Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effects of enamel matrix derivatives on biologic activities of human periodontal fibloblasts to demineralized root surface

법랑기질유도체가 탈회 치근표면에서 치주인대섬유아세포의 생물학적 성상에 미치는 영향

  • Lee, Kang-Woon (Department of Periodontology, School of dentistry, Seoul National University) ;
  • Kim, Tae-Il (Department of Periodontology, School of dentistry, Seoul National University) ;
  • Seol, Yang-Jo (Department of Periodontology, School of dentistry, Seoul National University) ;
  • Lee, Yong-Moo (Department of Periodontology, School of dentistry, Seoul National University) ;
  • Ku, Young (Department of Periodontology, School of dentistry, Seoul National University) ;
  • Rhyu, In-Chul (Department of Periodontology, School of dentistry, Seoul National University) ;
  • Chung, Chong-Pyung (Department of Periodontology, School of dentistry, Seoul National University) ;
  • Han, Soo-Boo (Department of Periodontology, School of dentistry, Seoul National University)
  • 이강운 (서울대학교 치의학대학원 치주과학교실) ;
  • 김태일 (서울대학교 치의학대학원 치주과학교실) ;
  • 설양조 (서울대학교 치의학대학원 치주과학교실) ;
  • 이용무 (서울대학교 치의학대학원 치주과학교실) ;
  • 구영 (서울대학교 치의학대학원 치주과학교실) ;
  • 류인철 (서울대학교 치의학대학원 치주과학교실) ;
  • 정종평 (서울대학교 치의학대학원 치주과학교실) ;
  • 한수부 (서울대학교 치의학대학원 치주과학교실)
  • Published : 2008.12.31
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Abstract

Purpose: The aim of this study was to investigate the effects of EMD on demineralized root surface using human periodontal ligament cells and compare the effects of root conditioning materials(tetracycline(TCN), EDTA). Material and Methods: Dentin slices were prepared from the extracted teeth and demineralized with TCN and EDTA. Demineralized dentin slices were incubated at culture plate with 25, 50 and $100{\mu}g/ml$ concentration of EMD. Cell attachment, alkaline phosphatase activity test, protein synthesis assay and scanning electronic microscopic examination were done. Results: Cells were attached significantly higher in EMD treated group at 7 and 14 days. Cell numbers were significantly higher in EMD treated group. Alkaline phosphatase activity was significantly higher in EMD treated group at 7 and 14 days. Protein synthesis was significantly higher in EMD treated group at 7 and 14 days. Conclusion: Enamel matrix derivatives enhance the biologic activities of human periodontal ligament cells on demineralized root surface and its effects are dependent on the concentration of EMD.

