The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Quantitative Determination of the Marker Constituent of Dioscoreae Rhizoma by the Packaging Methods

포장방법에 따른 산약(山藥) 지표성분의 함량분석

  • Seo, Chang-Seob (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Huang, Dae-Sun (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Lee, Jun-Kyoung (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyoung (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Kim, Ho-Kyoung (Department of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Seo, Young-Bae (Department of Herbology, College of Oriental Medicine) ;
  • Shin, Hyun-Kyoo (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine)
  • 서창섭 (한국한의학연구원 한약제제연구부) ;
  • 황대선 (한국한의학연구원 한약제제연구부) ;
  • 이준경 (한국한의학연구원 한약제제연구부) ;
  • 하혜경 (한국한의학연구원 한약제제연구부) ;
  • 김호경 (한국한의학연구원 한약자원연구부) ;
  • 서영배 (대전대학교 한의과대학 본초학교실) ;
  • 신현규 (한국한의학연구원 한약제제연구부)
  • Published : 2008.12.30
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Abstract

Objectives: To investigate the quantitative determination of marker constituents of Dioscoreae Rhizoma by the packaging methods Methods: HPLC for the determinations of allatoin in the Dioscoreae Rhizoma. the separation method was performed on an Luna $NH_2$ column($250mm{\times}4.6mm,\;5{\mu}m$, Phenomenes) using solvent water-acetonitrile(2:8, v/v%) with photo diode array detector(210nm). The flow rate was 2.0 mL/min. Results: Retention time of allatoin in HPLC chromatogram was about 4.8 min and calibration curve showed good linearity($R^2$=0.9994) at concentrations from 50.0 to 1000.0 ${\mu}g/mL$ of allantoin. Average content of allantoin by packaging methods was $0.16{\sim}0.40%$. In addition. weight loss rate(%) of Dioscoreae Rhizoma according to the packaging methods was $-0.60{\sim}1.80%$. Conclusions: The variation on content of the marker constituent of Dioscoreae Rhizoma by the packaging methods didn't show difference.

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