Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Change of Ratio of Onchungeum Composition Induces Different G1 Arrest Mechanisms in Hep3B Cells

HMC05의 휜쥐를 이용한 단회경구투여 독성시험

  • Shin, Heung-Mook (Department of Physiology, College of Oriental Medicine, Dongguk University)
  • 신흥묵 (동국대학교 한의과대학 생리학교실)
  • Published : 2008.12.25
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Abstract

HCMCO5 is a herbal extract which comprises of eight different herbs. We studied whether this prescription has an acute toxicity in rats. SPF Sprague-Dawley male and female rats were administered orally with HMC05 extract of 2,000 mg/kg for 14days. We examined mortality, clinical signs, body weights and gross findings during the tests. The result showed no dead animals. We also could not find a significant body weight changes during the experimental period. In addition, no differences were found between control and HMC05 treated groups in clinical signs and other findings. These results indicate that HMC05 extract did not show any toxic effects, and oral ADL value was over 2,000 mg/kg in SD rats.

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References

  1. 문국진, 장효일, 김길훤, 신흥묵. HMC05의 혈관이완 활성과 신호전달 작용기전. 동의생리병리학회지 22(2):315-320, 2008.
  2. Ki Mo Kim, Jin Yong Choi, Si-Eun Yoo, Mi Young Park, Bok Soo Lee, Young Hye Ko, Sang Hyun Sung, Heung-Mook Shin, Jeong Euy Park. HMCO5, Herbal Extract, Inhibits NF-${\kappa}B$ Expression in Lipopolysaccharide Treated Macrophages and Reduces Atherosclerotic Lesions in Cholesterol Fed Mice. Journal of Ethnopharmacology. 114: 316-324, 2007. https://doi.org/10.1016/j.jep.2007.08.029
  3. Seung hyun Kim, Eun Jung Choi, Ki young Lee, Sang Hyun Sung, Heung Mook Shin. Simultaneous determination of alkaloids and flavonoids in HMC05 prreparaation by HPLC-DAD. Journal of Liquid Chromatography & Related Technologies, Ethnopharmacology. 31: 2917-2926, 2008.
  4. 식품의약품안전청. 의약품 임상시험 계획 승인 지침. 식품의약품안전청 고시 제2008-32호, 2008.
  5. 식품의약품안전청. 비임상시험관리기준. 식품의약품안전청 고시 제2005-79호, 2005.
  6. 식품의약품안전청. 의약품 등의 독성시험기준. 식품의약품안전청 고시 제2005-60호, 2005.
  7. OECD. OECD Guidelines for the Testing of chemicals. TG 401 & 420, 2002.
  8. Hayes, A.W. Principles and Methods of Toxicology 2nd Edition. New york, Raven Press. p 177, 2001.
  9. Maclean, W., Lyttleton, J. Phlegm Damp. Clinical Handbook of Internal Medicine, 2nd ed. University of Western Sydney Macarthur, Campbeltown, Australia, pp 548-551, 2000.
  10. 이헌제, 성유진, 김상대, 문국진, 김종봉, 김길훤, 신흥묵. 半夏白朮天麻湯의 조성에 따른 혈관이완활성과 기전. 동의생리병리학회지 19(5):1311-1316, 2005.
  11. 장문석, 심경준, 오지훈, 윤지원, 최문정, 김도림, 양웅모, 임형호, 이명종, 박성규. SD계 흰쥐에 대한 마황 추출물의 급성 경구투여 독성 시험 연구. 동의생리병리학회지. 22(4):762- 765, 2008.
  12. 마진열, 황대선, 이남헌, 하혜경, 유영법, 신현규. SD 랫드를 이용한 십전대보탕의 급성 독성 연구. 동의생리병리학회지22(5):1192-1195, 2008.