Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effects of $H_2O_2$ and chlorhexidine on MMP-1, TIMP-1,2, Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts

사람 치주인대섬유모세포에서 MMP-1, TIMP-1,2, Type 1 collagen, fibronectin 및 UNCL 발현에 미치는 $H_2O_2$와 chlorhexidine의 효과

  • Choi, Seong-Mi (Department of Periodontology, School of Dentistry, Cho Sun University) ;
  • Jang, Hyun-Seon (Department of Periodontology, School of Dentistry, Cho Sun University) ;
  • Kim, Byung-Ock (Department of Periodontology, School of Dentistry, Cho Sun University)
  • 최성미 (조선대학교 치의학전문대학원 치주과학교실) ;
  • 장현선 (조선대학교 치의학전문대학원 치주과학교실) ;
  • 김병옥 (조선대학교 치의학전문대학원 치주과학교실)
  • Published : 2008.12.31
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Abstract

Purpose: To evaulate the effects of chlorhexidine and $H_2O_2$ on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase(TIMP-1, TIMP-2), Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts (hPDLF). Materials and Methods: $1.2{\times}10^{-1}%$, $1.2{\times}10^{-2}%$ and $1.2{\times}10^{-3}%$ CHX and $3{\times}10^{-3}%$, $3{\times}10^{-4}%$ and $3{\times}10^{-5}%$ $H_2O_2$ and mixture of CHX and $H_2O_2$ were applied to hPDLF for 1 min and 30 min. The mRNA expressions of MMP-1, TIMP-1 and 2, Type 1 collagen, fibronectin and UNCL in hPDLF were analysed by RT-PCR. Results: The result were as follows: 1. The expression of UNCL mRNA was higher than that of other mRNAs. 2. $1.2{\times}10^{-3}%$ CHX increased mRNA expressions of hPDLF as application time increased. 3. $H_2O_2$ lower than $3{\times}10^{-3}%$ increased expression of UNCL mRNA, and did not decrease mRNA expression of hPDLF. 4. hPDLF treatment with $1.2{\times}10^{-1}%$ CHX (with or without $H_2O_2$) resulted in no gene expression. 5. hPDLF treatment with $1.2{\times}10^{-2}%$ CHX (with or without $H_2O_2$) for 30 minutes resulted in no gene expression. Conclusion: Because low concentration of CHX and $H_2O_2$ increased UNCL mRNA expression of hPDLF, low concentraction of CHX and $H_2O_2$ may have an antioxidative effect.

Keywords

References

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