Keywords

References

  1. Peter Eickholz. Long term result of guided tissue regeneration therapy with nonresorbable and bioabsorbable barrier. J Periodontol 2001;72:35-42 https://doi.org/10.1902/jop.2001.72.1.35
  2. Hoang AM, Klebe RJ, Steffensen B, et al. Amelogenin is a cell adhesion protein. J Dent Res 2002;81:497-500 https://doi.org/10.1177/154405910208100713
  3. Van der Pauw MT, Van den Bos T, Everts V, Beertsen W. Enamel matrix derived protein stimulates attachment of periodontal ligament fibroblasts and enhances alkaline phosphatase activity and transforming growth factor ${\beta}_1$release of periodontal ligamental and gingival fibroblasts. J Periodontol 2000;71:31-43 https://doi.org/10.1902/jop.2000.71.1.31
  4. Stuart J. Froum. A comparative study utilizing open flap debridement with and without enamel matrix derivative in the treatment of periodontal intrabony defects. J Periodontol 2001;72:25-34 https://doi.org/10.1902/jop.2001.72.1.25
  5. Hakki SS, Berry JE, Somerman MJ. The effect of enamel matrix protein derivative on follicle cells in vitro. J Periodontol 2001;72:679-687 https://doi.org/10.1902/jop.2001.72.5.679
  6. Hoang AM, Oates TW, Cochran DL. In vitro wound healing responses to enamel matrix derivative. J Periodontol 2000;71:1270-1277 https://doi.org/10.1902/jop.2000.71.8.1270
  7. Kawase T, Okuda K, Yoshie H, Burns DM. Cytostatic action of enamel matrix derivative (EMDOGAIN) on human oral squamous cell carcinoma-derived SCC25 epithelial cells. J Periodontal Res 2000;35:291-300 https://doi.org/10.1034/j.1600-0765.2000.035005291.x
  8. Pitaru S, Melcher AH. Organization of an oriented fiber system in vitro by human gingival fibroblast attached to dental tissue: relationship between cells and mineralized and demineralized tissue. J Periodontal Res 1987;22:6-13 https://doi.org/10.1111/j.1600-0765.1987.tb01533.x
  9. Madison JG 3rd, Hokett SD. The effects of different tetracyclines on the dentin root surface of instrumented, periodontally involved human teeth: a comparative scanning electron microscopy study. J Periodontol 1997;68:739-745 https://doi.org/10.1902/jop.1997.68.8.739
  10. Behavior of human periodontal ligament cells on CO2 laser irradiated dentinal root surfaces: an in vitro study. J Periodon Res 2004;39:373-379 https://doi.org/10.1111/j.1600-0765.2004.00751.x
  11. Hammarstrom L, Gestrelius S. Periodontal regeneration in a buccal dehiscence model in monkeys after application of enamel matrix proteins. J Clin Periodontol 1997;24: 669-677 https://doi.org/10.1111/j.1600-051X.1997.tb00248.x
  12. Gestrelius S, Andersson C, Lidstrom D, Hammarstom L, Somerman M. In vitro studies on periodontal ligament cells and enamel matrix derivative. J Clin Periodontol 1997;24:685-692 https://doi.org/10.1111/j.1600-051X.1997.tb00250.x
  13. Lyngstadaas SP, Lunderberg E, Ekdahl H, Andersson C, Gestrelius S. Autocrine growth factors in human periodontal ligamental cells cultured on enamel matrix derivative. J Clin Periodontol 2001;28:181-188 https://doi.org/10.1034/j.1600-051x.2001.028002181.x
  14. Haase HR, Bartold PM. Enamel matrix induces matrix synthesis by cultured human periodontal fibroblast cells. J Periodontol 2001;72:341-348 https://doi.org/10.1902/jop.2001.72.3.341
  15. Cattaneo V, Rota C, Silverstri M, et al. Effect of enamel matrix derivative on human periodontal fibroblast: proliferation, morphology and root surface colonization. An in vitro study. J Periodont Res 2003;38:568-574 https://doi.org/10.1034/j.1600-0765.2003.00690.x
  16. Chano L, Tenenbaum HC, Lekic PC, Sodek J, McCulloch CA. Emdogain regulation of cellular differentiation in wounded rat periodontium. J Periodontal Res 2003;38: 164-174 https://doi.org/10.1034/j.1600-0765.2003.00003.x
  17. Ohyama M, Suzuki N, Yamaguchi Y, et al. Effect of enamel matrix derivative on the differentiation of C2C12 cells. J Periodontol 2002;73:543-550 https://doi.org/10.1902/jop.2002.73.5.543
  18. You-Seok Kim, Hyun-Seon Jang, Ju-chol Park, et al. The effect of enamel matrix derivative(EMD) in combination with deproteinized bovine bone material(DBBM) on the early wound healing of rabbit calvarial defects. J Korean Acad Periodontol 2005;35:199-216 https://doi.org/10.5051/jkape.2005.35.1.199
  19. Chong CH, Carnes DL, Moritz AJ, et al. Human periodontal fibroblast response to enamel matrix derivative, amelogenin, and platelet-derived growth factor-BB. J Periodontol 2006;77:1242-1252 https://doi.org/10.1902/jop.2006.050147
  20. Pischon N, Zimmermann B, Bernimoulin JP, Hagewald S. Effects of enamel matrix derivative on human osteoblasts and PDL cells grown in organoid cultures. Oral Surg Oral Med Oral Pathol Oral Radiol Endodod 2006;102:551-557 https://doi.org/10.1016/j.tripleo.2005.08.040
  21. Davenport DR, Mailhot JM, Wataha JC, et al. Effects of enamel matrix protein application on the viability, proliferation, and attachment of human periodontal ligament fibroblasts to diseased root surfaces in vitro. J clin Periodontol 2003;30:125-131 https://doi.org/10.1034/j.1600-051X.2003.00150.x
  22. Jeong YJ, Kim KW, Kim TI, et al. The Effects of enamel matrix derivatives on the proliferation and gene expression of PDL fibroblast, $SaOs_2$ cells and Cementum derived cells. J Korean Acad Periodontol 2005;35:321-333 https://doi.org/10.5051/jkape.2005.35.2.321
  23. Kim DW, Chung CH, Lim SB, Ko SY. Effects of enamel matrix protein derivatives on the periodontal ligament like fibroblast and osteoblast like cells. J Korean Acad Periodontol 2003;33:225-246 https://doi.org/10.5051/jkape.2003.33.2.225
  24. Rincon JC, Haase HR, Bartold PM. Effect of Emdogain on human periodontal fibroblasts in an in vitro wound-healing model. J Periodontal Res 2003;38:290-295 https://doi.org/10.1034/j.1600-0765.2003.00610.x
  25. Schlueter SR, Carnes DL, Cochran DL. In vitro effects of enamel matrix derivative on microvascular cells. J Periodontol 2007;78:141-151 https://doi.org/10.1902/jop.2007.060111
  26. Yuan K, Hsu CW, Tsai WH. The induction and possible subsequent effect of human antibodies against porcine enamel matrix derivative. J Periodontol 2006;77:1355-1361 https://doi.org/10.1902/jop.2006.050321
  27. St George G, Darbar U, Thomas G. Inflammatory external root resorption following surgical treatment for intrabony defects: a report of two cases involving Emdogain and a review of literature. J Clin Periodontol 2006;33:449-454 https://doi.org/10.1111/j.1600-051X.2006.00926.x
  28. Rodrigues TL, Marchesan JT, Coletta RD, et al. Effects of enamel matrix derivative and transforming growth factor-beta 1 on human periodontal ligament fibroblasts. J Clin Periodontol 2007;34:514-522. https://doi.org/10.1111/j.1600-051X.2007.01090.x
  29. Gao J, Symons AL, Haase H, Bartold PM. Should cementoblasts express alkaline phosphatase activity? Preliminary study of rat cementoblasts in vitro. J Periodontol 1999;70:951-959 https://doi.org/10.1902/jop.1999.70.9.951
  30. Tenorio D, Cruchley A, Hughes FJ. Immunocytochemical investigation of the rat cementoblast phenotype. J Periodon Res 1993;28:411-419