier, T. Hirano and E.G. Grau. 2002. Recombinant bovine growth hormone treatment of tilapia: growth response, metabolic clearance, recap-tor binding and immunoglobulin production. Aqua-culture, 207, 359-380 https://doi.org/10.1016/S0044-8486(01)00767-0
  18. Marc, A.M., C. Quentel, A. Severe, P.Y. Le Bail and G. Boeuf. 1995. Changes in some endocrinological and non-specific immunological parameters during sea-water exposure in the brown trout. J. Fish. Biol., 46, 1065-1081 https://doi.org/10.1111/j.1095-8649.1995.tb01410.x
  19. Markert, J.R., D.A. Higgs, H.M. Dye and D.W. Mac-Quarrie. 1977. Influence of bovine growth hormone on growth rate, appetite, and food conversion of yearling coho salmon (Oncorhynchus kisutch) fed two diets of different composition. Can. J. Zool. 55, 74-83 https://doi.org/10.1139/z77-008
  20. Matty, A.J. 1986. Nutrition, hormones and growth. Fish Physiol. Biochem., 2, 141-150 https://doi.org/10.1007/BF02264082
  21. McLean, E., E.M. Donaldson, H.M. Dye and L.M. Souza. 1990. Growth acceleration of coho salmon (On-corhynchus kisutch) following oral administration of recombinant bovine somatotropin. Aquaculture, 91, 197-203 https://doi.org/10.1016/0044-8486(90)90188-S
  22. Nayak, P.K., J. Mishra, K. Kumar and S. Ayyappan. 2003. Effect of human growth hormone (hGH) on growth promotion ion juvenile catfish, Heteropneustes fossilis (Bloch). Indian J. Fish., 50, 167-172
  23. Nissley, S.P. and M.M. Rechler. 1984. Insulin-like growth factors, biosynthesis, receptors and carrier proteins. Pages 128-203 in Li, C.H. (ed). Hormonal proteins and peptides, Vol. 12. Academic Press, New York, USA
  24. Parry, R.M., R.C. Chandau and R.M. Shahani. 1965. A raipd and sensitive assay of muramidase. Proc. Soc. Exp. Biol. Med., 119, 384-386
  25. Peterson, B.C., B.C. Small and B.G. Bosworth. 2004. Effects of bovine growth hormone (Posilac$\circledR$) on growth performance, body composition, and IGFBPs in two stains of channel catfish. Aquaculture, 232, 651-663 https://doi.org/10.1016/S0044-8486(03)00526-X
  26. Peterson, B.C., G.C. Waldbieser and L. Bilodeau. 2005. Effects of recombinant bovine somatotropin on growth and abundance of mRNA for IGF-I and IGF-II in channel catfish (Ictalurus punctatus). J. Anim. Sci., 83, 816-824
  27. Rasmussen, R.S., B. Ronsholdt, T.H. Ostenfeld, E. Mclean and J.C. Byatt. 2001. Growth, feed utilization, carcass composition and sensory characteristics of rainbow trout treated with recombinant bovine placental lacto-gen and growth hormone. Aquaculture, 195, 367-384 https://doi.org/10.1016/S0044-8486(00)00563-9
  28. Statistical Year Book of Food, Agriculture, Forestry and Fisheries. 2008. Statistical Year Book of Food, Agriculture, Forestry and Fisheries. Ministry for Food, Agriculture, Forestry and Fisheries, Korea
  29. Silverstein, J.T., W.R. Wolters, M. Shimizu and W.W. Dickhoff. 2000. Bovine growth hormone treatment of channel catfish: strain and temperature effects on growth, plasma IGF-I levels, feed intake and effi-ciency and body composition. Aquaculture, 190, 77-88 https://doi.org/10.1016/S0044-8486(00)00387-2
  30. Sumpter, J.P. 1992. Control of growth of rainbow trout (Oncorhynchus mykiss). Aquaculture, 100, 229-320
  31. Toullec, J.Y., G. Le Moullac, G. Cuzon and A. Van Wormhoudt. 1991. Immunoreactive human growth hormone like peptides in tropical Penaeids and the effect of dietary hGH on Penaeus vannamei larval development. Aquatic Living Resources, 4, 125-132 https://doi.org/10.1051/alr:1991012
  32. Xu, K., Y. Wei, L. Guo and Z. Zhu. 1991. The effects of growth enhancement of human growth hormone gene transfer and human growth hormone administration on crucian carp (Carassius auratus gibelio, Bloch). Acta Hydrobiologica sinica / Shuisheng Shengwu Xuebao. Wuhan, 15, 103-109
  33. Yada, T., T. Azuma and Y. Takagi. 2001. Stimulation of non-specific immune functions in seawater-acclima-ted rainbow trout, Oncorhynchus mykiss, with re-ference to the role of growth hormone. Comp. Bio-chem. Physiol. B, 129, 695-701 https://doi.org/10.1016/S1096-4959(01)00370-0
  34. Yada, T., K. Muto, T. Azuma and K. Ikuta. 2004. Effects of prolactin and growth hormone on plasma levels of lysozyme and ceruloplasmin in rainbow trout. Comp. Biochem. Physiol. C, 139, 57-63
  35. Yano, T. 1996. The nonspecific immune system: humoral defense. In: Iwama, G., T. Nakanishi (eds), The Fish Immune System: Organism, Pathogen, and Environ-ment. Academic Press, San Diego, 105-107
  36. Yoo, G., S. Lee, G.J. Park, S.M. Choi, O.E. Okorie, Y.C. Kim, K.W. Kim, Y.J. Kang and S.C. Bai. 2007. Effects of dietary recombinant bovine somatotropin levels on growth, plasma recombinant bovine soma-totropin concentrations, and body composition of juvenile Korean rockfish, Sebastes schlegeli. J. World Aquacult. Soc., 38, 200-207 https://doi.org/10.1111/j.1749-7345.2007.00089.x