Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Comparison of Macroscopic Inspection and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) for the Detection of Anisakis simplex complex

고래회충 검출을 위한 육안검사법과 중합효소연쇄반응-제한효소절편길이다형성의 비교

  • Kang, Ju-Hee (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University) ;
  • Lee, Min-Hwa (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University) ;
  • Lee, Kang-Bum (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University) ;
  • Choi, Chang-Sun (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University)
  • 강주희 (중앙대학교 생활과학대학 식품영양학과) ;
  • 이민화 (중앙대학교 생활과학대학 식품영양학과) ;
  • 이강범 (중앙대학교 생활과학대학 식품영양학과) ;
  • 최창순 (중앙대학교 생활과학대학 식품영양학과)
  • Published : 2008.12.31
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Abstract

This research aimed to compare the detection methods of Anisakis simplex in Sea fish by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and macroscopic inspection. We examined 18 Trichiurus lepturus, 11 Scomber japonicus, and 65 Todarodes pacificus collected from the retail markets in the areas of Uljin, Kyuonggi province and Seoul. As the result of examinations, we found that detection rate of Anisakis simplex by macroscopic observation was 89% in Trichiurus lepturus, 90.9% in Scomber japonicus, 32.3% in Todarodes pacificus. The detection rate of Anisakis simplex by PCR-RFLP was 77.7% in Trichiurus lepturus, 81.8% in Scomber japonicus, 26.1% in Todarodes pacificus. We could conclude that PCR-RFLP method of Anisakis simplex was more specific rather than macroscopic observation.

Keywords

References

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